Cigarette Smoke-Induced Placental Adrenomedullin Expression and Trophoblast Cell Invasion

Kraus, Daniel; Feng, Liping; Heine, R. Phillips; Brown, Haywood L.; Caron, Kathleen M.; Murtha, Amy P.; Grotegut, Chad A.

doi:10.1177/1933719113488456

Cited by 14 publications

(10 citation statements)

References 38 publications

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“…Due to this property, ADM has been intensively studied in pregnancy and preeclampsia as a potential pathogenic factor in this disease. [103][104][105][106][107][108][109][110][111][112] It is speculated to play a role in trophoblast implantation and angiogenesis. 113 Indeed, we and others have shown that ADM mediates EVT growth, migration, invasion, and STB function.…”

Section: Gene Expression Profiles In Pfbstreated Cellsmentioning

confidence: 99%

Perfluorobutane sulfonate exposure disrupted human placental cytotrophoblast cell proliferation and invasion involving in dysregulating preeclampsia related genes

et al. 2020

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We reported that maternal PFBS, an emerging pollutant, exposure is positively associated with preeclampsia which can result from aberrant trophoblasts invasion and subsequent placental ischemia. In this study, we investigated the effects of PFBS on trophoblasts proliferation/invasion and signaling pathways. We exposed a human trophoblast line, HTR8/SVneo, to PFBS. Cell viability, proliferation, and cell cycle were evaluated by the MTS assay, Ki-67 staining, and flow cytometry, respectively. We assessed cell migration and invasion with live-cell imaging-based migration assay and matrigel invasion assay, respectively. Signaling pathways were examined by Western blot, RNA-seq, and qPCR. PFBS exposure interrupted cell proliferation and invasion in a dose-dependent manner. PFBS (100 μM) did not cause cell death but instead significant cell proliferation without cell cycle disruption. PFBS (10 and 100 μM) decreased cell migration and invasion, while PFBS (0.1 μM) significantly increased cell invasion but not migration. Further, RNA-seq analysis identified dysregulated HIF-1α target genes that are relevant to cell proliferation/invasion and preeclampsia, while Western Blot data showed the activation of HIF-1α, but not Notch, ERK1/2, (PI3K)AKT, and P38 pathways. PBFS exposure altered trophoblast cell proliferation/invasion which might be mediated by preeclampsia-related genes, suggesting a possible association between prenatal PFBS exposure and adverse placentation. K E Y W O R D S invasion, PFBS, placenta cytotrophoblast, preeclampsia, proliferation | 14183 MARINELLO Et AL. 1 | INTRODUCTION Poly-and per-fluoroalkyl substances (PFAS) have attracted widespread attention in recent years due to their bioaccumulation, toxicity, and ubiquitous nature. 1 PFAS are a group of compounds characterized by a hydrophobic poly-fluorinated alkyl chain and a polar hydrophilic terminal functional group. PFAS are used in a variety of industrial and consumer products such as surfactants for soil/stain resistance, textiles, paper and metals, firefighting foam, and pesticides. 2,3 Humans are exposed to PFAS through contaminated drinking water, food, outdoor air, indoor dust, and soil. 4 One of the most widely known PFAS is perfluorooctane sulfonic acid (PFOS), which has an eight-carbon backbone with a sulfonate. Due to strong carbon-fluorine bonds (C8), PFOS is extremely stable and persistent in the environment (USEPA, Document# 822R14002) and is not readily eliminated from humans due to its half-life of 5.4 years. 5-16 Data from human and animal studies demonstrate numerous health and ecological risks resulting from PFOS exposure including increased risk of thyroid disease, blood cholesterol levels, and preeclampsia and decreased body's response to vaccine, fertility in women, and birth weight, liver, and immune system damage. 17-29 Thus, beginning in 2002, most manufacturing of PFOS in the United States was discontinued voluntarily by 3M and DuPont in favor of shorter chain PFAS (C4 or C6, Toni Krasnic, the U. S. Environmental Protection A...

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Section: Gene Expression Profiles In Pfbstreated Cellsmentioning

confidence: 99%

Perfluorobutane sulfonate exposure disrupted human placental cytotrophoblast cell proliferation and invasion involving in dysregulating preeclampsia related genes

et al. 2020

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“…Cigarette smoke extract increases the invasiveness of trophoblast cells in vitro and in vivo by enhancing adrenomedullin expression. Co-treating the cultured cells with adrenomedullin inhibitor significantly attenuates this effect [ 73 , 74 ].…”

Section: Studies Concerning Association Between Lcpufa and Preeclamentioning

confidence: 99%

Omega-3 Long-Chain Polyunsaturated Fatty Acids and Preeclampsia: Trials Say “No,” but Is It the Final Word?

2017

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Preeclampsia is a dangerous disorder of pregnancy, defined as hypertension with proteinuria. Its nature remains elusive, and measures of prevention and treatment are limited. Observational studies have suggested that preeclampsia is associated with low intake of omega-3 long-chain polyunsaturated fatty acids (LCPUFA). In recent decades, researchers studied LCPUFA supplementation as a measure to prevent preeclampsia. Most of these trials and later systematic reviews yielded negative results. However, these trials had several important limitations associated with heterogeneity and other issues. Recent research suggests that preeclampsia trials should take into consideration the gender of the fetus (and thus sexual dimorphism of placenta), the positive effect of smoking on preeclampsia prevalence, and the possibility that high doses of LCPUFA mid-term or later may promote the disorder instead of keeping it at bay. In this review, we discuss these issues and future prospects for LCPUFA in preeclampsia research.

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“…Thus there is currently major interest and effort in the field, to expand our understanding of the factors that Here, we have emphasized that both maternal and fetal-derived ADM is important for establishing and maintaining a successful pregnancy. [15] …”

Section: Adm In Uteroimplantation Growthmentioning

confidence: 99%

Adrenomedullin Antagonist Infusion Causes Uteroimplantation Growth Restriction

Josthna¹

2017

WJPPS

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The Adrenomedullin peptide hormone has a potent vasorelaxant activity. This novel peptide has shown to be almost a ubiquitous peptide, with the number of tissues and cell types synthesizing adrenomedullin. The pattern of ADM level and gene expression of ADM, its receptor components from early pregnancy has been studied in our present work. In our previous papers we have studied whether antagonism of endogenous adrenomedullin in rats during early gestation period emanated in diminished uterus and implantation growth and found whether this causes through induction of apoptosis.Rats on gestational day 2 were implanted subcutaneously with osmotic (Alzet) minipumps delivering 125 and 250µg rat/ day/ of AM 22-52 and were killed on gestational day 9. This review summarizes a clear cut view of Adrenomedullin receptor and significance of ADM in circulation during gestation period.

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Cigarette Smoke-Induced Placental Adrenomedullin Expression and Trophoblast Cell Invasion

Cited by 14 publications

References 38 publications

Perfluorobutane sulfonate exposure disrupted human placental cytotrophoblast cell proliferation and invasion involving in dysregulating preeclampsia related genes

Perfluorobutane sulfonate exposure disrupted human placental cytotrophoblast cell proliferation and invasion involving in dysregulating preeclampsia related genes

Omega-3 Long-Chain Polyunsaturated Fatty Acids and Preeclampsia: Trials Say “No,” but Is It the Final Word?

Adrenomedullin Antagonist Infusion Causes Uteroimplantation Growth Restriction

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