Cinnamic acid 4-hydroxylase from cell cultures of the hornwort Anthoceros agrestis

Petersen, Maike

doi:10.1007/s00425-002-0960-9

Cited by 19 publications

(14 citation statements)

References 16 publications

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“…The temperature optimum of the C4H reaction is generally in the range of 20-30 °C, which is also the case for Pp_AaC4H and Pp_WT with 25 °C. Both values of the heterologously expressed AaC4H correspond to the results for C4H measured in microsome preparations from Anthoceros agrestis suspension cultures (Petersen 2003). The corresponding values for C4H from the liverworts Plagiochasma appendiculatum and Marchantia paleacea were at 30 °C and pH 7.0 (Liu et al 2017).…”

Section: Discussionsupporting

confidence: 77%

“…Only three liverwort genes encoding C4H from Marchantia paleacea and Plagiochasma appendiculatum have been isolated and functionally expressed in yeast and characterized in yeast (Liu et al 2017). C4H from the suspension-cultured hornwort Anthoceros agrestis has been characterized biochemically by Petersen (2003). We here report the isolation of the C4H cDNA from the same hornwort as well as the heterologous expression of AaC4H in the moss Physcomitrella patens.…”

Section: Discussionmentioning

confidence: 99%

“…Cell suspension cultures of Anthoceros agrestis were cultivated as described previously (Petersen 2003).…”

Section: Plant Cell Culturesmentioning

confidence: 99%

“…It catalyzes the hydroxylation (with NADPH and O 2 as cosubstrates) of the aromatic ring of t-cinnamic acid in para-position leading to 4-coumaric acid (4-hydroxycinnamic acid). Petersen (2003) characterized C4H from cell cultures of the hornwort Anthoceros agrestis Paton (Anthocerotaceae) as one of the first cytochrome P450 enzymes from lower plants. Suspension-cultured cells of Anthoceros agrestis can accumulate around 5% of the dry weight as rosmarinic acid (Vogelsang et al 2006), a caffeic acid ester of 3-(3,4-dihydroxyphenyl)lactic acid.…”

Section: Introductionmentioning

confidence: 99%

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Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens

Wohl

Petersen

2020

Plant Cell Rep

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Key message Cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis (AaC4H) was functionally expressed in the moss Physcomitrella patens and characterized at biochemical and molecular levels. Abstract Cinnamic acid 4-hydroxylase (C4H), a cytochrome P450-dependent hydroxylase, catalyzes the formation of 4-coumaric acid (=4-hydroxycinnamic acid) from trans-cinnamic acid. In the hornwort Anthoceros agrestis (Aa), this enzyme is supposed to be involved in the biosynthesis of rosmarinic acid (a caffeic acid ester of 3-(3,4-dihydroxyphenyl)lactic acid) and other related compounds. The coding sequence of AaC4H (CYP73A260) was expressed in the moss Physcomitrella patens (Pp_AaC4H). Protein extracts from the transformed moss showed considerably increased C4H activity driven by NADPH:cytochrome P450 reductase of the moss. Since Physcomitrella has own putative cinnamic acid 4-hydroxylases, enzyme characterization was carried out in parallel with the untransformed Physcomitrella wild type (Pp_WT). Apparent K m-values for cinnamic acid and NADPH were determined to be at 17.3 µM and 88.0 µM for Pp_AaC4H and 25.1 µM and 92.3 µM for Pp_WT, respectively. Expression levels of AaC4H as well as two Physcomitrella patens C4H isoforms were analyzed by quantitative real-time PCR. While PpC4H_1 displayed constantly low levels of expression during the whole 21-day culture period, AaC4H and PpC4H_2 increased their expression during the first 6-8 days of the culture period and then decreased again. This work describes the biochemical in vitro characterization of a cytochrome P450-dependent enzyme, namely C4H, heterologously expressed in the haploid model plant Physcomitrella patens.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

“…Cell suspension cultures of Anthoceros agrestis were cultivated as described previously (Petersen 2003).…”

Section: Plant Cell Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens

Wohl

Petersen

2020

Plant Cell Rep

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“…The possible mechanism at enzyme level behind the observed blue light response may be related to hydroxylation activity of phenolic precursors. Many studies have shown that blue light increases hydroxylase activity, or relaxes inhibition of hydroxylases [38][39][40][41].…”

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confidence: 99%

Blue Mood for Superfood

Taulavuori

Julkunen‐Tiitto

Hyöky

et al. 2013

Natural Product Communications

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The term superfood refers to food with high levels of either nutrient or bioactive phytochemicals with human health benefits. Phytochemicals are naturally occurring compounds in plants that provide their color, flavor and odor. Phenolic compounds form the major constituents of phytochemicals. Plant traits in phytochemical production are tightly bound with the genome while modified markedly by the environmental conditions. Here, we studied the effect of supplemented blue light on the production of several phenolics in the leaves of tomato, basil and parsley, which are widely cultivated food plant species. The results indicated doubled or higher increases in the accumulation of several species-specific phenolic acids or flavonoids. In conclusion, we showed for the first time, that supplemented blue light results in high yield improvement of phytochemicals related to superfood products.

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Rosmarinic Acid and Related Metabolites

Pezeshki

Petersen

2017

Biotechnology of Natural Products

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Cinnamic acid 4-hydroxylase from cell cultures of the hornwort Anthoceros agrestis

Cited by 19 publications

References 16 publications

Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens

Functional expression and characterization of cinnamic acid 4-hydroxylase from the hornwort Anthoceros agrestis in Physcomitrella patens

Blue Mood for Superfood

Rosmarinic Acid and Related Metabolites

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