Circ‐UBAP2 functions as sponges of miR‐1205 and miR‐382 to promote glioma progression by modulating STC1 expression

Wang, Jianxin; Li, Tianxiao; Wang, Bin

doi:10.1002/cam4.3759

Cited by 27 publications

(10 citation statements)

References 30 publications

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“…Numerous studies have shown that circRNAs in the cytoplasm primarily exert their roles by serving as “miRNA sponges,” which regulate the expression of mRNAs by targeting their 3′UTR region 29,30 . Numerous investigations have demonstrated that “miRNA sponge” is how cytoplasmic circRNAs carry out their biological role 17,31 . And it was discovered that the majority distribution of circFAM114A2 was present in the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

CircFAM114A2 inhibits the progression of hepatocellular carcinoma via miR‐630/HHIP axis

Lai

Liu

et al. 2023

Cancer Medicine

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Background Many studies have shown that circular RNAs (circRNAs) are abnormally expressed in various tumor tissues and served as a key regulator in the occurrence and development of cancer. However, in hepatocellular carcinoma (HCC), the molecular mechanism of circRNAs in body fluids remains to be further explored. Methods The expression levels of genes and proteins were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting, respectively. Cell counting Kit‐8 (CCK‐8), 5‐Ethynyl‐2'‐deoxyuridine (EdU), wound healing assay, Transwell assays, flow cytometry, and tumor formation models in nude mice were conducted to investigate the effects of circFAM114A2 on HCC cells both in vitro and in vivo. RNA antisense purification (RAP), dual luciferase reporter assays and rescue assays were carried out to verify the interaction between circFAM114A2, miR‐630 and HHIP. Results CircFAM114A2 was significantly downregulated in HCC tissues and was associated with microvascular invasion and lymph node metastasis of HCC patients. We also observed that circFAM114A2 was lowly expressed in HCC plasma, which may serve as an effective biomarker to screen HCC patients from healthy controls (area under curve (AUC)=0.922). In vitro, circFAM114A2 overexpression significantly blunted HCC cell proliferation, migration, invasion, and promoted apoptosis, whereas circFAM114A2 silencing posed opposite effects. In vivo, circFAM114A2 overexpression inhibited the growth of HCC cells. Mechanistically, circFAM114A2 could increase the expression of the tumor suppressor HHIP via acting as a sponge for miR‐630. Conclusions CircFAM114A2 exerts a tumor suppressor role in HCC through miR‐630/HHIP axis, and may be served as a potential diagnostic and therapeutic biomarker for HCC patients.

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Section: Discussionmentioning

confidence: 99%

CircFAM114A2 inhibits the progression of hepatocellular carcinoma via miR‐630/HHIP axis

Lai

Liu

et al. 2023

Cancer Medicine

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“…Additionally, a previous study revealed that expression of the miR-382 family is signi cantly higher in glioma patients, compared with healthy person, and is crucial in cancer formation and progression 21 . Moreover, it has been reported that a reduction in mature miR-382 is associated with the proliferation and invasion of glioblastoma cells 22 . However, the miR-382-associated signaling network,…”

Section: Discussionmentioning

confidence: 99%

Bone marrow mesenchymal stem cell-derived exosomal microRNA-382 promotes osteogenesis in osteoblast via regulation of SLIT2

Su¹,

Yang²,

Lv³

et al. 2022

Preprint

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Background: Osteoporosis (OP) is a systemic skeletal disorder with increased bone fragility. Human bone marrow mesenchymal stem cells (hBMSCs), have multi-lineage differentiation ability, which may play important roles in osteoporosis. In this study, we aim to investigate the role of hBMSCs-derived miR-382 in osteogenic differentiation. Methods: The miRNA and mRNA expression in peripheral blood monocytes between persons with high or low bone mineral density (BMD) were compared. Then we collected the hBMSCs-secreted sEV and examined the dominant components. The over-expression of the miR-382in MG63 cell and its progression of osteogenic differentiation were investigated by qRT-PCR, western blot and alizarin red staining. The interaction between miR-382and SLIT2 was confirmed by dual luciferase assay. The role of SLIT2 was also confirmed through up-regulation in MG63 cell, and the osteogenic differentiation-associated gene and protein were tested. Results: According to bioinformatic analysis, a series of differential expressed genes between persons with high or low BMD were compared. After internalization of hBMSC-sEV in MG63 cells, we observed that the ability of osteogenic differentiation was significantly enhanced. Similarly, after up-regulation of miR-382 in MG63 cells, osteogenic differentiation was also promoted. According to the dual luciferase assay, the targeting function of miR-382 in SLIT2 was demonstrated. Moreover, the benefits of hBMSC-sEV in osteogenesis was abrogated through up-regulation of SLIT2. Conclusion: Our study provided evidence that miR-382-contained hBMSC-sEV held great promise in osteogenic differentiation in MG63 cells after internalization by targeting SLIT2, which can be served as molecular targets to develop effective therapy.

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“…As previously described [ 19 ], LN229 and U251 cells were harvested and then plated into 6-well plates (150 cells). The cells were cultured at 37°C for 14 days.…”

Section: Methodsmentioning

confidence: 99%

circ_0030018 promotes glioma proliferation and metastasis

Shao

Yang

Miao

et al. 2021

Translational Neuroscience

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Background Circular RNA (circRNA) plays an essential role in tumor progression, including glioma. circ_0030018 is a newly discovered circRNA that is highly expressed in glioma. However, its role and mechanism in glioma need to be further elucidated. Methods The expression of circ_0030018, microRNA (miR)-194-5p, and tripartite motif containing 44 (TRIM44) was examined using quantitative real-time PCR. Cell proliferation, migration, invasion, and apoptosis were determined using MTT assay, colony formation assay, transwell assay, and flow cytometry. Moreover, dual-luciferase reporter assay and RNA pull-down assay were used to verify the interactions among circ_0030018, miR-194-5p, and TRIM44. The protein expression of TRIM44 was assessed by western blot analysis. Animal experiments were conducted to explore the role of circ_0030018 in glioma tumor growth in vivo. Results circ_0030018 was overexpressed in glioma tissues and cells, and its silencing could inhibit glioma cell proliferation, migration, invasion, and accelerate apoptosis. miR-194-5p could be sponged by circ_0030018, and its overexpression could hinder the progression of glioma cells. Further experiments revealed that miR-194-5p inhibitor reversed the negative regulation of circ_0030018 knockdown on glioma cell progression. In addition, TRIM44 was a target of miR-194-5p, and its downregulation could repress glioma cell progression. Overexpressed TRIM44 reversed the inhibition effect of miR-194-5p on glioma cell progression. Animal experiments suggested that circ_0030018 knockdown could reduce glioma tumor growth through regulating miR-194-5p and TRIM44. Conclusion Our 8data showed that circ_0030018 enhanced glioma progression by sponging miR-194-5p to regulate TRIM44, indicating that circ_0030018 might be a potential treatment target for glioma.

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Circ‐UBAP2 functions as sponges of miR‐1205 and miR‐382 to promote glioma progression by modulating STC1 expression

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 27 publications

References 30 publications

CircFAM114A2 inhibits the progression of hepatocellular carcinoma via miR‐630/HHIP axis

CircFAM114A2 inhibits the progression of hepatocellular carcinoma via miR‐630/HHIP axis

Bone marrow mesenchymal stem cell-derived exosomal microRNA-382 promotes osteogenesis in osteoblast via regulation of SLIT2

circ_0030018 promotes glioma proliferation and metastasis

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