Circ-ATIC regulates esophageal squamous cell carcinoma growth and metastasis through miR-1294/PBX3 pathway

Zhou, Qian; Lei, Chengang; Cui, Fenghe; Chen, Hao; Cao, Xianzhao

doi:10.1016/j.heliyon.2023.e12916

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…It has been associated with gender, deep invasion, lymph node metastasis and poor prognosis in patients with ESCC ( 54 ). LncSUMO1P3/miR-486-5p/phosducin (PHD) or PHD finger protein 8 regulated CD151 [CD151 molecule (Raph blood group)] expression and promoted the proliferation, migration, invasion, and EMT of KYSE150 and KYSE450 ( 55 ). Prostate cancer associated transcript 5/miR-4295/PHD finger protein 20 upregulated the proliferation, migration and invasion of EC109 and KYSE450 cells ( 56 ).…”

Section: Lncrnas Upregulated In Esccmentioning

confidence: 99%

Roles of long non‑coding RNAs in esophageal cell squamous carcinoma (Review)

Yan,

Wong,

Gong

et al. 2024

Int J Mol Med

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Section: Lncrnas Upregulated In Esccmentioning

confidence: 99%

Roles of long non‑coding RNAs in esophageal cell squamous carcinoma (Review)

Yan,

Wong,

Gong

et al. 2024

Int J Mol Med

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“…Shen et al [ 24 ] constructed a circRNA-miRNA-mRNA interaction network based on ESCC microarray profiles and public databases, and then identified hub genes, validating the significant function of the circRNA-miRNA-mRNA regulatory network in ESCC. Recently, the studies by Wang et al [ 25 ], Zhou et al [ 26 ], and Song et al [ 27 ] enriched the circRNA-miRNA-mRNA regulatory network in ESCC to some extent. Regrettably, a deeper comprehension of the regulatory mechanisms had not been advanced by these studies.…”

Section: Introductionmentioning

confidence: 99%

circRNA6448-14/miR-455-3p/OTUB2 axis stimulates glycolysis and stemness of esophageal squamous cell carcinoma

Zhang,

Wang

et al. 2024

Aging

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Background: Esophageal squamous cell carcinoma (ESCC) is a gastrointestinal malignancy with high incidence. This study aimed to reveal the complete circRNA-miRNA-mRNA regulatory network in ESCC and validate its function mechanism. Method: Expression of OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 2 (OTUB2) in ESCC was analyzed by bioinformatics to find the binding sites between circRNA6448-14 and miR-455-3p, as well as miR-455-3p and OTUB2. The binding relationships were verified by RNA Immunoprecipitation (RIP) and dualluciferase assay. The expressions of circRNA6448-14, miR-455-3p, and OTUB2 were detected by quantitative real-time polymerase chain reaction (qRT-PCR). MTT assay measured cell viability, and the spheroid formation assay assessed the ability of stem cell sphere formation. Western blot (WB) determined the expression of marker proteins of stem cell surface and rate-limiting enzyme of glycolysis. The Seahorse XFe96 extracellular flux analyzer measured the rate of extracellular acidification rate and cellular oxygen consumption. Corresponding assay kits assessed cellular glucose consumption, lactate production, and adenosine triphosphate (ATP) generation. Results: In ESCC, circRNA6448-14 and OTUB2 were highly expressed in contrast to miR-455-3p. Knocking down circRNA6448-14 could prevent the glycolysis and stemness of ESCC cells. Additionally, circRNA6448-14 enhanced the expression of OTUB2 by sponging miR-455-3p. Overexpression of OTUB2 or silencing miR-455-3p reversed the inhibitory effect of knockdown of circRNA6448-14 on ESCC glycolysis and stemness. Conclusion: This research demonstrated that the circRNA6448-14/miR-455-3p/OTUB2 axis induced the glycolysis and stemness of ESCC cells. Our study revealed a novel function of circRNA6448-14, which may serve as a potential therapeutic target for ESCC.

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Roles of non-coding RNAs in the metabolism and pathogenesis of bladder cancer

Sanya

Onésime

2023

Human Cell

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Circ-ATIC regulates esophageal squamous cell carcinoma growth and metastasis through miR-1294/PBX3 pathway

Cited by 7 publications

References 34 publications

Roles of long non‑coding RNAs in esophageal cell squamous carcinoma (Review)

Roles of long non‑coding RNAs in esophageal cell squamous carcinoma (Review)

circRNA6448-14/miR-455-3p/OTUB2 axis stimulates glycolysis and stemness of esophageal squamous cell carcinoma

Roles of non-coding RNAs in the metabolism and pathogenesis of bladder cancer

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