Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

Abstract

Circular RNAs (circRNAs) are associated with chemoresistance in many cancers. However, the function of circ_0005198 in the temozolomide (TMZ) resistance of glioma has not been well elucidated. Here, we demonstrated that circ_0005198 was considerably up-regulated in glioma tissues, serum samples and TMZ-resistant glioma cells. Silencing of circ_0005198 restrained TMZ resistance, restricted the proliferation and facilitated the apoptosis of TMZ-resistant glioma cells. MiR-198 could be sponged by circ_0005198, and we demonstrated that the effect of circ_0005198 on the progression of TMZ-resistant glioma cells was attributed to the inhibition of miR-198 activity. Moreover, TRIM14 was a target of miR-198 and silencing of TRIM14 hindered TMZ resistance and suppressed the progression of TMZ-resistant glioma cells, while TRIM14 over-expression rescued the inhibiting effect of miR-198 over-expression. We conclude that circ_0005198-miR-198-TRIM14 regulatory pathway is critical to TMZ resistance of glioma.

Bioinformatics Analysis of Stem Cell circ-ASB3 Signaling Pathway and Its Affection on Glioma Biological Characteristics

“…Most abnormally expressed miRNAs appeared to act as oncogenes and were implicated in the malignant transformation of glioma. The EGFR, PI3K/Akt, p53, TGF-dependent apoptotic signaling cascades, Notch and NF-κB pathways were among the most important pathways, while miRNAs played a key role in regulating these pathways, indicating a close relationship with distinct malignant glioma morphologies (Novakova et al, 2009 ; Zhang et al, 2009 ; Zhou et al, 2010 ; Li et al, 2015 ; Cao et al, 2019 ; Deng et al, 2020 ).…”

The Role of Circular RNAs in the Drug Resistance of Cancers

“…In glioma, exosomal circ_0072083 modulates NANOG and ALKBH5 by targeting miR-1252-5p and demethylation to control TMZ resistance ( 114 ). Hsa_circ_0110757 and circ_0005198 facilitate TMZ resistance and inhibit glioma cell apoptosis through the miR-1298-5p/ITGA1 and miR-198/TRIM14 axis, respectively ( 115 , 116 ). Consistent with these findings, downregulation of circ-VPS18, hsa_circ_0000936, circHIPK3, and circ CEP128 improve TMZ sensitivity and repress glioma progression ( 117 – 120 ).…”

NcRNAs: Multi‑angle participation in the regulation of glioma chemotherapy resistance (Review)

“…It competitively binds to miR-198 to regulate the expression of tripartite motif-containing 14. Knocking down circ_0005198 expression directly limits the biological behaviours of drug-resistant gliomas and significantly increases tumour sensitivity to TMZ ( 153 ). In addition, circRNAs target ABC transporters, generating drug resistance through the promotion of drug excretion.…”