Cigarette Smoke-Induced Oxidative Stress and Autophagy in Human Alveolar Epithelial Cell Line (A549 Cells)

Szoka, Piotr; Lachowicz, Jean E.; Ćwiklińska, Magdalena; Łukaszewicz, Aleksander; Rybak, Agnieszka; Baranowska, Urszula; Hołownia, Adam

doi:10.1007/5584_2019_373

“…Under physiological conditions, the enzymes and nonenzymatic substances in these metabolisms maintain a dynamic equilibrium state of oxidation-antioxidation and commonly participate in a variety of normal physiological functions [8,9]. However, due to the imbalance between prooxidant and antioxidant components, in many processes, such as inflammation and carcinogenesis, oxidative stress (OxS) is accompanied by an increase in ROS production and/or a decrease in antioxidant levels in target cells and tissues [9][10][11]. The intracellular oxidative damage is mainly caused by FR and ROS [12][13][14], which leads to the oxidative damage of protein and DNA [14,15].…”

Section: Introductionmentioning

confidence: 99%

The Relationship between Serum Trace Elements and Oxidative Stress of Patients with Different Types of Cancer

Yang

¹

,

Dai

²

,

Chen

³

et al. 2021

Oxidative Medicine and Cellular Longevity

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Objective. Many studies have identified causal and promotive roles of oxidative stress (OxS) and oxidative damage caused by OxS in the occurrence and progression of cancer. Many biomarkers in the blood circulation of patients may change correspondingly with the development of tumors. This study is aimed at investigating the correlation between OxS and serum trace element (TE) levels of patients with different types of cancer. Methods. 1143 different types of cancer patients and 178 healthy controls from Mar. 2018 to Aug. 2020 in Mianyang Central Hospital were involved in this study. Their levels of OxS parameters (including total oxidant status (TOS), total antioxidant status (TAS), and oxidant stress index (OSI)) and the concentrations of serum TEs (including Cu, Zn, Fe, and Se) were determined. Results. Compared with healthy controls, all types of cancer patients had higher TOS level (all P adj < 0.001 ) and OSI level ( z = 6.228 ~ 9.909 , all P adj < 0.001 ) and lower TAS level (all P adj < 0.001 ). Compared with healthy controls, the changes of four TE levels in serum were different in different types of cancer patients, among which Cu increased in all groups, but there was no statistical difference in gastric and brain cancer; Se decreased in all groups, but there was no statistical difference in gastric, colorectal, esophageal, and other cancer; Zn was significantly decreased in breast cancer patients ( P adj < 0.001 ); there was no statistical difference in the change of Fe in liver, kidney, and other cancer. Spearman correlation showed that the change of Cu concentration was most closely related to the three OxS parameters and was strongly correlated in the observed several types of tumors ( r s > 0.6 ). Multinomial logistic regression showed that the risks of different tumors are related to the level change of multiple TEs and OxS parameters ( OR TOS = 1.19 ~ 2.82 , OR OSI = 2.56 ~ 4.70 , OR TAS = 0.20 ~ 0.46 , OR Cu = 0.73 ~ 1.44 , OR Zn = 0.81 ~ 0.91 , OR Fe = 0.68 ~ 1.18 , and OR Se = 0.22 ~ 0.45 , all P < 0.006 ). Conclusions. The OxS exists in the occurrence and development of cancer, which may be related to the changes of certain trace elements. In order to evaluate OxS correctly, it is necessary to detect TAS and TOS and at the same time, their ratio OSI should be detected. Assessment of markers representing the overall level of OxS and TEs may guarantee improved the monitoring of disease occurrence and development risk in cancer patients.

