Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota

Abstract

BackgroundCinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers. Recently we found cinobufacini could ameliorate dextran sulfate sodium (DSS)-induced colitis in mice, but the underlying mechanism was not fully understood. In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.MethodsC57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water. DSS-treat mice were gavaged daily either with vehicle (water) or cinobufacini (10.0 or 30.0 mg/kg) for 7 days. The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.ResultsOur data indicated that cinobufacini reversed DSS-induced gut dysbiosis and enhanced intestinal barrier integrity. Moreover, changing of some specific microbial groups such as Proteobacteria and Bacteroides was closely correlated with the re-establishment of intestinal equilibrium and the recovery of intestinal function.ConclusionCinobufacini prevents colitis in mice by modifying the composition and function of gut microbiota. The current study provides additional mechanistic insight in the therapeutic effect of cinobufacini treatment and may pave the way for clinical application of cinobufacini in colitis therapy.

Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis

“…The genes in the metabolic pathways, including amino acid metabolism, terpenoids and polyketides, cofactors and vitamins, other amino acids, energy, and glycan biosynthesis metabolisms of the D group, were much lower than those in the N and DGPH groups ( p < 0.05). This could result in a decreasing metabolic rate in colitic mice, and it was also consistent with previous studies. , The genes related to environment information processing (Membrane Transport, Environmental Adatation) in the D group were up-regulated ( p < 0.05). Once the bacteria perceive the surrounding environmental changes, they will respond to these changes via such modulations like inflammatory reactions .…”

“…This could result in a decreasing metabolic rate in colitic mice, and it was also consistent with previous studies. 61,62 The genes related to environment information processing (Membrane Transport, Environmental Adatation) in the D group were upregulated (p < 0.05). Once the bacteria perceive the surrounding environmental changes, they will respond to these changes via such modulations like inflammatory reactions.…”

“…This trend was reversed after the intervention with WSGP, which has also been reported in previous studies. 62,64 In addition, the gut microbiome of the D group was enriched in categories regarding the cell motility, signal transduction, xenobiotics biodegradation, and metabolism. The signaling molecules and interaction, immune, nervous, endocrine, and digestive systems, folding, sorting, and degradation of the N and DGPH groups have been enriched compared to those in the D group.…”

Acacetin Ameliorates Experimental Colitis in Mice via Inhibiting Macrophage Inflammatory Response and Regulating the Composition of Gut Microbiota

“…Some species at the phylum level (e.g., Proteobacteria) could play a role in the pathogenesis of UC, leading to adhesion and invasion of intestinal epithelial cells, disruption of host defense, stimulation of the inflammatory response, alterations in the structure of intestinal microbiota, and ultimately, promote the onset of UC ( Bai et al, 2019 ). Furthermore, Escherichia , which is an IgA-coated bacteria at the genus level, is the dominant pathogen involved in the pathogenesis of UC ( Xu et al, 2018 ).…”

Baicalin Regulates Autophagy to Intervene with Intestinal Acute Graft-Versus-Host Disease via AMPK/Mtor Pathway and Abnormal Intestinal Flora

“…Bacteroidetes [29] , verrucomicrobia [30] and lachnospiraceae [31] would increase after treatment. These above bacteria have a certain inhibitory effect on in ammation, and play an important role in colitis and other intestinal diseases, which is consistent with our research results.Our data indicated that baicalin may reduce lachnospiraceae by reducing the diversity of ora, reducing the relative abundance of Firmicutes, increasing the relative abundance of Bacteroidetes, Proteobacteria and verrucomicrobia_ FCS020.…”