Cilia regeneration requires an RNA splicing factor from the ciliary base

Abstract

Cilia are microtubule-based organelles projected from most eukaryotic cell surfaces performing cell motility and signaling. Several previously recognized non-ciliary proteins play crucial roles in cilium formation and function. Here, we provide additional evidence that the Caenorhabditis elegans RNA splicing factor PRP-8/PRPF8 regulates ciliogenesis and regeneration from the ciliary base. Live imaging of GFP knock-in animals reveals that the endogenous PRP-8 localizes in the nuclei and the ciliary base. A weak loss-of-function allele of prp-8 affects ciliary structure but with little impact on RNA splicing. Conditional degradation of PRP-8 within ciliated sensory neurons showed its direct and specific roles in cilium formation. Notably, the penetrance of ciliary defects correlates with the reduction of PRP-8 at the ciliary base but not nuclei, and sensory neurons regenerated cilia accompanying PRP-8 recovery from the ciliary base rather than the nuclei. We suggest that PRP-8 at the ciliary base contributes to cilium formation and regeneration.

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“…RNA interference (RNAi) was carried out as in (Xu et al, 2022) (Xu and Ou, 2022). Designed target sequence for tbb-1 could be found in (Honda et al, 2017) (Honda et al, 2017), this target was proved to inhibit expression of tbb-1 efficiently.…”

Correction: Cilia regeneration requires an RNA splicing factor from the ciliary base

“…Following publication of the original article(Xu and Ou 2022), it is reported that the supplement figure S1-S5 were missing from the article. Additional file 1: Fig.S1contains only figure legends but no figures.The original article (Xu and Ou 2022) has been corrected.…”

“…Following publication of the original article (Xu and Ou 2022 ), it is reported that the supplement figure S1-S5 were missing from the article. Additional file 1: Fig.…”