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“…Tobacco smoke exposure can cause cytotoxicity [17,18], inflammation [19,20], oxidative stress [21] and apoptosis [22]. In this study, CC and HTP smoke traps were applied to human bronchial epithelial cells, that the cell survival rate gradually decreased and even cell death occurred with the increase of dose.…”

Section: Discussionmentioning

confidence: 95%

The Biological Effects of Toxic Dose Combustion Cigarettes and Heated Tobacco Products in BEAS-2B and A549 Cells

Liu,

Yan,

Li

et al. 2024

life

0

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The use of novel tobacco products that do not burn tobacco leaves is becoming increasingly popular worldwide. In terms of respiratory system, smoking damage to the epithelial cells of lung is in urgent need of attention and research. In this study, we produced the combustion cigarettes (CC) condensate from conventional cigarettes and the heated tobacco products (HTP) condensate from heat-not-burn (HNB) tobacco, and determine the nicotine and other ingredients content respectively to assessment the CC and HTP cytotoxicity after acute exposure to cigarette smoke. We further select a toxicological dose for comparison of the difference between the CC and HTP, which the impacts on BEAS-2B and A549 cells at a 70% survival rate was evaluated. Then, biological effects of CC and HTP on BEAS-2B and A549 cells, which including cell viability, apoptosis and cell cycle were investigated. Results showed that the BEAS-2B and A549 cells survival rate was decreased with increasing dose of CC and HTP smoke trap for 24 h. Both CC and HTP could suppress the proliferation of BEAS-2B cells, though the degree of inhibition of cell proliferation in CC group was greater than in HTP group. The CC and HTP traps could promote apoptosis in BEAS-2B and A549 cells. The apoptosis rate of HTP group was less than that of CC group. In addition, CC and HTP traps could partly restrain cell cycle progression in G0/G1 phase. These results demonstrate that CC and HTP can cause cytotoxicity, apoptosis and affect cell cycle progression, which thus may be useful to elucidate the underlying biological effects of novel tobacco products and provide new ideas for the preventing and treating diseases.

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“…[16][17][18][19] Studies have shown that these effects may be due to the release of reactive oxygen species that cause damage at the cellular and tissue levels. Smoking also affects normal tissue perfusion, [20,21] impairs microcirculation, [22][23][24] increases oxidative stress, induces vascular injury, [25] reduces red blood cell velocity in the mesenteric vasculature and enhances venule pressure. [26] The decreased microcirculation and insufficient perfusion as well as vascular injury in patients with a history of smoking may contribute to POI after radical resection of rectal cancer; however, this hypothesis needs to be investigated in further studies.…”

Section: Discussionmentioning

confidence: 99%

Smoking is a risk factor for postoperative ileus after radical resection in male patients

Wang

¹

,

Guo

²

,

Cui

³

et al. 2021

Medicine

2

0

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Most smokers are males, and smoking has been indicated as a risk factor for many cancers as well as postoperative complications after cancer surgery. However, little is known about whether smoking is a risk factor for postoperative ileus (POI) after radical rectal cancer resection in males. The aim of this study was to assess whether smoking is a risk factor for POI after radical resection in male rectal cancer patients. Data of 1486 patients who underwent radical resection for rectal cancer were extracted from the clinical medical system in our hospital and were statistically analyzed. POI was defined as nausea, vomiting or pain, failure to have bowel function for more than 4 days postoperatively, and absence of a mechanical bowel obstruction. The rate of POI was 12.79%. Univariate analysis showed that patients in the POI group were more likely to have a history of smoking and drinking and receive intraperitoneal chemotherapy and had a larger intraperitoneal chemotherapy dosage. In the multivariable analysis, smoking remained significantly associated with a higher incidence of POI (OR 2.238, 95% CI [1.545–3.240], P = .000). The results also showed that patients who received postoperative patient-controlled intravenous analgesia had a lower incidence of POI. Male patients with a history of smoking who undergo elective radical resection for rectal cancer have an increased risk for POI complications.

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Cigarette Smoke-Induced Oxidative Stress and Autophagy in Human Alveolar Epithelial Cell Line (A549 Cells)

Cited by 10 publications

References 27 publications

The Relationship between Serum Trace Elements and Oxidative Stress of Patients with Different Types of Cancer

The Relationship between Serum Trace Elements and Oxidative Stress of Patients with Different Types of Cancer

The Biological Effects of Toxic Dose Combustion Cigarettes and Heated Tobacco Products in BEAS-2B and A549 Cells

Smoking is a risk factor for postoperative ileus after radical resection in male patients

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