circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network

Abstract

Insufficient invasion of trophoblast cells is one of the important causes of preeclampsia (PE). Circular RNA (circRNA) has been proven to regulate the biological functions of trophoblast cells and mediate the progression of PE. The expression of circ_0085296, microRNA (miR)-942-5p, and thrombospondin 2 (THBS2) was detected by quantitative real-time PCR. In addition, the interaction between miR-942-5p and circ_0085296 or THBS2 was confirmed by dual-luciferase reporter assay and RIP assay. Our data showed that circ_0085296 was upregulated in the placental tissues of PE patients. Silenced circ_0085296 could enhance the proliferation, migration, invasion, and angiogenesis of HTR-8/SVneo cells. Besides, circ_0085296 was found to act as miR-942-5p sponge. Function analysis results suggested that miR-942-5p inhibitor reversed the positive regulation of circ_0085296 knockdown on the biological functions of HTR-8/SVneo cells. Moreover, THBS2 was a target of miR-942-5p, and its overexpression also reversed the promotion effect of miR-942-5p on the proliferation, migration, invasion, and angiogenesis of HTR-8/SVneo cells. Also, circ_0085296 was discovered to positively regulate THBS2 by sponging miR-942-5p. To sum up, our results revealed that circ_0085296 could inhibit trophoblast cells proliferation, migration, invasion, and angiogenesis by regulating miR-942-5p/THBS2, confirming that circ_0085296 might be a potential therapeutic target for PE.

Hsa_circ_0015382 is involved in the pathogenesis of preeclampsia by mediating THBS2 expression

“…Furthermore, THBS2 is a metastatic‐related gene in different cancers 24,36 . High expression of THBS2 was measured in PE samples, and circ‐AK2, 37 hsa_circ_0085296, 38 and circ‐OPHN1 39 could repress trophoblast proliferation, invasion, and migration through upregulating THBS2. In this research, THBS2 could be targeted by miR‐616‐3p.…”

Suppression of circular RNA serum and glucocorticoid‐induced kinase 1 elevates antioxidant molecules and angiogenesis in trophoblast cells to attenuate preeclampsia via microRNA‐508‐3p to target and restrain PUM homolog 1

“…44,45 For example, circ_0085296 inhibits the biological function of trophoblast cells through the miR-942-5p/THBS2 network and promotes the progression of PE. 46 A novel circular RNA, cir-cBRAP, can be used as an early predictor of PE. 47 In this study, circSGK1 expression was elevated in the placenta tissue of PE patients.…”

“…Recently, circRNA in PE has been frequently explored, and multiple circRNAs have been discovered, which are considered latent clinical diagnostic markers or therapeutic targets 44,45 . For example, circ_0085296 inhibits the biological function of trophoblast cells through the miR‐942‐5p/THBS2 network and promotes the progression of PE 46 . A novel circular RNA, circBRAP, can be used as an early predictor of PE 47 .…”