Cigarette smoke induces anaphase bridges and genomic imbalances in normal cells

Luo, Li; Werner, Kristen M.; Gollin, Susanne M.; Saunders, William S.

doi:10.1016/j.mrfmmm.2004.06.031

Cited by 75 publications

(47 citation statements)

References 51 publications

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“…Micronucleus formation was determined by the acentric fragments or whole chromosomes that were not incorporated to the main nucleus during cell division cycle [24]. Chromosome bridges is either due to chromosome stickiness producing abnormal anaphase separation or may be attributed to unequal translocation or inversion of chromosome segments and also due to breakage and fusion of chromosome or chromatids [25]. The chromosomal fragments are more common in metaphase and anaphase.…”

Section: Resultsmentioning

confidence: 99%

Genotoxic effect of distillery effluent on root tip cells of Allium sativum L.

2015

J App Biol Biotech

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In the current study an attempt has been made to evaluate the cytotoxic efficacy of distillery effluent on somatic cells of Allium sativum. The garlic bulbs were treated with different concentrations of effluent viz. 2.5, 5, 7.5 and 10% at room temperature. The percentage mitotic index was found to be decreased significantly as the concentration of the effluent increased, except in 2.5% concentration, where the mitotic index is higher than control. The chromosomal abnormalities were found to be increased as the effluent concentration increased when compared to control. The observed chromosomal aberrations were laggards, fragmented chromosome, fragmented anaphase and metaphase, chromosomal bridges, micronuclei, lobed nucleus, scattered chromosome, disoriented anaphase, Polyploidy cells. According to present findings 2.5% concentration of the effluent was found to enhance the rate of cell division when compare to control on the contrary higher of the effluent showed negative effects on mitotic division in somatic cells of Garlic.

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Section: Resultsmentioning

confidence: 99%

Genotoxic effect of distillery effluent on root tip cells of Allium sativum L.

2015

J App Biol Biotech

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“…Anaphase bridges could happen during the translocation of the unequal chromatid exchange, due to dicentric chromosome presence, or due to the breakage and fusion of chromosomes and chromatids. (El-Ghamery et al 2000;Luo et al 2004). In addition, bridges may arise due to chromosome breaks, stickiness and breakage and reunion of the broken ends (Badr et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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“…It has also been suggested that the carcinogenic potential of HPV increases with viral integration with host DNA (Spence et al, 2005). The process of integration occurs at fragile sites or hot spots of DNA breakage, and there is evidence that tobacco smoking induces DNA breaks in human cells (Luo et al, 2004). Hence, an increased frequency of HPV integration in smokers may increase the risk of carcinogenesis in the presence of HPV infection.…”

Section: Discussionmentioning

confidence: 99%