Circ_0003570 Suppresses the progression of hepatocellular carcinoma through miR-182-5p/STARD13 regulatory axis

Abstract

Background Emerging evidence have revealed that circRNAs exert important biological effects in the development and progression of various diseases, including cancer. Our study aimed to elaborated the biological effects of hsa-circ_0003570 in hepatocellular carcinoma (HCC) development at the molecular level. Results The results of functional experiments showed that knockdown of circ_0003570 induced HCC cell growth, migration and invasion, whereas overexpression of circ_0003570 presented the opposite effects. In vivo experiments, xenograft tumors grown from circ-overexpressed cells had smaller tumor volume and weight than the control group. Further investigations suggested that circ_0003570 may function as a competing endogenous RNA via competitively binding miR-182-5p and thereby regulating the repression of downstream target gene STARD13, which were demonstrated by dual luciferase reporter assay and functional rescued experiments. Conclusions Taken together, circ_0003570 suppresses the development of HCC by modulating miR-182-5p/STARD13 axis.

Tissue Circular RNA_0004018 and 0003570 as Novel Prognostic Biomarkers for Hepatitis B-Related Hepatocellular Carcinoma

“…In a previous study, the high expression of miR-182-5p activated the Wnt/β-catenin signaling pathway by interfacing with β-catenin degradation, leading to unfavorable prognoses, including early recurrence in HCC patients that underwent surgery [25]. Recently, Zhang et al demonstrated that the overexpression of circ_0003570 suppressed HCC progression by regulating the miR-182-5p/STARD 13 axis [26]. Similar to the tumor suppressor role of hsa_circ_0004018 by inhibiting the Wnt/β-catenin signaling pathway, hsa_circ_0003570 may function as an HCC tumor suppressor by sponging miR-182-5p, leading to the inactivation of Wnt/β-catenin signaling pathway.…”