Ciprofloxacin HCl and quercetin functionalized electrospun nanofiber membrane: fabrication and its evaluation in full thickness wound healing

Abstract

Microbial infection and oxidative damage of the fibroblast often results in prolonged and incomplete wound healing. Therefore, there is an increasing demand for a scaffold being effective to prevent any possible infection and neutralize excessively released free radicals. Herein, we designed a PCL-based nanofiber loaded with ciprofloxacin hydrochloride (CHL) and quercetin. Developed nanofiber showed the formation of smooth and continuous nanofiber with 101.59 ± 29.18 nm average diameter and entrapping the drugs in amorphous form without any possible physico-chemical interaction between drugs and excipient. High entrapment efficiency (CHL: 92.04% and Que: 94.32%) and prolonged in-vitro release (for 7 days) demonstrated the capability of scaffold to suppress any probable infection and oxidative damage, which was further confirmed by in-vitro antibacterial and antioxidant activity. The biocompatibility of scaffold for direct application to wound site was evaluated through hemocompatibility and cytocompatibility assay. The wound healing efficacies of nanofiber were assessed using full thickness wound model in rats, which displayed accelerated wound healing with complete reepithelialization and improved collagen deposition within 16 days. In-vivo wound healing finding was further corroborated by SOD, catalase, and hydroxyproline assay. The current study validates the application of ciprofloxacin HCl and quercetin functionalized nanofiber as a potential wound dressing material.

Norfloxacin-Loaded Electrospun Scaffolds: Montmorillonite Nanocomposite vs. Free Drug

“…Pharmaceutics 2020, 12, 325 3 of 24 focused on the enhancement of wound healing using antimicrobial loaded electrospun scaffolds/ dressings [12][13][14][15], those were in large part based on synthetic polymers, as polycaprolactone [12][13][14] or polyethylene glycol [15], and produced using critical solvents such as formic acid [12], or chloroform [15].…”

An Antimicrobial Peptide-Loaded Chitosan/Polyethylene Oxide Nanofibrous Membrane Fabricated by Electrospinning Technology

“…The initial burst release may be due to the immediate contact between the nanofiber membrane and the dissolution medium and the leaching of the drug molecules in close contact with the nanofiber membrane's surface. The longerterm extended-release may be due to the more resonant diffusion of drug molecules in the membrane (Ajmal et al, 2019). It is known to apply sterile dressings to wounds to promote healing and prevent further injury.…”

Regenerative Wound Dressings for Skin Cancer

“…The most suitable hydrogel was obtained at a ratio of gelatin to Carbopol ® of 6 to 4, which exhibited accelerated wound healing and reduced wound closure time albino rat models [ 141 ]. Ajmal et al [ 142 ] designed a wound dressing based on poly(ε-caprolactone) nanofibers loaded with ciprofloxacin hydrochloride and quercetin in order to ensure both anti-bacterial and antioxidant properties. Results showed high entrapment efficiency of more than 92% for both biocompounds and a prolonged in vitro release for 7 days.…”