Circ_0005927 Inhibits the Progression of Colorectal Cancer by Regulating miR-942-5p/BATF2 Axis

Abstract

Background Colorectal cancer (CRC) is one of the most common aggressive neoplasms worldwide. Circular RNAs (circRNAs) have been involved in the biological process of CRC. This study aimed to explore the effects of circ_0005927 on CRC progression and underneath mechanism. Materials and Methods The expression of circ_0005927, microRNA-942-5p (miR-942-5p) and basic leucine zipper ATF-like transcription factor 2 (BATF2) was detected by quantitative real time polymerase chain reaction (qRT-PCR). The protein expression of BATF2 was determined by Western blot. The effects among circ_0005927, miR-942-5p and BATF2 on cell colony-forming ability, apoptosis and migratory and invasive abilities were revealed by cell colony formation, flow apoptosis and transwell assays, respectively. The associated relationship between miR-942-5p and circ_0005927 or BATF2 was predicted by Circinteractome or TargetScan online database, and identified by dual-luciferase reporter or RNA immunoprecipitation (RIP) assay. The impacts of circ_0005927 overexpression on tumor growth in vivo were investigated by in vivo tumor formation assay. Results Circ_0005927 expression and the mRNA and protein expression of BATF2 were dramatically downregulated, while miR-942-5p expression was obviously upregulated in CRC tissues or cells compared with control groups. Circ_0005927 overexpression repressed cell colony-forming ability, migration and invasion, whereas induced cell apoptosis in CRC; however, these impacts were hindered by miR-942-5p mimic or BATF2 knockdown. Furthermore, circ_0005927 was a sponge of miR-942-5p, and miR-942-5p bound to BATF2. In addition, circ_0005927 overexpression repressed tumor growth in vivo. Conclusion Circ_0005927 suppressed cell colony-forming ability, migration and invasion, and promoted cell apoptosis by sponging miR-942-5p to induce BATF2 in CRC. The possible mechanism provides a theoretical basis for the study of circRNA-directed therapy for CRC.

A Novel Circular RNA CircRFX3 Serves as a Sponge for MicroRNA-587 in Promoting Glioblastoma Progression via Regulating PDIA3

“…circ_0002162 promotes the malignant development of tongue cancer ( Zhang et al, 2021a ). Additionally, the overexpression of circ_0005927 restrains the development of colorectal carcinoma ( Yu et al, 2021b ). Moreover, circ_0015326 facilitates the invasion, migration, and proliferation of ovarian cancer ( Zhang et al, 2021b ).…”

Construction of the circRNA-miRNA-mRNA Regulatory Network of an Abdominal Aortic Aneurysm to Explore Its Potential Pathogenesis

“…It has been initially indicated that circRNAs could mediate miRNA functions (e.g., via sponging) and control important events in transcription (e.g., RNA folding and endonuclease protection) [19]. In addition, it has been demonstrated in current evidence that circRNAs contain multiple microRNA response elements (MREs) that can bind to miRNAs, commonly called "miRNA sponges," which could relieve the targeted inhibition of downstream mRNAs by miRNAs [29][30][31]. In this study, the underlying molecular mechanisms of AAAs have been investigated based on ceRNA regulatory hypothesis of circRNAs, miRNA, and mRNA.…”

Nuclear export of BATF2 enhances colorectal cancer proliferation through binding to CRM1

“… 16 Therefore, subsequent studies, including ours, mainly focused on gene expression regulation through exploring the upstream transcriptional factors, microRNAs, circular RNAs and N6‐methyladenosine modification of BATF2. 12 , 14 , 15 , 17 , 18 , 19 , 20 Our previous work also identified a transcriptional factor, glucocorticoid receptor, which could induce BATF2 expression in lymphoma cells. 12 However, these studies have still not clarified the cause of the reduced tumour suppressive effect of BATF2.…”