Circ_0072995 Promotes Proliferation and Invasion via Regulating miR-1253/EIF4A3 Signaling in HCC

Abstract

Background: Hepatocellular carcinoma (HCC) is a major threat for human health. This work aimed to determine the potential function of circ_0072995 in HCC progression and its molecular mechanism. Methods: qRT-PCR was conducted to analyze circ_0072995 expression. CCK8 and colony formation assays were utilized to detect cell proliferation. Transwell assay was performed to determine migration and invasion. Interactions among circ_0072995, miR-1253 and EIF4A3 (Eukaryotic Translation Initiation Factor 4A3) were predicted through bioinformatics methods and confirmed via luciferase reporter assay and RNA pulldown assay. Results: circ_0072995 expression was upregulated in HCC tissues. Circ_0072995 high level was associated with poor prognosis. Circ_0072995 knockdown impaired proliferation, migration, invasion and survival. MiR-1253 was sponged by circ_0072995 and targeted EIF4A3 directly. Circ_0072995 inhibited miR-1253 to upregulate EIF4A3 level. Conclusion: Circ_0072995 exerted tumorigenic roles to enhance HCC progression through activating EIF4A3 by sponging miR-1253.

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells

“…Crucially, we delineated that circEIF4A3 enhances CRC development by upregulating its parent gene, EIF4A3—a gene already established as a pivotal player in various cancers [ 44 , 45 ]. Previous studies have indicated that EIF4A3 is implicated in the circRNA/miRNA interaction network during tumor growth [ 46 ], and recent insights also suggest that circRNAs can modulate their host genes via the ceRNA mechanism [ 47 ]. To probe deeper, we explored the possibility of a ceRNA network involving EIF4A3 and circEIF4A3, predicting potential miRNAs that might interact with both entities using a bioinformatics approach.…”

Identification of a Novel Survival-Related circRNA–miRNA–mRNA Regulatory Network Related to Immune Infiltration in Liver Hepatocellular Carcinoma

“… Salmena et al (2011) first proposed the competitive endogenous RNA (ceRNA) hypothesis that circRNAs negatively regulate miRNA expression by interacting with miRNA response elements (MREs) and then regulate intracellular signaling pathways and downstream gene expression. Recently, several studies ( Yao et al, 2019 ; He et al, 2021 ) have revealed that circRNAs play a pivotal role in the occurrence and development of LIHC through ceRNA networks. In addition, circRNAs can be used as diagnostic or prognostic markers for LIHC patients ( Wang et al, 2019 ; Chen et al, 2020 ).…”