Ciliogenesis membrane dynamics and organization

Zhao, Huijie; Khan, Ziam; Westlake, Christopher J.

doi:10.1016/j.semcdb.2022.03.021

Cited by 43 publications

(35 citation statements)

References 194 publications

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“…Moreover, they suggested that these cilia were stable through both meiotic divisions in dividing spermatocytes with yet unknown functions [50]. Therefore, it seems that, although somatic primary cilia in vertebrates are a sensory machinery during interphase which was considered a “sleeping beauty” to date during cell division [51, 52], cells that undergo meiosis are capable of polymerizing cilia once cell division has started. This may be related to the dynamics of the mitotic versus the meiotic centrosome.…”

Section: Discussionmentioning

confidence: 99%

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

López-Jiménez

Pérez-Martín

Hidalgo

et al. 2022

Preprint

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Cilia are hair-like projections of the plasma membrane with an inner microtubule skeleton known as axoneme. Motile cilia and flagella beat to displace extracellular fluids, playing important roles in the airways and reproductive system, among others. Instead, primary cilia function as cell type-dependent sensory organelles, detecting chemical, mechanical or optical signals from the extracellular environment. Cilia dysfunction is associated with genetic diseases called ciliopathies, and with some types of cancer.Cilia have been recently identified in zebrafish gametogenesis as an important regulator of thebouquetconformation and recombination. However, there is very little information about the structure and functions of cilia in mammalian meiosis. Here we describe the presence of cilia in male mouse meiotic cells. These solitary cilia form transiently in 20% of zygotene spermatocytes and reach considerable lengths (up to 15 μm). CEP164 and CETN3 localization studies indicate that these cilia emanate from the mother centriole, prior to centrosome duplication. In addition, the study of telomeric TFR2 suggests that these cilia are not directly related to thebouquetconformation during early male mouse meiosis. Instead, based on TEX14 labeling of intercellular bridges in spermatocyte cysts, we suggest that mouse meiotic cilia may have sensory roles affecting cyst function during prophase I.

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Section: Discussionmentioning

confidence: 99%

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

López-Jiménez

Pérez-Martín

Hidalgo

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, it suggested that these cilia were stable through both meiotic divisions in dividing spermatocytes with yet unknown functions [59]. Therefore, it appears that, although somatic primary cilia in vertebrates are sensory machinery during interphase, which was considered a "sleeping beauty" to date during cell division [60,61], cells that undergo meiosis are capable of polymerizing cilia once cell division has started. This may be related to the dynamics of the mitotic versus the meiotic centrosome.…”

Section: The Meiotic Cilium In Mouse Spermatogenesismentioning

confidence: 99%

The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication

López-Jiménez

Pérez-Martín

Hidalgo

et al. 2022

Cells

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Cilia are hair-like projections of the plasma membrane with an inner microtubule skeleton known as axoneme. Motile cilia and flagella beat to displace extracellular fluids, playing important roles in the airways and reproductive system. On the contrary, primary cilia function as cell-type-dependent sensory organelles, detecting chemical, mechanical, or optical signals from the extracellular environment. Cilia dysfunction is associated with genetic diseases called ciliopathies and with some types of cancer. Cilia have been recently identified in zebrafish gametogenesis as an important regulator of bouquet conformation and recombination. However, there is little information about the structure and functions of cilia in mammalian meiosis. Here we describe the presence of cilia in male mouse meiotic cells. These solitary cilia formed transiently in 20% of zygotene spermatocytes and reached considerable lengths (up to 15–23 µm). CEP164 and CETN3 localization studies indicated that these cilia emanate from the mother centriole prior to centrosome duplication. In addition, the study of telomeric TFR2 suggested that cilia are not directly related to the bouquet conformation during early male mouse meiosis. Instead, based on TEX14 labeling of intercellular bridges in spermatocyte cysts, we suggest that mouse meiotic cilia may have sensory roles affecting cyst function during prophase I.

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“…Lists of genes associated with specific centrosomal/ciliary functional modules and their presence or otherwise within the ciliary cluster, compiled from recent reviews and primary research publications: Centriolar and PCM components (Jana, 2021); distal and sub-distal appendages (Tischer et al, 2021); transition zone (Garcia-Gonzalo and Reiter, 2017), IFT kinesin and IFT-A/B (Klena and Pigino, 2022), IFT dynein, inner and outer dynein arms, DNAAFs and MIPs (Braschi et al, 2022), the BBSome (Wingfield et al, 2018), ciliary membrane trafficking (Zhao et al, 2023), the N-DRC (Bower et al, 2013), radial spokes 1 and 2 (Gui et al, 2021), radial spoke 3 (Bazan et al, 2021;Urbanska et al, 2015), central apparatus (Gui et al, 2022), and centriolar satellites (Prosser and Pelletier, 2020). (C)…”

Section: Supplemental Tablesmentioning

confidence: 99%

“…Cildb V3.0 number of ciliary evidences (Arnaiz et al, 2014) were collected for each human gene from http://cildb.i2bc.paris-saclay.fr. ( B ) Lists of genes associated with specific centrosomal/ciliary functional modules and their presence or otherwise within the ciliary cluster, compiled from recent reviews and primary research publications: Centriolar and PCM components (Jana, 2021); distal and sub-distal appendages (Tischer et al, 2021); transition zone (Garcia-Gonzalo and Reiter, 2017), IFT kinesin and IFT-A/B (Klena and Pigino, 2022), IFT dynein, inner and outer dynein arms, DNAAFs and MIPs (Braschi et al, 2022), the BBSome (Wingfield et al, 2018), ciliary membrane trafficking (Zhao et al, 2023), the N-DRC (Bower et al, 2013), radial spokes 1 and 2 (Gui et al, 2021), radial spoke 3 (Bazan et al, 2021; Urbanska et al, 2015), central apparatus (Gui et al, 2022), and centriolar satellites (Prosser and Pelletier, 2020). ( C ) Comparison of ciliary cluster with the original comparative analyses performed in 2004 (Avidor-Reiss et al ., 2004; Li et al ., 2004) and the output of the more recent phylogenetic profiling studies (Dey et al ., 2015; Li et al ., 2014; Nevers et al ., 2017).…”

Section: Supplemental Tablesmentioning

confidence: 99%

A Phylogenetic Profiling Approach Identifies Novel Ciliogenesis Genes InDrosophilaAndC. elegans

Dobbelaere

Érdi

et al. 2022

Preprint

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Cilia are cellular projections that perform sensory and motile functions in eukaryotic cells. A defining feature of cilia is that they are evolutionarily ancient yet not universally conserved. In this study we have used the resulting presence and absence pattern in the genomes of diverse eukaryotes to identify a set of 386 human genes associated with cilium assembly or motility. Comprehensive tissue-specific RNAi in Drosophila and mutant analysis in C. elegans revealed signature ciliary defects for 70-80% of novel genes, a percentage similar to that for known genes within the cluster. Further characterization identified different phenotypic classes, including a set of genes related to the cartwheel component Bld10/Cep135 and two highly conserved regulators of cilium biogenesis. We believe this dataset to define the core set of genes required for cilium assembly and motility across eukaryotes, an invaluable resource for future studies of cilium biology and associated disorders.

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Ciliogenesis membrane dynamics and organization

Cited by 43 publications

References 194 publications

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication

A Phylogenetic Profiling Approach Identifies Novel Ciliogenesis Genes InDrosophilaAndC. elegans

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