Ciliation of bovine aortic endothelial cells in culture

Briffeuil, Pascal; Thibaut-Vercruyssen, R; Ronveaux-Dupal, M.-F.

doi:10.1016/0021-9150(94)90084-1

Cited by 8 publications

(10 citation statements)

References 22 publications

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“…The other microvilli are presumably true microvilli that contain actin filaments. Reports on endothelial cilia describe them as nonmotile primary cilia (Gallagher,1980; Bystrevskaya et al,1988; Briffeuil et al,1994; Iomini et al,2004). Moreover, motile monocilia, propelling fluid, are not to be expected in the flow dominated cardiovascular system.…”

Section: Discussionmentioning

confidence: 99%

“…Bystrevskaya et al (1988) described primary cilia either entirely immersed in the cytoplasm or protruding from the abluminal cell surface in aortas of 22–24 week human fetuses. Briffeuil et al (1994) observed primary cilia on senescent bovine aortic endothelial cells in static culture, protruding from the cell surface. Furthermore, primary cilia were detected on the corneal endothelium (Gallagher,1980).…”

Section: Introductionmentioning

confidence: 99%

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Monocilia on chicken embryonic endocardium in low shear stress areas

Heiden

Groenendijk

Hierck

et al. 2005

Developmental Dynamics

127

114

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During cardiovascular development, fluid shear stress patterns change dramatically due to extensive remodeling. This biomechanical force has been shown to drive gene expression in endothelial cells and, consequently, is considered to play a role in cardiovascular development. The mechanism by which endothelial cells sense shear stress is still unidentified. In this study, we postulate that primary cilia function as fluid shear stress sensors of endothelial cells. Such a function already has been attributed to primary cilia on epithelial cells of the adult kidney and of Hensen's node in the embryo where they transduce mechanical signals into an intracellular Ca 2ϩ signaling response. Recently, primary cilia were observed on human umbilical vein endothelial cells. These primary cilia disassembled when subjected to high shear stress levels. Whereas endocardial-endothelial cells have been reported to be more shear responsive than endothelial cells, cilia are not detected, thus far, on endocardial cells. In the present study, we use field emission scanning electron microscopy to show shear stress-related regional differences in cell protrusions within the cardiovasculature of the developing chicken. Furthermore, we identify one of these cell protrusions as a monocilium with monoclonal antibodies against acetylated and detyrosinated alphatubulin. The distribution pattern of the monocilia was compared to the chicken embryonic expression pattern of the high shear stress marker Krü ppel-like factor-2. We demonstrate the presence of monocilia on endocardial-endothelial cells in areas of low shear stress and postulate that they are immotile primary cilia, which function as fluid shear stress sensors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monocilia on chicken embryonic endocardium in low shear stress areas

Heiden

Groenendijk

Hierck

et al. 2005

Developmental Dynamics

127

114

View full text Add to dashboard Cite

show abstract

“…6) have evidence that the endothelial cells of large blood vessels of rabbit, like the dog, are non-ciliatcd. A recently published report by Briffeuil et al [23] suggests that cultured bovine aortic endothelial cells possess cilia, but they did not mention the state in vivo. Huvec (human umbilical vein endothelial cells) grown in culture in our laboratory have definitely been devoid of cilia.…”

Section: Atherosclerosismentioning

confidence: 99%

“…However, the expression 9 + 0 cilia has been used to augment rather than provide a familiar name. Primary cilia have also been described as 'solitary' [23] or 'rudimentary' [24], while Ghadially [16] prefers the term 'oligocilia'. The last refers to their tendency to occur in small numbers rather than en masse, but does not make for a clear and charac teristic distinction from small groups of 9 + 2 cilia (e.g.…”

Section: + 0 and 'Primary'as An Acceptable Nomenclaturementioning

confidence: 99%

Primary Cilia in Normal and Pathological Tissues

1995

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The medical literature in pathology contains a surprising number of anecdotal reports of primary cilia, with authors often expressing some incredulity at finding such organelles. In this update of an earlier review, I will argue that primary cilia should by now be regarded as regular cell organelles, not some form of atypical response to unusual circumstances. In all situations in which their presence is essential, they unquestionably act as sensory transducers. Detection by electron microscopy has been the most reliable means until recently, but is time-consuming and slow for any systematic investigation or experimental approach. Immunostaining with an antibody directed against detyrosinated tubulin is rapidly changing the situation, and we can now detect their presence, frequency, disposition and overall characteristics relatively quickly, allowing better statistical analysis and correlations in abnormal physiological and pathological conditions. To be useful and meaningful, comparative studies need a reliable database of information about primary cilia under ‘normal’ circumstances. It is hoped that such work will in itself give much further insight into the general significance of these organelles, especially combined with the more experimental approaches that can now be adopted in the study of their development and function, which looks increasingly promising with the new technological improvements.

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“…PC on EC act as fluid shear stress sensors (van der Heiden et al 2006;Nauli et al 2008) and have been visualized in live zebrafish embryos, where they appeared to detect shear forces during vascular morphogenesis (Goetz et al 2014). Cultures of mammalian and avian vascular EC have been shown to maintain PC (Briffeuil et al 1994;van der Heiden et al 2006). Human umbilical vein cells (HUVEC) in culture disassemble their PC in response to high shear stress (Iomini et al 2004) and have become an important in vitro model for studying ciliogenesis and shear stress signalling in the vascular endothelium (Geerts et al 2011).…”

Section: Primary Cilia (Pc)mentioning

confidence: 98%

Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

Bols

2015

Fish Physiol Biochem

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Primary cilia (PC) were demonstrated for the first time in fish endothelial, epithelial and fibroblast cell lines through immunofluorescence staining with the monoclonal antibody, 6-11B-1, against acetylated α-tubulin. The study was carried out with eight recently developed cell lines from the walleye, Sander vitreus (Mitchill). These were three fibroblast-like cell lines, WE-cfin11f, WE-skin11f and WE-liver3 from, respectively, the caudal fin, skin and liver, and three epithelial-like cell lines, WE-cfin11e, WE-spleen6 and WErpe from, respectively, the caudal fin, spleen and retina. Also, endothelial-like WEBA from the bulbus arteriosus and glial-like WE-brain5 from the brain were used. Immunocytochemistry revealed strong staining for acetylated α-tubulin in mitotic spindles and midbodies for all cell lines, and in PC for all cell lines except WE-skin11f. Staining of cytoplasmic microtubules (fibrils) was absent in three cell lines, including WEBA, but present in the others, especially WE-skin11f, which might have obscured PC detection in these cells. Tubacin, an inhibitor of histone deacetylase 6, induced cytoplasmic fibrils in WEBA and the intensity of their staining in WE-cfin11f. These results suggest that the cell lines might differ in their deacetylase activities, making them useful for studying this tubulin modification in teleosts, as well as for studying PC.

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Ciliation of bovine aortic endothelial cells in culture

Cited by 8 publications

References 22 publications

Monocilia on chicken embryonic endocardium in low shear stress areas

Monocilia on chicken embryonic endocardium in low shear stress areas

Primary Cilia in Normal and Pathological Tissues

Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

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