Circ‐OPHN1 suppresses the proliferation, migration, and invasion of trophoblast cells through mediating miR‐558/THBS2 axis

Li, Yu; Chen, Jinzao; Song, Shuqin

doi:10.1002/ddr.21931

Cited by 9 publications

(2 citation statements)

References 44 publications

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“…Furthermore, THBS2 is a metastatic‐related gene in different cancers 24,36 . High expression of THBS2 was measured in PE samples, and circ‐AK2, 37 hsa_circ_0085296, 38 and circ‐OPHN1 39 could repress trophoblast proliferation, invasion, and migration through upregulating THBS2. In this research, THBS2 could be targeted by miR‐616‐3p.…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression

Wang,

Liu,

Wang

et al. 2023

American J Rep Immunol

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BackgroundPreeclampsia (PE) is a hypertensive disorder of pregnancy that causes significant maternal and perinatal morbidity and mortality. Circular RNA (circRNA) hsa_circ_0015382 is associated with the pathogenesis of PE, but its underlying regulatory mechanism remains to be explored.MethodsRelative RNA levels of hsa_circ_0015382, microRNA‐616‐3p and thrombospondin‐2 (THBS2) were detected by quantitative reverse transcription‐polymerase chain reaction. In vitro regulatory effects of hsa_circ_0015382 on the proliferation, migration, invasion and angiogenesis of trophoblasts were evaluated by CCK‐8, flow cytometry for cell cycle, EdU, transwell, wound healing and HUVEC tube formation assays, respectively. Targeting interaction was verified by dual‐luciferase reporter and RNA immunoprecipitation assays.ResultsHsa_circ_0015382 was highly expressed in placental tissues from PE patients. Upregulation of hsa_circ_0015382 repressed trophoblast proliferation, migration, invasion and lowered trophoblast‐induced HUVEC tube formation. Hsa_circ_0015382 was validated as a miR‐616‐3p sponge and miR‐616‐3p targeted THBS2. Hsa_circ_0015382 could mediate trophoblast proliferation, migration, invasion and regulate trophoblast‐induced HUVEC tube formation by sponging miR‐616‐3p and regulating THBS2 expression.ConclusionHsa_circ_0015382 is associated with the pathogenesis of PPE by regulating the miR‐616‐3p/THBS2 axis.Highlights Hsa_circ_0015382 is overexpressed in preeclampsia patients. Hsa_circ_0015382 inhibits trophoblast proliferation, migration, invasion and decreases trophoblast‐induced HUVEC tube formation. Hsa_circ_0015382 interacts with miR‐616‐3p to regulate THBS2 expression.

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Section: Discussionmentioning

confidence: 99%

Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression

Wang,

Liu,

Wang

et al. 2023

American J Rep Immunol

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“…The cytotoxicity was tested on seven cell lines (SW480, HL-60, HepG-2, A375, MCF-7, CaoV-3, and A549) by MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] assay as previously described. , …”

Section: Methodsmentioning

confidence: 99%

Gene-Directed Generation of Unprecedented Bioactive Compounds

Lin

Shi

et al. 2023

ACS Sustainable Chem. Eng.

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Bioactive compounds with previously undescribed frameworks are highly desired for the discovery and development of new drugs and agrochemicals, but very few attempts have been reported to generate such molecules in biological contexts. Here, we present a gene-directed generation of architecturally unprecedented polyketide–indole hybrids (PIHs), which was conceptualized and materialized by employing polyketide synthases expressed in a heterologous vector, with simultaneous exposure to exogenous chemicals. To make an exemplification to this generally applicable approach, the ChrA and ChrB genes of Daldinia eschscholzii IFB-TL01 were integrated into the Aspergillus oryzae (AO) cell, and the resultant ChrA/ChrB-AO transformant was cultured in the indole-3-carbinol (I3C)-supplemented medium, leading to the production of seven skeletally undescribed PIHs named aochrabines A–G. Among them, aochrabines A–C exhibited a broad spectrum in inhibiting the growth of Gram-positive bacteria, whereas aochrabines B, C, and G showed moderate antitumor activities. Unexpectedly, the construction of such aochrabine molecules was achieved by the regioselective Michael addition of 3-methyleneindolium (3MI, generated from I3C in the AO culture) to different polyketide precursors with the yields (much) higher than those in the D. eschscholzii culture where comparable. Chemically, the benzyl-methine carbons in the precursor molecules were found to be made more vulnerable to the 3MI attack by the hydrogen-bonding between the ortho-hydroxyl and meta-carbonyl groups. Collectively, this is the first report of the ortho- and meta-substituent co-driven regioselective Michael addition of electrophilic methylene compounds to heterologous PKS production platform to in situ multiply the chemodiversity of microbial cultures, thus showing great potential in producing valuable compounds with new chemical space.

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GATSDCD: Prediction of circRNA-Disease Associations Based on Singular Value Decomposition and Graph Attention Network

Niu

Hesham

Zou

2022

Intelligent Computing Theories and Application

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Circ‐OPHN1 suppresses the proliferation, migration, and invasion of trophoblast cells through mediating miR‐558/THBS2 axis

Cited by 9 publications

References 44 publications

Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression

Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression

Gene-Directed Generation of Unprecedented Bioactive Compounds

GATSDCD: Prediction of circRNA-Disease Associations Based on Singular Value Decomposition and Graph Attention Network

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