Cinnamon  bark oil and its components inhibit biofilm formation and toxin production

Kim, Yong‐Guy; Lee, Jin Hyung; Kim, Soon Il; Baek, Kwang-Hyun

doi:10.1016/j.ijfoodmicro.2014.11.028

Cited by 161 publications

(117 citation statements)

References 63 publications

Supporting

Mentioning

105

Contrasting

Unclassified

Order By: Relevance

“…No growth reduction of EHEC cells above 30% at OD 620 was observed at 0.005% (v/v) as compared with untreated controls. Kim et al found that cinnamon bark oil18 had antibiofilm activity against EHEC, but this is the first time that bay, clove, and pimento berry oils have been reported to have antibiofilm activity. In the present study, more detailed study showed bay, clove, and pimento berry oil all dose-dependently inhibited EHEC biofilm formation in 96-well polystyrene plates (Fig.…”

Section: Resultsmentioning

confidence: 92%

“…However, traditional antimicrobial agents primarily designed to inhibit cell growth often result in bacterial drug resistance, and thus, essential oils have been studied using other developmental approaches, such as, the inhibition of biofilm formation, toxin production, bacterial quorum sensing, and of adhesive factors. For example, carvacrol and eugenol14, grapefruit limonoids15, β -sitosterol glucoside from clementine peel16, ginkgolic acids from Ginkgo biloba 17, and cinnamaldehyde and eugenol from cinnamon bark oil18, which are found in essential oils, have been reported to inhibit EHEC biofilm formation. However, few studies have been undertaken to compare the antibiofilm characteristics of large numbers of essential oils.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Essential Oils and Eugenols Inhibit Biofilm Formation and the Virulence of Escherichia coli O157:H7

Kim

Lee

Gwon

et al. 2016

Sci Rep

Self Cite

157

103

View full text Add to dashboard Cite

Enterohemorrhagic Escherichia coli O157:H7 (EHEC) has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms. We investigated the abilities of various plant essential oils and their components to inhibit biofilm formation by EHEC. Bay, clove, pimento berry oils and their major common constituent eugenol at 0.005% (v/v) were found to markedly inhibit EHEC biofilm formation without affecting planktonic cell growth. In addition, three other eugenol derivatives isoeugenol, 2-methoxy-4-propylphenol, and 4-ethylguaiacol had antibiofilm activity, indicating that the C-1 hydroxyl unit, the C-2 methoxy unit, and C-4 alkyl or alkane chain on the benzene ring of eugenol play important roles in antibiofilm activity. Interestingly, these essential oils and eugenol did not inhibit biofilm formation by three laboratory E. coli K-12 strains that reduced curli fimbriae production. Transcriptional analysis showed that eugenol down-regulated 17 of 28 genes analysed, including curli genes (csgABDFG), type I fimbriae genes (fimCDH) and ler-controlled toxin genes (espD, escJ, escR, and tir), which are required for biofilm formation and the attachment and effacement phenotype. In addition, biocompatible poly(lactic-co-glycolic acid) coatings containing clove oil or eugenol exhibited efficient biofilm inhibition on solid surfaces. In a Caenorhabditis elegans nematode model, clove oil and eugenol attenuated the virulence of EHEC.

show abstract

Section: Resultsmentioning

confidence: 92%

mentioning

confidence: 99%

Essential Oils and Eugenols Inhibit Biofilm Formation and the Virulence of Escherichia coli O157:H7

Kim

Lee

Gwon

et al. 2016

Sci Rep

Self Cite

157

103

View full text Add to dashboard Cite

show abstract

“…There are many evidences in literature that cinnamic acid derivatives are inhibitors of biofilm formation [47,48,49,50,51]. The most studied derivate is cinnamaldehyde that interacts with quorum sensing system in bacterial biofilms [46,52,53].…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Spectrum of Biological Activities of Novel N-arylcinnamamides

