circ_C20orf11 enhances DDP resistance by inhibiting miR-527/YWHAZ through the promotion of extracellular vesicle-mediated macrophage M2 polarization in ovarian cancer

Yin, Jun; Huang, Haiyan; Long, Ying; Ma, Yan; Maerkeya, Kamalibaike; Dawuti, Reyanguli; Li, Li

doi:10.1080/15384047.2021.1959792

Cited by 15 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR‐527, an inflammatory disease‐related miRNA, was confirmed to be targeted by circ_0087199 in our work. MiR‐527 is a newly identified miRNA related to progression of cancers such as gastric cancer, 21 glioma 22 and ovarian cancer 23 . But there are almost no studies regarding its function in periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis

Wang,

Huo,

Cai

et al. 2024

Immunity Inflam & Disease

View full text Add to dashboard Cite

BackgroundCircular RNAs participate in the development of periodontitis. The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell (PDLC) injury during periodontitis.MethodsPDLCs were treated with lipopolysaccharides (LPS) to establish a periodontitis cell model. Quantitative real‐time polymerase chain reaction was used to detect the expression of circ_0087199, miR‐527, toll‐like receptor 4 (TLR4). Western blot analysis assay was performed to assess protein expression. Cell viability, proliferation, apoptosis and inflammation were investigated by cell counting kit‐8, EdU assay, flow cytometry and enzyme‐linked immunosorbent assay, respectively. Oxidative stress was evaluated by malondialdehyde assay kit and superoxide dismutase activity assay kit. The interaction between miR‐527 and circ_0087199 or TLR4 was confirmed by a dual‐luciferase reporter assay.ResultsCirc_0087199 and TLR4 expression levels were significantly increased, while miR‐527 was decreased in the periodontal ligament tissues of periodontitis patients and LPS‐stimulated PDLCs when compared with controls. LPS treatment inhibited cell viability and proliferation but induced cell apoptosis, inflammation and oxidative stress, whereas these effects were attenuated after circ_0087199 knockdown. Circ_0087199 bound to miR‐527 and regulated LPS‐caused PDLC damage by targeting miR‐527. Additionally, the overexpression of TLR4, a target gene of miR‐527, rescued miR‐527 mimic‐mediated effects on LPS‐treated PDLCs. Further, the regulation of circ_0087199 toward TLR4 involved miR‐527.ConclusionCirc_0087199 knockdown attenuated LPS‐induced apoptosis, inflammation and oxidative stress of PDLCs by regulating the miR‐527/TLR4 pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis

Wang,

Huo,

Cai

et al. 2024

Immunity Inflam & Disease

View full text Add to dashboard Cite

show abstract

“…Mechanistically, PKP3 upregulated by circIGF2BP3 combines with FXR1 to stabilize OTUB1 mRNA, which increases PD-L1 abundance by promoting its deubiquitination ( 39 ). A study on ovarian cancer has suggested that silencing of circ_C20orf11 suppresses extracellular vesicle (EV)-induced macrophage M2 polarization and enhances sensitivity to CDDP in vivo ( 40 ).…”

Section: Mechanisms Of Drug Resistancementioning

confidence: 99%

“…Table 6 displays the chemoresistance-related circRNAs in ovarian cancer. Both circ_C20orf11 and circulating exosomal circFoxp1 can confer CDDP resistance in ovarian cancer cells ( 40 , 125 ). In contrast, Cdr1as improves sensitivity to CDDP in ovarian cancer by modulating the miR-1270/SCAI axis ( 126 ).…”

Section: Circrnas and Drug Resistancementioning

confidence: 99%

The Role of Circular RNAs in the Drug Resistance of Cancers

et al. 2022

View full text Add to dashboard Cite

Cancer is a major threat to human health and longevity. Chemotherapy is an effective approach to inhibit cancer cell proliferation, but a growing number of cancer patients are prone to develop resistance to various chemotherapeutics, including platinum, paclitaxel, adriamycin, and 5-fluorouracil, among others. Significant progress has been made in the research and development of chemotherapeutic drugs over the last few decades, including targeted therapy drugs and immune checkpoint inhibitors; however, drug resistance still severely limits the application and efficacy of these drugs in cancer treatment. Recently, emerging studies have emphasized the role of circular RNAs (circRNAs) in the proliferation, migration, invasion, and especially chemoresistance of cancer cells by regulating the expression of related miRNAs and targeted genes. In this review, we comprehensively summarized the potential roles and mechanisms of circRNAs in cancer drug resistance including the efflux of drugs, apoptosis, intervention with the TME (tumor microenvironment), autophagy, and dysfunction of DNA damage repair, among others. Furthermore, we highlighted the potential value of circRNAs as new therapeutic targets and prognostic biomarkers for cancer.

show abstract

“…Moreover, it was demonstrated that circ_C20orf11 expression in OC is abnormally upregulated and enhances cisplatin (DDP)-based chemotherapy resistance. Circ_C20orf11 promotes EV-mediated M2 macrophage polarization by inhibiting miR-527/tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) signaling, in turn promoting DDP resistance in vitro and in vivo [ 153 ]. Overall, these findings show that circRNAs have a pivotal role in the regulation of the main steps of cancer progression.…”

Section: Circular-rnas In Ovarian Cancermentioning

confidence: 99%

Extracellular Vesicles-ceRNAs as Ovarian Cancer Biomarkers: Looking into circRNA-miRNA-mRNA Code

Cammarata

Barraco

Giusti

et al. 2022

Cancers

View full text Add to dashboard Cite

Ovarian cancer (OC) is one of the most lethal gynecologic malignancies in females worldwide. OC is frequently diagnosed at an advanced stage due to a lack of specific symptoms and effective screening tests, resulting in a poor prognosis for patients. Age, genetic alterations, and family history are the major risk factors for OC pathogenesis. Understanding the molecular mechanisms underlying OC progression, identifying new biomarkers for early detection, and discovering potential targets for new drugs are urgent needs. Liquid biopsy (LB), used for cancer detection and management, consists of a minimally invasive approach and practical alternative source to investigate tumor alterations by testing extracellular vesicles (EVs), circulating tumor cells, tumor-educated platelets, and cell-free nucleic acids. EVs are nanosize vesicles shuttling proteins, lipids, and nucleic acids, such as DNA, RNA, and non-coding RNAs (ncRNAs), that can induce phenotypic reprogramming of target cells. EVs are natural intercellular shuttles for ncRNAs, such as microRNAs (miRNAs) and circular-RNAs (circRNAs), known to have regulatory effects in OC. Here we focus on the involvement of circRNAs and miRNAs in OC cancer progression. The circRNA-microRNA-mRNA axis has been investigated with Circbank and miRwalk analysis, unraveling the intricate and detailed regulatory network created by EVs, ncRNAs, and mRNAs in OC.

show abstract

circ_C20orf11 enhances DDP resistance by inhibiting miR-527/YWHAZ through the promotion of extracellular vesicle-mediated macrophage M2 polarization in ovarian cancer

Cited by 15 publications

References 30 publications

Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis

Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis

The Role of Circular RNAs in the Drug Resistance of Cancers

Extracellular Vesicles-ceRNAs as Ovarian Cancer Biomarkers: Looking into circRNA-miRNA-mRNA Code

Contact Info

Product

Resources

About