Ciliogenesis in tissue-cultured cells by the increased density of cell population

Mori, Yoichi; Akedo, Hitoshi; Tanigaki, Yumiko; Tanaka, Kazuhiko; Okada, Masako

doi:10.1016/0014-4827(79)90408-7

Cited by 23 publications

(12 citation statements)

References 12 publications

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“…On the other hand, the MTs elongating to the tip of the proplatelet originate most likely from centriole free MTOC remaining in the cell body, naturally in the PCC. Mori et al [39] demonstrated the ciliogenesis in cultured cells, LI10, an established cell line derived from rat liver. They observed that the centrioles migrated from the center of Golgi complex to the apex of the cells at the postconfluent stage.…”

Section: Translocation Of the Centriole During Differentiation Of Thementioning

confidence: 99%

Platelet production by megakaryocytes: protoplatelet theory justifies cytoplasmic fragmentation model

Kōsaki

2008

Int J Hematol

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The maturation of megakaryocytes (MKs) leading to platelet production is carefully reviewed. For instance, when MK with ploidy 16N enters the maturation stage, eight centrosomes are clustered in the cell center surrounded by 16N nucleus. Each bundle of microtubules (MTs) emanated from the respective centrosome supports and organize eight equally volumed cytoplasmic compartments which together compose one single 16N MK. Then, the following three processes take place in parallel until the complete maturation of MKs Virchow's Arch. 1906;186:55-63. Two centrioles, composing centrosome, are separated and each one with pericentriolar material migrates to just beneath the plasma membrane through the MT bundle [corresponding to a half of the interphase array, originated from one centrosome, supporting one "putative cytoplasmic compartment "(PCC)] (Blood. 2005;106:4066-75). Platelet specific granules and other cellular components, newly formed in the central field of the cell, are transported along the MTs and many platelet territories, future platelets, are elaborated as a tandem array from the center to periphery in each PCC [3]. All the important membranes including plasma membrane and platelet demarcation membrane (DM) are synthesized de novo and those are transported as membrane vesicles (MVs) from Golgi body along the MTs. MVs arranged on the boundary surface between neighboring PCCs undergo fusion and fission to yield a paired membrane. Further connection with the external milieu results in the completion of DM system. The PCC covered by a sheet of DM is designated as protoplatelet. Excessive production of the MVs, most probably intervenes between the respective protoplatelets. Eventually, the matured MK ruptures as a whole, resulting in release of platelets from protoplatelets and many of MVs, though the mechanism is not fully elucidated yet.

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Section: Translocation Of the Centriole During Differentiation Of Thementioning

confidence: 99%

Platelet production by megakaryocytes: protoplatelet theory justifies cytoplasmic fragmentation model

Kōsaki

2008

Int J Hematol

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“…Withdrawal from the cell cycle is tightly associated with ciliogenesis in cultured cells, and serum starvation is a commonly used method for inducing ciliogenesis [6][7][8] . Cell confluence, eliciting contact inhibition of cell proliferation, also facilitates ciliogenesis in cultured cells 9 . However, despite the tight correlation, cell cycle exit itself is unlikely to be an absolute prerequisite for ciliogenesis in that actively cycling progenitor cells in mouse embryos display primary cilia and depend on cilium-related signalling 10 .…”

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confidence: 99%

Actin remodelling factors control ciliogenesis by regulating YAP/TAZ activity and vesicle trafficking

et al. 2015

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Primary cilia exert a profound impact on cell signalling and cell cycle progression. Recently, actin cytoskeleton destabilization has been recognized as a dominant inducer of ciliogenesis, but the exact mechanisms regulating ciliogenesis remain poorly understood. Here we show that the actin cytoskeleton remodelling controls ciliogenesis by regulating transcriptional coactivator YAP/TAZ as well as ciliary vesicle trafficking. Cytoplasmic retention of YAP/TAZ correlates with active ciliogenesis either in spatially confined cells or in cells treated with an actin filament destabilizer. Moreover, knockdown of YAP/TAZ is sufficient to induce ciliogenesis, whereas YAP/TAZ hyperactivation suppresses serum starvation-mediated ciliogenesis. We also identify actin remodelling factors LIMK2 and TESK1 as key players in the ciliogenesis control network in which YAP/TAZ and directional vesicle trafficking are integral components. Our work provides new insights for understanding the link between actin dynamics and ciliogenesis.

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“…Several cases have been reported of malignant cell types apparently producing cilia after malignant transfor mation when their 'normal counterparts' were supposedly unciliated, as in the case of hcpatocytes of the LI-10 line grown in vitro [40], To this unusual example must be add ed the equally remarkable demonstration by Ohtsuki et al [41 ] that two human myeloid cell lines, which are normal ly non-ciliated, expressed primary cilia when transplanted into hamsters. Is this an example of a kind of atavistic tendency associated with the 'dedifferentiation' of cells following malignant transformation in the latter case, or due to their being placed in the less restrained environ ment of cell culture in the former?…”

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confidence: 99%

Primary Cilia in Normal and Pathological Tissues

1995

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The medical literature in pathology contains a surprising number of anecdotal reports of primary cilia, with authors often expressing some incredulity at finding such organelles. In this update of an earlier review, I will argue that primary cilia should by now be regarded as regular cell organelles, not some form of atypical response to unusual circumstances. In all situations in which their presence is essential, they unquestionably act as sensory transducers. Detection by electron microscopy has been the most reliable means until recently, but is time-consuming and slow for any systematic investigation or experimental approach. Immunostaining with an antibody directed against detyrosinated tubulin is rapidly changing the situation, and we can now detect their presence, frequency, disposition and overall characteristics relatively quickly, allowing better statistical analysis and correlations in abnormal physiological and pathological conditions. To be useful and meaningful, comparative studies need a reliable database of information about primary cilia under ‘normal’ circumstances. It is hoped that such work will in itself give much further insight into the general significance of these organelles, especially combined with the more experimental approaches that can now be adopted in the study of their development and function, which looks increasingly promising with the new technological improvements.

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Ciliogenesis in tissue-cultured cells by the increased density of cell population

Cited by 23 publications

References 12 publications

Platelet production by megakaryocytes: protoplatelet theory justifies cytoplasmic fragmentation model

Platelet production by megakaryocytes: protoplatelet theory justifies cytoplasmic fragmentation model

Actin remodelling factors control ciliogenesis by regulating YAP/TAZ activity and vesicle trafficking

Primary Cilia in Normal and Pathological Tissues

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