circ0093740 Promotes Tumor Growth and Metastasis by Sponging miR-136/145 and Upregulating DNMT3A in Wilms Tumor

Cao, Juan; Huang, Zi Lin; Ou, Shunling; Wen, Feiqiu; Yang, Guocheng; Miao, Qiuling; Huang, Zhuowei; Wang, Yue; He, Xiaomin; Shan, Yingying; Liu, Sixi; Jiang, Lijuan

doi:10.3389/fonc.2021.647352

Cited by 12 publications

(13 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following a previous analysis of high-throughput microarray sequencing data by our group ( 26 ), RT-qPCR was performed in the present study to verify the expression level of the top five downregulated circRNAs in 10 pairs of kidney cancer samples and adjacent normal kidney samples ( Fig. 1A ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

circKL inhibits the growth and metastasis of kidney cancer by sponging miR‑182‑5p and upregulating FBXW7

Cao

et al. 2022

Oncol Rep

Self Cite

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are a type of non-coding RNA with important roles in the regulation of various biological processes involved in malignant progression. However, the potential molecular mechanisms and roles of circRNAs in kidney cancer have remained to be fully elucidated. In a previous study by our group, high-throughput microarray sequencing data were analyzed to determine the differentially expressed circRNAs in kidney cancer. In this analysis, a novel circRNA (hsa_circ_0100312, named circKL) was identified as a frequently downregulated circRNA in kidney cancer cells and tissues by reverse transcription-quantitative PCR. In the present study, Cell Counting Kit-8, colony formation, Transwell, wound-healing and mouse xenograft assays as well as a lung metastasis experiment were performed to confirm the functions of circKL. The experiments confirmed that circKL overexpression significantly inhibited the proliferation, migration, tumor growth and metastasis of kidney cancer both in vitro and in vivo. The potential molecular mechanisms of circKL were investigated by performing dual-luciferase and RNA immunoprecipitation assays. Western blot assays confirmed that overexpression of circKL significantly increased the protein level of F-box and WD repeat domain containing 7 (FBXW7). All results suggested that circKL suppressed the growth and migration of kidney cancer by sponging microRNA (miR)-182-5p and upregulating FBXW7 expression. Overall, the circKL/miR-182-5p/FBXW7 axis was indicated to have a key role in the growth and metastasis of kidney cancer and may be targeted as a novel therapeutic strategy.

show abstract

Section: Resultsmentioning

confidence: 99%

“…For the present study, high-throughput circRNA microarray data from a previous study by our group were analyzed to screen for the differentially expressed circRNAs in three pairs of kidney cancer tissues ( 26 ). circKL was identified as a significantly downregulated circRNA in both kidney cancer cells and tissues.…”

Section: Discussionmentioning

confidence: 99%

circKL inhibits the growth and metastasis of kidney cancer by sponging miR‑182‑5p and upregulating FBXW7

Cao

et al. 2022

Oncol Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…CLASP2 has been linked to the EMT and early progression of bladder cancer [ 54 ] and has also been shown to predict bladder cancer prognosis [ 55 ]. In several malignancies, DNMT3A has been shown to influence cellular apoptosis [ 56 ], cisplatin resistance [ 57 ], tumor growth, and metastasis [ 58 ]. NCK1-AS1 enhances the expression of NCK1 to aid carcinogenesis and chemo-resistance in ovarian cancer, according to Chang et al [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

The pattern of alternative splicing in lung adenocarcinoma shows novel events correlated with tumorigenesis and immune microenvironment

Wang

Shen

et al. 2021

BMC Pulm Med

View full text Add to dashboard Cite

Lung adenocarcinoma (LUAD) is the leading cause of cancer deaths worldwide due to the lack of early diagnostic markers and specific drugs. Previous studies have shown the association of LUAD growth with aberrant alternative splicing (AS). Herein, clinical data of 535 tumor tissues and 59 normal tissues were extracted from The Cancer Genome Atlas (TCGA) database. Each sample was analyzed using the ESTIMATE algorithm; a comparison between higher and lower score groups (stromal or immune) was made to determine the overall- and progression-free survival-related differentially expressed AS (DEAS) events. We then performed unsupervised clustering of these DEASs, followed by determining their relationship with survival rate, immune cells, and the tumor microenvironment (TME). Next, two prognostic signatures were developed using bioinformatics tools to explore the prognosis of cases with LUAD. Five OS- and six PFS-associated DEAS events were implemented to establish a prognostic risk score model. When compared to the high-risk group (HRG), the PFS and OS of the low-risk group (LRG) were found to be considerable. Additionally, a better prognosis was found considerably associated with the ESTIMATE score of the patients as well as immune cells infiltration. Our analysis of AS events in LUAD not only helps to clarify the tumorigenesis mechanism of AS but also provides ideas for revealing potential prognostic biomarkers and therapeutic targets.

show abstract

“…Cao et al found that hsa_circ_0093740 expression was significantly increased in WT and inhibition of hsa_circ_0093740 significantly suppressed the proliferation and migration of WT by high-throughput microarray sequencing. Mechanistic studies revealed that the hsa_circ_0093740-miR-136/145-DNMT3A axis plays an important regulatory role in WT growth and metastasis ( Cao et al, 2021 ).…”

Section: Roles and Significance Of Circrnas In Pediatric Malignant Solid Tumorsmentioning

confidence: 99%

Emerging Role and Mechanism of circRNAs in Pediatric Malignant Solid Tumors

Shen

Liu

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are non-coding RNAs with covalent closed-loop structures and are widely distributed in eukaryotes, conserved and stable as well as tissue-specific. Malignant solid tumors pose a serious health risk to children and are one of the leading causes of pediatric mortality. Studies have shown that circRNAs play an important regulatory role in the development of childhood malignant solid tumors, hence are potential biomarkers and therapeutic targets for tumors. This paper reviews the biological characteristics and functions of circRNAs as well as the research progress related to childhood malignant solid tumors.

show abstract

circ0093740 Promotes Tumor Growth and Metastasis by Sponging miR-136/145 and Upregulating DNMT3A in Wilms Tumor

Cited by 12 publications

References 38 publications

circKL inhibits the growth and metastasis of kidney cancer by sponging miR‑182‑5p and upregulating FBXW7

circKL inhibits the growth and metastasis of kidney cancer by sponging miR‑182‑5p and upregulating FBXW7

The pattern of alternative splicing in lung adenocarcinoma shows novel events correlated with tumorigenesis and immune microenvironment

Emerging Role and Mechanism of circRNAs in Pediatric Malignant Solid Tumors

Contact Info

Product

Resources

About