Ciprofloxacin conjugated gold nanorods with pH induced surface charge transformable activities to combat drug resistant bacteria and their biofilms

Yin, Meihui; Qiao, Zhuangzhuang; Yan, Daoping; Yang, Min; Yang, Lijiao; Wan, Xiaohui; Chen, Hualin; Luo, Jianbin; Xiao, Huining

doi:10.1016/j.msec.2021.112292

Cited by 26 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surface-charge-adaptive gold nanoparticles or polymer micelles show good targeting and permeability to bacterial biofilms using the pH difference between the physiological environment and bacterial biofilm. Due to the pH difference between the physiological environment and bacterial biofilm, surface-charge-adaptive gold nanoparticles or polymer micelles show good targeting and permeability to bacterial biofilms. , As proven in our previous study, pH-induced surface-charge-switchable GNRs show full penetration through bacterial biofilms and eradicate mature bacterial biofilms through a chemo-thermotherapy combination.…”

Section: Introductionmentioning

confidence: 56%

“…Due to the pH difference between the physiological environment and bacterial biofilm, surfacecharge-adaptive gold nanoparticles or polymer micelles show good targeting and permeability to bacterial biofilms. 19,20 As proven in our previous study, 21 pH-induced surface-chargeswitchable GNRs show full penetration through bacterial biofilms and eradicate mature bacterial biofilms through a chemo-thermotherapy combination.…”

Section: ■ Introductionmentioning

confidence: 70%

See 1 more Smart Citation

Surface-Charge-Switchable and Size-Transformable Thermosensitive Nanocomposites for Chemo-Photothermal Eradication of Bacterial Biofilms in Vitro and in Vivo

Yin

Yang

Yan

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

The appearance of multidrug-resistant bacteria and their biofilms presents a serious threat to modern medical systems. Herein, we fabricated a novel gold-nanorod-based chemo-photothermal-integrated antimicrobial platform with surface-charge-switchable and near-infrared (NIR)-induced size-transformable activities that show an enhanced killing efficiency against methicillin-resistant Staphylococcus aureus (MRSA) in both planktonic and biofilm phenotypes. The nanocomposites are prepared by in situ copolymerization using N-isopropyl acrylamide (NIPAM), acrylic acid (AA), and N-allylmethylamine (MAA) as monomers on the surfaces of gold nanorods (GNRs). Ciprofloxacin (CIP) is loaded onto polymer shells of nanocomposites with a loading content of 9.8%. The negatively charged nanocomposites switch to positive upon passive accumulation at the infectious sites, which promotes deep biofilm penetration and bacterial adhesion of the nanoparticles. Subsequently, NIR irradiation triggers the nanocomposites to rapidly shrink in volume, further increasing the depth of biofilm penetration. The NIR-triggered, ultrafast volume shrinkage causes an instant release of CIP on the bacterial surface, realizing the synergistic benefits of chemo-photothermal therapy. Both in vitro and in vivo evidence demonstrate that drug-loaded nanocomposites could eradicate clinical MRSA biofilms. Taken together, the multifunctional chemo-photothermal-integrated antimicrobial platform, as designed, is a promising antimicrobial agent against MRSA infections.

show abstract

Section: Introductionmentioning

confidence: 56%

Section: ■ Introductionmentioning

confidence: 70%

Surface-Charge-Switchable and Size-Transformable Thermosensitive Nanocomposites for Chemo-Photothermal Eradication of Bacterial Biofilms in Vitro and in Vivo

Yin

Yang

Yan

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Resistance towards ethidium bromide (Et-Br) is considered a marker of MDR mediated by over expression of efflux pumps [ 6 , 48 , 49 , 50 , 51 , 61 , 62 , 64 , 65 ]. According to the two newest reports, gold nanorods enhance the activity of ciprofloxacin by inhibiting the biofilms and a silver nanocomposite was found as a resistance reversal agent [ 66 , 67 ]. In this study, nanomaterials were found to synergize the partner drugs by inhibiting the biofilm synthesis/transporter proteins.…”

