Circ_0007534 Silencing Inhibits the Proliferation, Migration and Invasion and Induces the Apoptosis of Glioma Cells Partly Through Down-Regulating PROX1 Via Elevating miR-22-3p Level

Yong, Zheng; Wang, Yan; Mai, Rongkang; Liu, Liang; Zhu, Zhihong; Cao, Yiyao

doi:10.1007/s10571-021-01150-y

Cited by 6 publications

(2 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With regards to the PDHGG-WT cell line, we found miR-203a and miR-877-5p in the exosomes isolated from the clone 5E2, while miR-572, miR-376a-3p, and miR-22-3p were exclusively identified in the exosomes isolated from the 1D3 clone. It has been demonstrated that miR-877-5p, miR-376a-3p and miR-22-3p are involved in the regulation of glioma cell proliferation, dissemination, and resistance to treatments [ 79 – 81 ]. These results demonstrate that the single-cell-derived clones co-existing within the same tumour have different exosomal miRNA cargos providing novel evidence of the intra-tumour heterogeneity in PDHGG.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas

Pericoli,

Galardi,

Paolini

et al. 2023

Cell Biosci

View full text Add to dashboard Cite

Background Paediatric-type diffuse High-Grade Gliomas (PDHGG) are highly heterogeneous tumours which include distinct cell sub-populations co-existing within the same tumour mass. We have previously shown that primary patient-derived and optical barcoded single-cell-derived clones function as interconnected networks. Here, we investigated the role of exosomes as a route for inter-clonal communication mediating PDHGG migration and invasion. Results A comprehensive characterisation of seven optical barcoded single-cell-derived clones obtained from two patient-derived cell lines was performed. These analyses highlighted extensive intra-tumour heterogeneity in terms of genetic and transcriptional profiles between clones as well as marked phenotypic differences including distinctive motility patterns. Live single-cell tracking analysis of 3D migration and invasion assays showed that the single-cell-derived clones display a higher speed and longer travelled distance when in co-culture compared to mono-culture conditions. To determine the role of exosomes in PDHGG inter-clonal cross-talks, we isolated exosomes released by different clones and characterised them in terms of marker expression, size and concentration. We demonstrated that exosomes are actively internalized by the cells and that the inhibition of their biogenesis, using the phospholipase inhibitor GW4689, significantly reduced the cell motility in mono-culture and more prominently when the cells from the clones were in co-culture. Analysis of the exosomal miRNAs, performed with a miRNome PCR panel, identified clone-specific miRNAs and a set of miRNA target genes involved in the regulation of cell motility/invasion/migration. These genes were found differentially expressed in co-culture versus mono-culture conditions and their expression levels were significantly modulated upon inhibition of exosome biogenesis. Conclusions In conclusion, our study highlights for the first time a key role for exosomes in the inter-clonal communication in PDHGG and suggests that interfering with the exosome biogenesis pathway may be a valuable strategy to inhibit cell motility and dissemination for these specific diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas

Pericoli,

Galardi,

Paolini

et al. 2023

Cell Biosci

View full text Add to dashboard Cite

show abstract

“…Interestingly, upon collecting and studying 45 glioma tissues from glioma patients and 23 normal brain tissues from normal controls for clinical experiments, Zheng et al. ( 84 ) also found that circ_0007534 was significantly overexpressed in cancer tissues and verified that silencing circ_0007534 inhibited cell migration and invasion. In contrast, Zheng et al.…”

Section: Circ_0007534 Dysregulation In Cancers and Its Mechanismsmentioning

confidence: 99%

Circ_0007534 as new emerging target in cancer: Biological functions and molecular interactions

Liu

Dong²,

Chen³

et al. 2022

Front. Oncol.

View full text Add to dashboard Cite

Circular RNA (circRNAs), an important member of the non-coding RNA (ncRNA) family, are widely expressed in a variety of biological cells. Owing to their stable structures, sequence conservations, and cell- or tissue-specific expressions, these RNA have become a popular subject of scientific research. With the development of sequencing methods, it has been revealed that circRNAs exert their biological function by sponging microRNAs (miRNAs), regulating transcription, or binding to proteins. Humans have historically been significantly impacted by various types of cancer. Studies have shown that circRNAs are abnormally expressed in various cancers and are involved in the occurrence and development of malignant tumors, such as tumor cell proliferation, migration, and invasion. As one of its star molecules, circ_0007534 is upregulated in colorectal, cervical, and pancreatic cancers; is closely related to the occurrence, development, and prognosis of tumors; and is expected to become a novel tumor marker and therapeutic target. This article briefly reviews the expression and mechanism of circ_0007534 in malignant tumors based on the domestic and foreign literature.

show abstract

Circ_0007534 promotes cholangiocarcinoma stemness and resistance to anoikis through DDX3X-mediated positive feedback regulation of parental gene DDX42

Liu,

Guan,

Sha

et al. 2024

Cellular Signalling

View full text Add to dashboard Cite

Circ_0007534 Silencing Inhibits the Proliferation, Migration and Invasion and Induces the Apoptosis of Glioma Cells Partly Through Down-Regulating PROX1 Via Elevating miR-22-3p Level

Cited by 6 publications

References 46 publications

Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas

Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas

Circ_0007534 as new emerging target in cancer: Biological functions and molecular interactions

Circ_0007534 promotes cholangiocarcinoma stemness and resistance to anoikis through DDX3X-mediated positive feedback regulation of parental gene DDX42

Contact Info

Product

Resources

About