Cilostazol alleviates streptozotocin-induced testicular injury in rats via PI3K/Akt pathway

Mohamed, Mervat Z.; Hafez, Heba M.; Zenhom, Nagwa M.; Mohammed, Hanaa Hassanein

doi:10.1016/j.lfs.2018.02.038

Cited by 33 publications

(24 citation statements)

References 39 publications

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“…Earlier reports have suggested that hyperglycemia induced oxidative stress can initiate several inflammatory reactions and pathways, a vital predisposing factor for diabetic induced testicular dysfunction. Proinflammatory biomarkers including IL‐6 and TNF‐α have been reported to be high in the testes tissues of diabetic rats 34,41,55 . TNF‐α, IL‐6 and IL‐1β have been implicated in the modulation of the hypothalamic‐pituitary‐testes axis, through their direct effect on gonadotropin‐releasing hormone, FSH and LH.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of ethyl acetate extract from Shorea roxburghii against diabetes induced testicular damage in rats

Zhao

Olatunji

2020

Environmental Toxicology

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Diabetic mellitus is a chronic metabolic disorder that is associated with several complications including testicular dysfunction. This research investigated the protective action of the ethyl acetate extract from Shorea roxburghii (SRE) on diabetes induced testicular damage in rats. Diabetic rats were orally administered with SRE at doses of 100 and 400 mg/kg for 4 weeks. SRE improved the body weight gain, testes weight, testes index and increased serum concentration of testosterone. Furthermore, SRE increased the testicular antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase. In addition, SRE ameliorated testicular inflammatory mediators such as myeloperoxidase, tumor necrosis factor alpha, interleukin 6, p38 MAPK and nuclear factor kappa B activation and decreased testicular cell apoptosis in the treated diabetic rats. SRE also raised sperm parameters after treatment of diabetic rats. Conclusively, our results suggested that SRE ameliorated diabetes induced testicular damage by inhibiting oxidative stress and inflammation.

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Section: Discussionmentioning

confidence: 99%

Protective effects of ethyl acetate extract from Shorea roxburghii against diabetes induced testicular damage in rats

Zhao

Olatunji

2020

Environmental Toxicology

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“…The sets of primers used were given in Table 1. [23][24][25] The qRT-PCR results were analyzed using the DDCt method with normalization to the beta-actin as internal control in order to adjust the samples and avoid difference in sample loading. 26 Enzyme-linked immunosorbent assay (ELISA)…”

Section: Histological and Immunohistochemical Examinationmentioning

confidence: 99%

PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats

et al. 2020

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Endometrial hyperplasia (EH) is a medical condition that affects many females as it increases their uterine carcinogenic potential. EH results from entangling hormonal imbalance and inflammatory response. The study examined the role of a xanthine oxidase inhibitor, febuxostat, in a rat model of EH. Adult female Wistar albino rats were subjected to estradiol valerate (EV) 2 mg/kg for 10 days to induce EH. Another group was treated concomitantly with febuxostat 10 mg/kg for the same period. The uterine malondialdehyde, reduced glutathione (GSH), and superoxide dismutase (SOD) were assessed by chemical methods. Gene expressions of phosphatidylinositol-3-kinase (PI3K), Akt, and hypoxia-inducible factor 1 alpha were assessed by the quantitative real-time polymerase chain reaction. Moreover, the vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay. Histopathology and immunohistochemical techniques were used for the detection of phosphatase and tensin homolog (PTEN). The results revealed that EV administration induced complex EH with focal atypia and loss of PTEN expression by the histological examination. Uteri of the EV group showed a significant drop in GSH content and SOD activity and rise in the expressions of PI3K, Akt, VEGF, and IL-6. Febuxostat administration significantly improved the uterine GSH and SOD levels. It decreased the expressions of PI3K, Akt, VEGF, and IL-6. The endometrium showed a regression of the proliferative epithelium with the restoration of PTEN expression and the absence of the atypical features. In conclusion, febuxostat protected the endometrium against estrogen-induced EH and may be beneficial in the management along with the hormonal therapy.

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“…27 Despite the role of PI3K/ Akt in inflammation is controversial, 26 our findings in line with others suggested the inhibitory effect of this pathway on inflammation in the liver. [28][29][30] Verapamil achieved decreased NO content together with upregulation of PI3K/Akt gene expression in hepatic tissue.…”

Section: Discussionmentioning

confidence: 99%

PI3K/Akt and Nrf2/HO-1 pathways involved in the hepatoprotective effect of verapamil against thioacetamide toxicity in rats

et al. 2018

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Liver is a precious organ to maintain body life. Hepatotoxicity is a worldwide health problem that is still a challenge for research. Although countless pharmaceutical drugs and herbal compounds were screened for their hepatoprotective effects, the death from hepatotoxicity is increasing. Thus, there is continuous necessity of searching for the hepatoprotective effect of commonly used drugs. Accordingly, our aim was to examine a hepatoprotective potential for the antihypertensive drug, verapamil, and searching for new insights underlie its protective mechanism. Four groups of adult male rats were randomly arranged as controls, thioacetamide (TAA) hepatotoxic, and TAA + verapamil treated. Serum liver enzyme, hepatic antioxidant, lipid peroxidation, and inflammatory parameters were assessed. Gene relative expression for heme oxygenase-1 (HO-1), nuclear factor-erythroid 2-related factor 2 (Nrf2), phosphoinositide 3-kinase (PI3K), and serine/threonine-specific protein kinase (Akt) were quantified in hepatic tissue. TAA caused hepatic injury evident both histopathologically and biochemically by a decrease in all gene expressions. Verapamil alleviated the injury via its antioxidant and anti-inflammatory effects that were suggested to be via upregulation of the previous gene expressions. In conclusion, the calcium channel blocker, verapamil, that is used widely as antihypertensive exhibits a valuable hepatoprotective effect. The protection partially rests on activation of Nrf2/HO-1 and PI3K/Akt pathways.

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Cilostazol alleviates streptozotocin-induced testicular injury in rats via PI3K/Akt pathway

Cited by 33 publications

References 39 publications

Protective effects of ethyl acetate extract from Shorea roxburghii against diabetes induced testicular damage in rats

Protective effects of ethyl acetate extract from Shorea roxburghii against diabetes induced testicular damage in rats

PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats

PI3K/Akt and Nrf2/HO-1 pathways involved in the hepatoprotective effect of verapamil against thioacetamide toxicity in rats

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