Cigarette Smoking and Alcohol Ingestion as Risk Factors for Laryngeal Squamous Cell Carcinoma at Kenyatta National Hospital, Kenya

Menach, Pyeko; Oburra, Herbert; Patel, Asmeeta

doi:10.4137/cment.s8610

Cited by 22 publications

(22 citation statements)

References 29 publications

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“…Laryngeal cancer is the highest incidence of head and neck cancer and probably accounts for 1%～5% cancer incidence worldwide . Smoking and alcohol are considered as common major risk factors for laryngeal cancer . Moreover, some researchers have found that people with a family history of cancer are also susceptible to laryngeal cancer, which implicates that genetic factors serve an important role in the occurrence and progression of laryngeal cancer .…”

Section: Introductionmentioning

confidence: 99%

LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis

Zhang

Wang

et al. 2019

J Cellular Molecular Medi

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Long non‐coding RNA (LncRNA) MEG3 serves a regulatory role in the progression of several types of cancer, but the role of MEG3 in laryngeal cancer is still unknown. The aim of this study was to explore the regulatory role and mechanism of MEG3 in laryngeal cancer. MEG3 expression in 50 laryngeal cancer tissue samples was detected by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). The effects of MEG3 overexpression on laryngeal cancer cells were investigated in vitro and in vivo. The mechanism of competitive endogenous RNA (ceRNA) was validated through luciferase reporter assay, RT‐qPCR and Western blotting. MEG3 was down‐regulated in laryngeal cancer tissues, and the low MEG3 expression was associated with advanced clinical stage. Additionally, MEG3 overexpression inhibited the proliferation and induced the apoptosis of laryngeal cancer cells in vitro and in vivo. Particularly, MEG3 bound to miR‐23a specifically and a reciprocal negative regulation existed between miR‐23a and MEG3. Moreover, MEG3 up‐regulated apoptotic protease activating factor‐1 (APAF‐1), a known miR‐23a's target, thereby leading to the activation of caspase‐9 and caspase‐3. Meanwhile, these activated effects were rescued by miR‐23a overexpression. In conclusion, the present study demonstrated that MEG3 functions as a novel tumour suppressive LncRNA in laryngeal cancer for the first time. Furthermore, MEG3 may act as a ceRNA to regulate APAF‐1 expression by competitive binding to miR‐23a, thereby regulating the progression of laryngeal cancer.

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Section: Introductionmentioning

confidence: 99%

LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis

Zhang

Wang

et al. 2019

J Cellular Molecular Medi

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“…Cancer of the larynx accounts for ~1.2% of new malignancies worldwide every year, with an incidence of ~151,000 cases per year, ~95% of these tumors have squamous cell carcinoma as the primary histological type (1,2). It has been reported that this neoplasia is strongly linked to cigarette smoking, alcohol ingestion and human papillomavirus infection (3)(4)(5). Treatment options in laryngeal carcinoma comprise surgery, radiotherapy, chemotherapy or a combination therapy.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-93 regulates cyclin G2 expression and plays an oncogenic role in laryngeal squamous cell carcinoma

Xiao

Zhou

Cao

et al. 2014

International Journal of Oncology

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microRNA93 (miR-93) is expressed in the miR‑106b-25 cluster, located in intron 13 of the MCM7 gene. Our previous study found that miR-93 was significantly upregulated in laryngeal squamous cell carcinoma (LSCC), and cyclin G2 (CCNG2) was a potential target of miR-93 in LSCC. However, the possible functions and molecular mechanisms of miR-93 in LSCC remain unknown. In the present study, we show that the level of CCNG2 protein expression was significantly lower in LSCC cancer tissue than normal tissues. The level of CCNG2 was correlated with clinical stages, lymph node metastasis and histological grade. We further show that the expression level of miR-93 was inversely correlated with CCNG2 expression in clinical specimens. Furthermore, gain-of-function assays revealed that miR-93 promoted cell proliferation, decreased apoptosis rates, induced cell cycle arrest and promoted cell migration and invasion, whereas silencing of miR-93 attenuated these carcinogenic processes. In addition, overexpression of miR-93 in Hep-2 cells could reduce the mRNA and protein levels of CCNG2, whereas silencing of miR-93 in Hep-2 cells significantly increased CCNG2 expression. A luciferase assay verified that miR-93 could bind to the 3' untranslated region of CCNG2. Importantly, ectopic expression of CCNG2 in miR-93 cells rescued the effect of miR-93 on LSCC proliferation. Knockdown of CCNG2 promoted cell proliferation resembling that of miR-93 overexpression. These findings demonstrated that miR-93 promotes tumor growth by directly suppressing CCNG2. Taken together, these results suggested that this newly identified miR-93-CCNG2 axis may be involved in LSCC proliferation and progression. Our findings provide novel potential targets for LSCC therapy and prognosis.

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“…The risk imparted by both factors has been shown to be synergistic thus increasing the risk of squamous cell carcinoma by many fold [4, 9, 10]. However, alcohol as an independent risk factor for laryngeal cancer remains controversial [10].…”

Section: Introductionmentioning

confidence: 99%

Demography and Histologic Pattern of Laryngeal Squamous Cell Carcinoma in Kenya

Menach

Patel

Oburra

2014

International Journal of Otolaryngology

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Background. Laryngeal squamous cell carcinoma is a common head and neck cancer worldwide. Objective. To determine the demographic characteristics of patients with laryngeal cancer, establish their tumor characteristics and relate it to their smoking and alcohol ingestion habits. Methods. Fifty cases and fifty controls were recruited of matching age, sex, and region of residence. History and pattern of cigarette smoking and alcohol ingestion was taken and analyzed. Results. 33 (66%) of the cases and 3 (6%) among controls were current cigarette smokers. 74% had smoked for more than 30 years, P < 0.0001 OR 21.3 (95% CI: 2.6–176.1). There was a male predominance (96%) and most cases (62%) were from the ethnic communities in the highland areas of Kenya predominantly in Central and Eastern provinces. Very heavy drinkers had increased risk of P < 0.0001 OR, 6.0 (95% CI: 1.957–18.398) and those who smoked cigarettes and drank alcohol had poorly differentiated tumors G3, P < 0.001, OR 11.652 (95% CI 2.305–58.895), and G4, P=0.52 OR 7.286 (95% CI 0.726–73.075). They also presented with advanced disease (73.6%). Conclusion. Cigarette smoking and alcohol ingestion are strong risk factors for development of late stage and poorly differentiated laryngeal squamous cell carcinoma in Kenya.

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Cigarette Smoking and Alcohol Ingestion as Risk Factors for Laryngeal Squamous Cell Carcinoma at Kenyatta National Hospital, Kenya

Cited by 22 publications

References 29 publications

LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis

LncRNA MEG3 inhibits cell proliferation and induces apoptosis in laryngeal cancer via miR‐23a/APAF‐1 axis

MicroRNA-93 regulates cyclin G2 expression and plays an oncogenic role in laryngeal squamous cell carcinoma

Demography and Histologic Pattern of Laryngeal Squamous Cell Carcinoma in Kenya

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