Cigarette Smoking and hOGG1 Ser326Cys Polymorphism are Associated with 8-OHdG Accumulation on Mitochondrial DNA in Thoracic Esophageal Squamous Cell Carcinoma

Lin, Ching-Heng; Wang, Liang Shun; Chou, Teh Ying; Hsu, Wen Hu; Lin, Hui; Lee, Shu Yu; Lee, Mau Hua; Chang, Shi Chuan; Wei, Yau‐Huei

doi:10.1245/s10434-012-2576-z

Cited by 18 publications

(18 citation statements)

References 29 publications

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“…On the contrary, an increase in mtDNA copy number was noted in the carcinogenesis of head and neck cancers (36), and the progression of esophageal squamous cell carcinomas (20), particularly in patients who smoked cigarettes. These increases were regarded as a compensation process to overcome the oxidative damages from cigarette smoking and to maintain proper mitochondrial function (19). Concerning BIDCs, a decrease in mtDNA copy number was reported in some research articles (37)(38)(39).…”

Section: Discussionmentioning

confidence: 99%

“…In our preliminary result, advanced T status was associated with a higher incidence of D310 mutation and an elevated mtDNA copy ratio. It is reasonable to assume that the increase in mtDNA copy number compensated for the mtDNA with D310 mutations when the BIDCs underwent progression, which was proposed in head and neck cancer and esophageal cancer (19,20,36). To verify whether these increases in mtDNA copy number and mtDNA D310 mutations confer enhanced aggressiveness to BIDCs, further in vitro study is warrant.…”

Section: Mitochondrial Dna D310 Mutation ----------------------------mentioning

confidence: 99%

“…These tissue samples were stored in 1.5-ml Eppendorf vials and mixed with 500 µl xylene (Merck KGaA, Darmstadt, Germany) at room temperature for 16 h. After centrifuging at 10,000 x g for 10 min at room temperature and discarding the supernatants, these tissue samples were re-hydrated with 100, 80, 60 and 40% alcohol aqueous solution and then pure distilled water for 5 min in steps. Finally, the hydrated tissue samples were mixed with 200 µl QuickExtract DNA extraction solution (Epicenter, Madison, WI, uSA) plus 3 µl of 5% butylated hydroxytoluene in methanol to extract total cellular DNA at 65˚C for 3 h as previously described (19,20). The DNA sample was kept at -20˚C until use.…”

Section: Patient Selection Tissue Preparation and Dna Extractionmentioning

confidence: 99%

“…The sequences of primers used for nDNA amplification (18S rRNA region) were: 18SF1546, 5'-T AGAGGGACAAGTGGCGTTC-3'; and 18SR1650, 5'-CGCT GAGCCAGTCAGTGT-3' (21). The equations of standard curves set for mtDNA and nDNA quantification were set as previously described (19,20,22). Then the mtDNA and nDNA copies of the clinical samples relative to mtDNA and nDNA copies of the 143B osteosarcoma cells were calculated.…”

Section: Standard Curves For Mtdna and Ndna Quantificationsmentioning

confidence: 99%

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Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Lin¹,

Chang²,

Ou³

et al. 2015

Oncology Reports

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Abstract. We analyzed the changes in mitochondrial DNA (mtDNA) copy numbers and the shifting of mtDNA D310 sequence variations (D310 mutation) with their relationships to pathological status and the expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER-2/neu), tumor-suppressor protein p53 and cellular proliferation protein Ki-67 in breast invasive ductal carcinoma (BIDC), respectively. Fifty-one paraffin-embedded BIDCs and their paired non-cancerous breast tissues were dissected for DNA extraction. The mtDNA copy number and mtDNA D310 sequence variations were determined by quantitative real-time polymerase chain reaction (q-PCR) and PCR-based direct sequencing, respectively. The expression levels of ER, PR, HER-2/neu, p53 and Ki-67 were determined by immunohistochemical (IHC) staining. Compared to the paired non-cancerous breast tissues, 24 (47.1%) BIDCs had elevated mtDNA copy numbers and 29 (56.9%) harbored mtDNA D310 mutations. Advanced T-status (p=0.056), negative-ER (p=0.005), negative-PR (p=0.007), positive-p53 (p=0.050) and higher Ki-67 (p=0.004) expressions were related to a higher mtDNA copy ratio. In addition, advanced T-status (p=0.019) and negative-HER-2/neu expression (p=0.061) were associated with mtDNA D310 mutations. In conclusion, higher mtDNA copy ratio and D310 mutations may be relevant biomarkers correlated with pathological T-status and the expression levels of ER, PR, HER-2/neu, p53 and Ki-67 in BIDCs.

