Circ‐MTO1 correlates with favorable prognosis and inhibits cell proliferation, invasion as well as miR‐17‐5p expression in prostate cancer

Hu, Yijia; Guo, Bin

doi:10.1002/jcla.23086

Cited by 22 publications

(41 citation statements)

References 24 publications

(50 reference statements)

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“…Previous prognostic studies on both serum and tissue from PCa patients support our results. However, these studies were all based on RT-PCR 17 , 21 – 23 . Hoey et al .…”

Section: Discussionmentioning

confidence: 99%

High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients

Støen

Andersen

Rakaee

et al. 2021

Sci Rep

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MicroRNAs (miRs) are small non-coding RNA molecules, which are involved in the development of various malignancies, including prostate cancer (PCa). miR-17-5p is considered the most prominent member of the miR-17-92 cluster, with an essential regulatory function of fundamental cellular processes. In many malignancies, up-regulation of miR-17-5p is associated with worse outcome. In PCa, miR-17-5p has been reported to increase cell proliferation and the risk of metastasis. In this study, prostatectomy specimens from 535 patients were collected. Tissue microarrays were constructed and in situ hybridization was performed, followed by scoring of miR-17-5p expression on different tumor compartments. High expression of miR-17-5p in tumor epithelium was associated with biochemical failure (BF, p < 0.001) and clinical failure (CF, p = 0.019). In multivariate analyses, high miR-17-5p expression in tumor epithelial cells was an independent negative prognostic factor for BF (HR 1.87, 95% CI 1.32–2.67, p < 0.001). In vitro analyses confirmed association between overexpression of miR-17-5p and proliferation, migration and invasion in prostate cancer cell lines (PC3 and DU145). In conclusion, our study suggests that a high cancer cell expression of miR-17-5p was an independent negative prognostic factor in PCa.

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“…Previous prognostic studies on both serum and tissue from PCa patients support our results. However, these studies were all based on RT-PCR 17 , 21 – 23 . Hoey et al .…”

Section: Discussionmentioning

confidence: 99%

High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients

Støen

Andersen

Rakaee

et al. 2021

Sci Rep

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“…Overexpression of cir-ITCH inhibited the growth, migration, and invasion of PCa cells, proving its tumor inhibitory function in PCa. Both tumor-promoting as well as tumor-suppressing roles in PCa have been reported for miR-17 [28,32]. Evidence has shown that high or low miR-17 expression in the blood is related to the progression of PCa [33].…”

Section: Discussionmentioning

confidence: 99%

“…The higher expression of circ-MTO1 in tumor tissue compared to normal tissues suggests a better prognosis. Overexpression of circ-MTO1 in prostate cancer cells can inhibit cell viability and the expression of miR-17 [ 28 ]. circRNA circfoxo3 reportedly promotes the development of prostate cancer by sponging miR-29a-3p [ 29 ], while circSMARC5 is upregulated in prostate cancer and its expression is androgen-responsive [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer

Zhang

et al. 2020

BioMed Research International

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cir-ITCH, a well-known tumor-suppressive circular RNA, plays a critical role in different cancers. However, its expression and functional role in prostate cancer (PCa) are unclear. Herein, we explored the potential mechanism and tumor-inhibiting role of cir-ITCH in PCa. Using reverse transcriptase polymerase chain reaction assay, we analyzed the expression of cir-ITCH in PCa and paired adjacent nontumor tissue samples resected during surgical operation, as well as in two cell lines of human PCa (LNCaP and PC-3) and the immortalized normal prostate epithelial cell line (RWPE-1). Cell viability and migration of PCa cell lines were evaluated using CCK-8 and wound-healing assays. Expression of key proteins of the Wnt/β-catenin and PI3K/AKT/mTOR pathways was detected using western blotting. We found that cir-ITCH expression was typically downregulated in the tissues and cell lines of PCa compared to that in the peritumoral tissue and in RWPE-1 cells, respectively. The results showed that cir-ITCH overexpression significantly inhibits the proliferation, migration, and invasion of human PCa cells and that reciprocal inhibition of expression occurred between cir-ITCH and miR-17. Proteins in the Wnt/β-catenin and PI3K/AKT/mTOR pathways were downregulated by overexpression of cir-ITCH both in androgen receptor-positive LNCaP cells and androgen receptor-negative PC-3 cells. Taken together, these data demonstrated that cir-ITCH plays a tumor-suppressive role in human PCa cells, partly through the Wnt/β-catenin and PI3K/AKT/mTOR pathways. Thus, cir-ITCH may serve as a novel therapeutic target for the treatment of PCa, especially castration-resistant prostate cancer.

