circ_0038718 promotes colon cancer cell malignant progression via the miR-195-5p/Axin2 signaling axis and also effect Wnt/β-catenin signal pathway

Abstract

Objective Colon cancer (CC) is one of the most common cancers whose progression is regulated by a number of factors, including circular RNAs (circRNAs). Nonetheless, circ_0038718 is a novel circRNA, and its regulatory mechanism in CC remains unclear. Methods Real-time quantitative PCR (qRT-PCR) was performed to detect the expression of circ_0038718, miR-195-5p and Axin2. Western blot was conducted to determine the protein expression of Axin2 and the key proteins on Wnt/β-catenin signaling pathway. Oligo (dT) 18 primers and RNase R were employed to identify the circular features of circ_0038718, and the location of circ_0038718 in cells was detected via nucleocytoplasmic separation. Dual-luciferase reporter assay and RNA binding protein immunoprecipitation experiment were carried out to investigate the molecular mechanism of circ_0038718/miR-195-5p/Axin2. Additionally, MTT assay was conducted to assess cell proliferation; Transwell assay was performed to evaluate cell migration and invasion, respectively. The effect of circ_0038718 on CC tumor growth was tested through tumor formation in nude mice. Results circ_0038718 was highly expressed in CC and could sponge miR-195-5p in cytoplasm. Silencing circ_0038718 suppressed the proliferative, migratory and invasive abilities of CC cells, while the promoting effect of high circ_0038718 expression on CC cells was reversed upon miR-195-5p over-expression. Axin2 was a downstream target of miR-195-5p and could regulate the Wnt/β-catenin signaling pathway. Axin2 expression was modulated by circ_0038718/miR-195-5p. Knockdown of Axin2 could also attenuate the promoting effect of high circ_0038718 expression on CC cell malignant progression, thus inhibiting tumor growth. Conclusion circ_0038718 is able to facilitate CC cell malignant progression via the miR-195-5p/Axin2 axis, which will provide a new idea for finding a novel targeted treatment of CC.

[Retracted] Circular RNA SMARCA5 Modulates Epithelial‐Mesenchymal Transformation, Proliferation, and Metastasis of Nasopharyngeal Carcinoma Cells via microRNA‐582‐3p/Phosphatase and Tensin Homolog Axis

“…Reaction (RT-qPCR) Detection. RT-qPCR was performed to detect miRNA and mRNA as mentioned above [20]. Te extraction of total RNA was from NPC tissues and cells adopting TRIzol kit (Invitrogen).…”

CERS6‐AS1 promotes cell proliferation and represses cell apoptosis in pancreatic cancer via miR‐195‐5p/WIPI2 axis

“…In this study, miR‐195‐5p was identified as the downstream miRNA of CERS6‐AS1 in PC. Previously, it was reported that miR‐195‐5p acts as an antioncogene in many types of cancer, including colon, 24 bladder, 25 lung, 26 and esophageal cancer 27 . For example, lncRNA BANCR was reported to promote the tumorigenesis of PC by targeting miR‐195‐5p and regulating the Wnt/β‐catenin signaling pathway 28 .…”

Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression

“…CircPVT1 has been proved to locate mainly in cytoplasm, which meant that it might act as microRNA sponges to regulate downstream gene expression. MiR-195 is dysregulated in several tumors, such as colon cancer [ 56 ], non-small lung cancer [ 57 ], laryngeal cancer [ 58 , 59 ], gastric cancer [ 60 ], cervical cancer [ 61 ], colorectal cancer [ 62 ], breast cancer [ 63 ], lung adenocarcinoma [ 64 ] and osteosarcoma [ 65 ]. Moreover, miR-195 has been demonstrated that play a significant role in PTC [ 66–69 ].…”