Pospíšilová

Kos

Michnová

et al. 2018

IJMS

View full text Add to dashboard Cite

A series of sixteen ring-substituted N-arylcinnamamides was prepared and characterized. Primary in vitro screening of all the synthesized compounds was performed against Staphylococcus aureus, three methicillin-resistant S. aureus strains, Mycobacterium tuberculosis H37Ra, Fusarium avenaceum, and Bipolaris sorokiniana. Several of the tested compounds showed antistaphylococcal, antitubercular, and antifungal activities comparable with or higher than those of ampicillin, isoniazid, and benomyl. (2E)-N-[3,5-bis(trifluoromethyl)phenyl]-3-phenylprop-2-enamide and (2E)-3-phenyl-N-[3-(trifluoromethyl)phenyl]prop-2-enamide showed the highest activities (MICs = 22.27 and 27.47 µM, respectively) against all four staphylococcal strains and against M. tuberculosis. These compounds showed an activity against biofilm formation of S. aureus ATCC 29213 in concentrations close to MICs and an ability to increase the activity of clinically used antibiotics with different mechanisms of action (vancomycin, ciprofloxacin, and tetracycline). In time-kill studies, a decrease of CFU/mL of >99% after 8 h from the beginning of incubation was observed. (2E)-N-(3,5-Dichlorophenyl)- and (2E)-N-(3,4-dichlorophenyl)-3-phenylprop-2-enamide had a MIC = 27.38 µM against M. tuberculosis, while a significant decrease (22.65%) of mycobacterial cell metabolism determined by the MTT assay was observed for the 3,5-dichlorophenyl derivative. (2E)-N-(3-Fluorophenyl)- and (2E)-N-(3-methylphenyl)-3-phenylprop-2-enamide exhibited MICs = 16.58 and 33.71 µM, respectively, against B. sorokiniana. The screening of the cytotoxicity of the most effective antimicrobial compounds was performed using THP-1 cells, and these chosen compounds did not shown any significant lethal effect. The compounds were also evaluated for their activity related to the inhibition of photosynthetic electron transport (PET) in spinach (Spinacia oleracea L.) chloroplasts. (2E)-N-(3,5-dichlorophenyl)-3-phenylprop-2-enamide (IC50 = 5.1 µM) was the most active PET inhibitor. Compounds with fungicide potency did not show any in vivo toxicity against Nicotiana tabacum var. Samsun. The structure–activity relationships are discussed.

show abstract

“…CNMA acts as a quorum sensing inhibitor and probably interferes with the cellular processes of S. aureus, including swarming motility, and might possibly affect the bacterial dissemination in the host. 30,55 Additionally, the prolonged survival rates of the nematodes shown in Figure 5 indicate that CNMA-GNPs have no significant biological toxicity under a long incubation period. The observed no toxicity of CNMA-GNPs prominently suggested that the silica coating does not allow the biological system of the nematode to interact with nanoparticle core directly, thus resulting less toxicity.…”

Section: Survival Of Infected C Elegansmentioning

confidence: 97%

Development of gold nanoparticles coated with silica containing the antibiofilm drug cinnamaldehyde and their effects on pathogenic bacteria

Ramasamy

Lee

2017

IJN

Self Cite

View full text Add to dashboard Cite

Emerging resistance to antibiotics is a mounting worldwide health concern and increases the need for nonantibiotic strategies to combat infectious diseases caused by bacterial pathogens. In this study, the authors used the antibiofilm activity of the naturally occurring antimicrobial cinnamaldehyde (CNMA) conjugated to the surface of gold nanoparticles (GNPs) to deliver CNMA efficiently and eradicate biofilms of Gram-negative organisms (enterohemorrhagic Escherichia coli O157:H7, and Pseudomonas aeruginosa ), Gram positive (methicillin-sensitive Staphylococcus aureus organisms, and methicillin-resistant Staphylococcus aureus ) bacteria. CNMA-GNPs containing 0.005% (v/v) of CNMA were found to inhibit biofilm formation efficiently. The distributions of nanoparticles in biofilm cells and their biofilm disruption activities, including distorted cell morphology, were determined by transmission electron microscopy. In addition to their antibiofilm activities, CNMA-GNPs attenuated S. aureus virulence and protected Caenorhabditis elegans ( C. elegans ) worms. Here, the authors report the antibiofilm effects of CNMA-GNPs and suggest that they could be used to treat pathogenic bacterial infections in vivo.

show abstract

Cinnamon bark oil and its components inhibit biofilm formation and toxin production

Cited by 161 publications

References 63 publications

Essential Oils and Eugenols Inhibit Biofilm Formation and the Virulence of Escherichia coli O157:H7

Essential Oils and Eugenols Inhibit Biofilm Formation and the Virulence of Escherichia coli O157:H7

Synthesis and Spectrum of Biological Activities of Novel N-arylcinnamamides

Development of gold nanoparticles coated with silica containing the antibiofilm drug cinnamaldehyde and their effects on pathogenic bacteria

Contact Info

Product

Resources

About