Section: Discussionmentioning

confidence: 99%

Drug Resistance Reversal Potential of Nanoparticles/Nanocomposites via Antibiotic’s Potentiation in Multi Drug Resistant P. aeruginosa

et al. 2021

View full text Add to dashboard Cite

Bacteria employ numerous resistance mechanisms against structurally distinct drugs by the process of multidrug resistance. A study was planned to discover the antibacterial potential of a graphene oxide nanosheet (GO), a graphene oxide–zinc oxide nanocomposite (GO/ZnO), a graphene oxide-chitosan nanocomposite (GO–CS), a zinc oxide decorated graphene oxide–chitosan nanocomposite (GO–CS/ZnO), and zinc oxide nanoparticles (ZnO) alone and in a blend with antibiotics against a PS-2 isolate of Pseudomonas aeruginosa. These nanocomposites reduced the MIC of tetracycline (TET) from 16 folds to 64 folds against a multidrug-resistant clinical isolate. Efflux pumps were interfered, as evident by an ethidium bromide synergy study with nanocomposites, as well as inhibiting biofilm synthesis. These nanoparticles/nanocomposites also decreased the mutant prevention concentration (MPC) of TET. To the best of our knowledge, this is the first report on nanomaterials as a synergistic agent via inhibition of efflux and biofilm synthesis.

show abstract

“…Targeting moieties that are able to find their own way through the body to an infectious biofilm have been suggested based on general properties of an infectious biofilm, most notably its acidity as compared with healthy, surrounding tissue [39]. Photothermal nanoparticles modified with Pep-DA [40], ciprofloxacin with conjugated carboxyl betaine [41], zwitterionic ammonium compounds [42,43] or polyaniline-conjugated glycol chitosan [44,45] become positively-charged in an acidic environment yielding electrostatic double-layer attraction with negatively-charged bacterial cell surface [46]. Often, however, synthesis is laborious [39] and multiple modifications on a single nanoparticle, like achieving stealth and pH-responsiveness, are difficult to balance [47][48][49].…”

Section: Discussionmentioning

confidence: 99%

Encapsulation of Photothermal Nanoparticles in Stealth and pH-Responsive Micelles for Eradication of Infectious Biofilms In Vitro and In Vivo

Gao

et al. 2021

Nanomaterials

View full text Add to dashboard Cite

Photothermal nanoparticles can be used for non-antibiotic-based eradication of infectious biofilms, but this may cause collateral damage to tissue surrounding an infection site. In order to prevent collateral tissue damage, we encapsulated photothermal polydopamine-nanoparticles (PDA-NPs) in mixed shell polymeric micelles, composed of stealth polyethylene glycol (PEG) and pH-sensitive poly(β-amino ester) (PAE). To achieve encapsulation, PDA-NPs were made hydrophobic by electrostatic binding of indocyanine green (ICG). Coupling of ICG enhanced the photothermal conversion efficacy of PDA-NPs from 33% to 47%. Photothermal conversion was not affected by micellar encapsulation. No cytotoxicity or hemolytic effects of PEG-PAE encapsulated PDA-ICG-NPs were observed. PEG-PAE encapsulated PDA-ICG-NPs showed good penetration and accumulation in a Staphylococcus aureus biofilm. Penetration and accumulation were absent when nanoparticles were encapsulated in PEG-micelles without a pH-responsive moiety. PDA-ICG-NPs encapsulated in PEG-PAE-micelles found their way through the blood circulation to a sub-cutaneous infection site after tail-vein injection in mice, yielding faster eradication of infections upon near-infrared (NIR) irradiation than could be achieved after encapsulation in PEG-micelles. Moreover, staphylococcal counts in surrounding tissue were reduced facilitating faster wound healing. Thus, the combined effect of targeting and localized NIR irradiation prevented collateral tissue damage while eradicating an infectious biofilm.

show abstract

Ciprofloxacin conjugated gold nanorods with pH induced surface charge transformable activities to combat drug resistant bacteria and their biofilms

Cited by 26 publications

References 39 publications

Surface-Charge-Switchable and Size-Transformable Thermosensitive Nanocomposites for Chemo-Photothermal Eradication of Bacterial Biofilms in Vitro and in Vivo

Surface-Charge-Switchable and Size-Transformable Thermosensitive Nanocomposites for Chemo-Photothermal Eradication of Bacterial Biofilms in Vitro and in Vivo

Drug Resistance Reversal Potential of Nanoparticles/Nanocomposites via Antibiotic’s Potentiation in Multi Drug Resistant P. aeruginosa

Encapsulation of Photothermal Nanoparticles in Stealth and pH-Responsive Micelles for Eradication of Infectious Biofilms In Vitro and In Vivo

Contact Info

Product

Resources

About