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Section: Discussionmentioning

confidence: 99%

Section: Mitochondrial Dna D310 Mutation ----------------------------mentioning

confidence: 99%

Section: Patient Selection Tissue Preparation and Dna Extractionmentioning

confidence: 99%

Section: Standard Curves For Mtdna and Ndna Quantificationsmentioning

confidence: 99%

See 2 more Smart Citations

Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Lin¹,

Chang²,

Ou³

et al. 2015

Oncology Reports

Self Cite

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“…A detailed protocol has been published elsewhere. 24 In brief, we adapted a multiplex real-time polymerase chain reaction (RT-PCR) to measure mtDNA content. 25 We quantified mtDNA copy number using mtDNA 12S ribosomal RNA TaqMan probe (6FAM- 5′ TGCCAGCCACCGCG 3′-MGB) (Applied Biosystems, Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

Associations of Annual Ambient Fine Particulate Matter Mass and Components with Mitochondrial DNA Abundance

et al. 2017

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Background Fine particulate matter (PM2.5) represents a mixture of components with potentially different toxicities. However, little is known about the relative effects of PM2.5 mass and PM2.5 components on mitochondrial DNA (mtDNA) abundance, which may lie on the pathway of PM2.5-associated disease. Methods We studied 646 elderly male participants in the Normative Aging Study from Greater Boston to investigate associations of long-term exposure to PM2.5 mass and PM2.5 components with mtDNA abundance. We estimated concentrations of pollutants for the 365-day preceding examination at each participant's address using spatial-and-temporal-resolved chemical transport models. We measured blood mtDNA abundance using RT-PCR. We applied a shrinkage-and-selection method (adaptive-LASSO) to identify components most predictive of mtDNA abundance, and fit multi-pollutant linear mixed-effects models with subject-specific intercept to estimate the relative effects of individual PM component. Results MtDNA abundance was negatively associated with PM2.5 mass in the previous year and—after adjusting for PM2.5 mass—several PM2.5 components, including organic carbon, sulfate (marginally) and nitrate. In multi-pollutant models including as independent variables PM2.5 mass and PM2.5 components selected by LASSO, nitrate was associated with mtDNA abundance. A standard deviation (SD) increase in annual PM2.5-associated nitrate was associated with a 0.12 SD (95% CI: -0.18; -0.07) decrease in mtDNA abundance. Analyses restricted to PM2.5 annual concentration below the current 1-year EPA standard produced similar results. Conclusions Long-term exposures to PM2.5-associated nitrate were related to decreased mtDNA abundance independent of PM2.5 mass. Mass alone may not fully capture the potential of PM2.5 to oxidize the mitochondrial genome.

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A systematic review and meta-analysis of the association between OGG1 Ser326Cys polymorphism and cancers

Zhou

et al. 2015

Med Oncol

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The oxyguanine glycosylase 1 (OGG1) gene has an important role in DNA repair, and the polymorphism of the gene may alter cancer susceptibility. This study aims to examine the association between the OGG1 Ser326Cys polymorphism and cancer risk based on meta-analysis. Relevant studies were identified through a search of PubMed and Weipu databases, and a total of 109 studies including 111 comparisons containing 34,041 cases and 42,730 controls were enrolled. Overall, significant association was observed between OGG1 Ser326Cys polymorphism and cancer risk in all genetic models except for heterozygote model (Cys/Cys + Cys/Ser vs Ser/Ser: OR 1.071, 95 % CI 1.019-1.125; Cys/Cys vs Cys/Ser + Ser/Ser: OR 1.159, 95 % CI 1.076-1.248; Cys/Cys vs Ser/Ser: OR 1.202, 95 % CI 1.105-1.308). In stratified analysis by cancer type, significantly increased cancer risk was observed in digestive system cancer, head and neck cancer and lung cancer. For gynecologic cancer, significantly increased cancer risk was also observed in homozygote model (OR 1.974, 95 % CI 1.254-3.107). In addition, in stratified analysis by ethnicities, increased cancer risk was found in Asians (Cys/Cys vs Cys/Ser + Ser/Ser: OR 1.195, 95 % CI 1.088-1.313; Cys/Cys + Cys/Ser vs Ser/Ser: OR 1.115, 95 % CI 1.045-1.190; Cys/Cys vs Ser/Ser: OR 1.273, 95 % CI 1.149-1.410). The OGG1 Ser326Cys polymorphism may be a risk factor for cancers of lung, digestive system and head and neck.

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Cigarette Smoking and hOGG1 Ser326Cys Polymorphism are Associated with 8-OHdG Accumulation on Mitochondrial DNA in Thoracic Esophageal Squamous Cell Carcinoma

Abstract: Elevated 8-OHdG accumulations on mtDNA in TESCC were observed. Such accumulations were associated with a compensatory increase in total mtDNA copy number, indexes of cigarette smoking, and hOGG1 Ser326Cys polymorphisms.

Cited by 18 publications

References 29 publications

Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Mitochondrial DNA alterations correlate with the pathological status and the immunological ER, PR, HER-2/neu, p53 and Ki-67 expression in breast invasive ductal carcinoma

Associations of Annual Ambient Fine Particulate Matter Mass and Components with Mitochondrial DNA Abundance

A systematic review and meta-analysis of the association between OGG1 Ser326Cys polymorphism and cancers

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