Çin’de Ekonomik Öneme Sahip Tavukçuluk İşletmelerinde Endojen Retrovirüs Elemanlarının Genomik Analizi

Gu, Yajia; Li, Tuo-fan; Liang, Xiongyan; Fang, Shouguo; Yang, Yu-ying

doi:10.9775/kvfd.2016.16356

Cited by 1 publication

(2 citation statements)

References 12 publications

(23 reference statements)

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“…The genomic DNA were extracted from chicken embryo fibroblasts as previously described [18] . Briefly, 10-day-old embryonated chicken eggs were minced and treated with trypsin, then the genomic DNA were extracted with Genomic DNA Extraction Kit (GK0222, Generay Biotech.…”

Section: Dna Extractionmentioning

confidence: 99%

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Çin’de Tavuk Cinsleri Arasında Avian Endojen Retrovirus ev/J Genomunda Yeni Delesyonların Tespiti

Liang¹,

Liu²,

Fang³

et al. 2018

Kafkas Univ Vet Fak Derg

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Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression, which has made huge financial loss in the poultry industry. More and more new pathogenic avian leukosis virus, which were recombined from exogenous avian leukosis virus and endogenous retrovirus viruses were isolated from China and other regions in the world. To uncover the characteristic of the potential recombinant elements, we analyzed the genomes of avian endogenous retrovirus ev/J in ten chicken breeds in China. Six of the ten chicken breeds contained two sizes of ev/J (3.8 kb and 2.2 kb) and the other only contained 3.8 kb ev/J. The ten 3.8 kb ev/J were much closer to type I, II and III ev/J prototypes, while the six 2.2 kb ev/J were close to type IV ev/J prototype in the phylogenetic trees. Moreover, three novel deletion fragments were identified in the sixteen ev/J in chicken flocks in China, which made most of the ev/J (10/16) significantly different from the four ev/J prototypes. The emergence of these novel deletions resulted in the diversity of ev/J in chicken breeds in China, which may become the source of further recombination of avian leukosis viruses (ALVs), especially the exogenous ALV subgroup J.

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Section: Dna Extractionmentioning

confidence: 99%

“…We previously reported the prevalence of endogenous retrovirus elements in chicken flocks in China [18] . But the molecular characteristics of ev/J in the chicken breeds in China remained unclear.…”

Section: Introductionmentioning

confidence: 99%