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“…The circRNAs identified in this study have not been reported previously. Therefore, we performed a literature review of circRNAs in PCa in the PubMed database, identifying 13 studies ( Xia et al, 2018 ; Shen et al, 2019 ; Song et al, 2019 ; Wang et al, 2019 ; Wu et al, 2019 ; Hu and Guo, 2020 ; Kong et al, 2020 ; Li T. et al, 2020 ; Ou et al, 2020 ; Shan et al, 2020 ; Yan et al, 2020 ; Zheng et al, 2020a , b ) of 18 circRNA–miRNA interactions. The miRNAs identified in this study have previously been shown to play important roles in PCa.…”

Section: Discussionmentioning

confidence: 99%

Identification of Prostate Cancer-Related Circular RNA Through Bioinformatics Analysis

Lin

Chen

et al. 2020

Front. Genet.

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Background: Prostate cancer (PCa) is one of the most common malignant tumors worldwide. Accumulating evidence has suggested that circular RNAs (circRNAs) are involved in the development and progression of various cancers, and they show great potential as novel biomarkers. However, the underlying mechanisms and specific functions of most circRNAs in PCa remain unknown. Here, we aimed to identify circRNAs with potential roles in PCa from the PCa expression profile. Methods: We used data downloaded from the Gene Expression Omnibus to identify circRNAs that were differentially expressed between PCa samples and adjacent nontumor samples. Relative expression levels of identified circRNAs were validated by quantitative real-time PCR. Micro (mi)RNA response elements were predicted by the CircInteractome database, and miRNA target genes were predicted by miRDB, miRTarBase, and TargetScan databases. Gene ontology (GO) enrichment analysis and pathway analysis revealed the potential biological and functional roles of these target genes. A circRNA-miRNA-mRNA interaction network was constructed by Cytoscape. The interaction between circRNAs and miRNAs in PCa was thoroughly reviewed in the PubMed. Finally, the mRNA expression of these genes was validated by the Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA) databases. The expression of proteins encoded by these genes was further validated by the Human protein Atlas (HPA) database. Results: A total of 60 circRNAs that were differentially expressed between PCa and healthy samples were screened, of which 15 were annotated. Three circRNAs (hsa_circ_0024353, hsa_circ_0085494, hsa_circ_0031408) certified the criteria were studied. The results of quantitative real-time PCR demonstrated that the expression of hsa_circ_0024353 was significantly downregulated in PC-3 cells when compared with RWPE-1 cells, while the expression of hsa_circ_0031408 and hsa_circ_0085494 was significantly upregulated in PC-3 cells when compared with RWPE-1 cells. GO and Kyoto Encyclopedia of Genes and Genomes analyses found that target genes were mainly enriched in metabolic processes and pathways involving phosphoinositide 3-kinase-Akt signaling, hypoxia-inducible factor-1 signaling, p53 signaling, and the cell cycle. A total of 11 miRNA target genes showing differential expression between

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Circ‐MTO1 correlates with favorable prognosis and inhibits cell proliferation, invasion as well as miR‐17‐5p expression in prostate cancer

Cited by 22 publications

References 24 publications

High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients

High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients

Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer

Identification of Prostate Cancer-Related Circular RNA Through Bioinformatics Analysis

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