Cilostazol, a selective Type III phosphodiesterase inhibitor: prevention of cervical myelopathy in a rat chronic compression model

Yamamoto, Shinji; Kurokawa, Ryu; Kim, Phyo

doi:10.3171/2013.9.spine121136

Cited by 28 publications

(35 citation statements)

References 41 publications

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“…16,17 However, the studies did not achieve treatment effects on the progression of compression myelopathy. The present study demonstrated that G-CSF could have promising potential as a treatment to restore developing compression myelopathy.…”

Section: Discussionmentioning

confidence: 99%

“…The rotation speed was set at 10 rpm based on our previous reports. 2,16,17 Before surgery, all rats could walk continuously for more than 300 seconds. Therefore, we set a cut-off of 300 seconds, and latency to fall off the rotarod was measured before and after surgery.…”

Section: Rotarod Performancementioning

confidence: 99%

“…The C5-6 segment of the spinal cord was dissected from each animal, embedded in paraffin, and sectioned at a slice thickness of 5 µm and a gap interval of 5 µm over a 1-mm length, based on stereological considerations of the motor neurons. 2,16,17 One hundred histological sections were analyzed from the C5-6 cord segment for each rat. The specimens were stained with hematoxylin and eosin, and motor neurons in the anterior horn gray matter were counted.…”

Section: Motor Neuron Countingmentioning

confidence: 99%

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Granulocyte Colony-Stimulating Factor Improves Motor Function in Rats Developing Compression Myelopathy

Yoshizumi

Murata

Yamamoto

et al. 2016

Spine

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Section: Discussionmentioning

confidence: 99%

Section: Rotarod Performancementioning

confidence: 99%

Section: Motor Neuron Countingmentioning

confidence: 99%

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Granulocyte Colony-Stimulating Factor Improves Motor Function in Rats Developing Compression Myelopathy

Yoshizumi

Murata

Yamamoto

et al. 2016

Spine

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“…Existing models mimicking chronic cord compression in rats include insertion of expandable polymers [50], adjustable screws [33], and calcification-inducing polymers [28]. These result in loss of neurons, predominantly in the grey matter of the ventral horns at the lesion epicentre.…”

Section: Introductionmentioning

confidence: 99%

“…Preferential neuronal loss has also been demonstrated in canine screw compression models [21] and twy-twy mouse models [1]. In addition, demyelination has been observed in some CSM models [28, 51], although myelin pallor or frank myelin loss is not generally present in expandable polymer animal models [30, 50]. …”

Section: Introductionmentioning

confidence: 99%

Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy

Dhillon

Parker

Syed

et al. 2016

acta neuropathol commun

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Cervical spondylotic myelopathy (CSM) is the most common spinal cord disorder and a major cause of disability in adults. Improvements following surgical decompression are limited and patients often remain severely disabled. Post mortem studies indicate that CSM is associated with profound axonal loss. However, our understanding of the pathophysiology of CSM remains limited.To investigate the hypothesis that axonal plasticity plays a role in the recovery following surgical decompression, we adopted a novel preclinical model of mild to moderate CSM. Spinal cord compression resulted in significant locomotor deterioration, increased expression of the axonal injury marker APP, and loss of serotonergic fibres. Surgical decompression partially reversed the deficits and attenuated APP expression. Decompression was also associated with axonal sprouting, reflected in the restoration of serotonergic fibres and an increase of GAP43 expression. The re-expression of synaptophysin indicated the restoration of functional synapses following decompression. Promoting axonal plasticity may therefore be a therapeutic strategy for promoting neurological recovery in CSM.Electronic supplementary materialThe online version of this article (doi:10.1186/s40478-016-0359-7) contains supplementary material, which is available to authorized users.

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A sheep model of chronic cervical compressive myelopathy via an implantable wireless compression device

et al. 2022

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Purpose This study aimed to establish an animal model in which we can precisely displace the spinal cord and therefore mimic the chronic spinal compression of cervical spondylotic myelopathy. Methods In vivo intervertebral compression devices (IVCDs) connected with subcutaneous control modules (SCCMs) were implanted into the C2-3 intervertebral disk spaces of sheep and connected by Bluetooth to an in vitro control system. Sixteen sheep were divided into four groups: (Group A) control; (Group B) 10-week progressive compression, then held; (Group C) 20-week progressive compression, then held; and (Group D) 20-week progressive compression, then decompression. Electrophysiological analysis (latency and amplitude of the N1-P1-N2 wave in somatosensory evoked potentials, SEP), behavioral changes (Tarlov score), imaging test (encroachment ratio (ER) of intraspinal invasion determined by X-ray and CT scan), and histological examinations (hematoxylin and eosin, Nissl, and TUNEL staining) were performed to assess the efficacy of our model. Results Tarlov scores gradually decreased as compression increased with time and partially recovered after decompression. The Pearson correlation coefficient between ER and time was r = 0.993 (p < 0.001) in Group B at 10 weeks and Groups C and D at 20 weeks. And ER was negatively correlated with the Tarlov score (r = -0.878, p < 0.001). As compression progressed, the SEP latency was significantly extended (p < 0.001), and the amplitude significantly decreased (p < 0.001), while they were both partially restored after decompression. The number of abnormal motor neurons and TUNEL-positive cells increased significantly (p < 0.001) with compression. Conclusion Our implantable and wireless intervertebral compression model demonstrated outstanding controllability and reproducibility in simulating chronic cervical spinal cord compression in animals.

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Cilostazol, a selective Type III phosphodiesterase inhibitor: prevention of cervical myelopathy in a rat chronic compression model

Abstract: These results indicate that cilostazol is neuroprotective in the chronically compressed cervical cord and is potentially useful in the treatment of cervical spondylotic myelopathy.

Cited by 28 publications

References 41 publications

Granulocyte Colony-Stimulating Factor Improves Motor Function in Rats Developing Compression Myelopathy

Granulocyte Colony-Stimulating Factor Improves Motor Function in Rats Developing Compression Myelopathy

Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy

A sheep model of chronic cervical compressive myelopathy via an implantable wireless compression device

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