Circadian and feeding cues integrate to drive rhythms of physiology in <i>Drosophila</i> insulin-producing cells

Barber, Annika F.; Erion, Renske; Holmes, Todd C.; Sehgal, Amita

doi:10.1101/gad.288258.116

Cited by 120 publications

(138 citation statements)

References 60 publications

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“…2). In Drosophila melanogaster, PI neurons are physiologically circadian rhythmic although by way of other clock neuron input 23,24 . These PI-like PER + /PDFneurons are not detected in Ae.…”

Section: Resultsmentioning

confidence: 99%

Circadian regulation of light-evoked attraction/avoidance in day- vs. night-biting mosquitoes

Baik

Nave

et al. 2019

Preprint

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Mosquitoes pose widespread threats to humans and other animals as disease vectors. Day-vs. night-biting mosquitoes occupy distinct time-of-day niches and exhibit very different innate temporal attraction/avoidance behavioral responses to light, yet little is known about their circuit or molecular mechanisms. Day-biting diurnal mosquitoes Aedes aegypti are attracted to light during the day regardless of spectra. In contrast, night-biting nocturnal mosquitoes Anopheles coluzzii avoid short, but not long wavelength light. Attraction/avoidance behavioral responses to light in both species change with time-of-day and show distinct sex and circuit differences. The basis of diurnal versus nocturnal behavior is driven by clock timing, which cycle anti-phase between day-biting versus night-biting mosquito species. Disruption of the circadian molecular clock severely interferes with light-evoked attraction/avoidance behavior in mosquitoes. In summary, attraction/avoidance mosquito behaviors are circadian and light regulated, which may be applied towards species specific control of harmful mosquitoes.

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Section: Resultsmentioning

confidence: 99%

Circadian regulation of light-evoked attraction/avoidance in day- vs. night-biting mosquitoes

Baik

Nave

et al. 2019

Preprint

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“…Drosophila insulin like peptide2 (DILP2) drives rhythmicity in the expression of lipase sxe2 transcript in the fat body. Rhythmic expression of sxe2 is disrupted in flies expressing dominant negative insulin receptor InR DN in the fat body [19]. Hence it is likely that perturbation on sxe2 oscillation plays a role in the reducing the triglyceride levels in flies expressing dilp2 -RNAi in IPCs and the flies expressing InR DN in the fat body.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, pars intercerebralis (PI) has been identified as an important clock output region that regulates activity rest rhythms [18] and the dorsal clock neurons (DN1) are physically and functionally connected to the insulin producing cells (IPCs) of the PI. Drosophila insulin like peptide 2 (DILP2) produced in IPCs is an identified clock output-signaling molecule that drives rhythmicity in the expression of lipase transcript in the fat body [19]. Furthermore, DILPs and insulin signaling play a pivotal role in feeding behavior [20], [21], [22].…”

Section: Introductionmentioning

confidence: 99%

Short Neuropeptide F regulates the starvation mediated enhanced locomotor Activity in Drosophila

Geo

Pathak

Kujur

et al. 2019

Preprint

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9 10 11 12 Running title: sNPF and DILP2 govern starvation-mediated hyperactivity and triglyceride 13 metabolism in fruit flies. 14 15 16 a AEK and HP contributed equally to this work.Abstract: 20 Circadian clock regulates various behavioral, metabolic and physiological processes to 21 occur at the most suitable time of the day. The most apparent behavioral outputs of the clock are 22 activity-rest rhythm and feeding. While clock regulates these two behaviors through 23 interconnected neuronal circuits, the precise pathway through which the clock coordinates 24 metabolism in accordance with the behavioral rhythms largely remains to be elucidated. This 25 study was aimed to elucidate the role of two circadian relevant metabolic neuropeptides, short 26 neuropeptide F (sNPF) and Drosophila Insulin Like Peptide 2 (DILP2) in triglyceride 27 metabolism, starvation-mediated hyperactivity, and food intake in Drosophila. The results 28 showed that snpf transcripts exhibit significant rhythmicity under 12:12 hour light-dark cycles 29 (LD) and constant darkness (DD). Knockdown of sNPF in sNPF producing neurons enhanced 30 the starvation-mediated hyperactivity in flies compared to the control. Further studies showed 31 that sNPF suppresses starvation-induced hyperactivity partly through the sNPF receptors 32 (sNPFR) expressed in insulin producing cells (IPC). Knockdown of Drosophila insulin like 33 peptide 2 (dilp2) in IPCs and reduced expression of the insulin receptors (InR) in the fat body 34 altered the starvation-mediated hyperactivity, lipid storage, and increased the food intake after 35 starvation. These results suggest a role for sNPF in modulating locomotor activity in accordance 36 with the nutrient availability and a novel role for circadian output molecule DILP2 in regulating 37 triglyceride metabolism and starvation-mediated behavioral changes in Drosophila. 38 39 triglyceride metabolism 40 41 42The circadian clock drives daily rhythms in a wide array of physiological and behavioral 43 processes by scheduling it at the appropriate time of the day. This inherent timekeeping system 44 coordinates the phase of two fundamental behavioral rhythms such as feeding and sleep/wake 45 cycles and it is believed to aid the organism to adapt with the cyclic external environmental 46 changes. The growing body of evidence indicates that the circadian clock not only mediates 47 optimal phasing of behavioral rhythmicity during 24 hour of a day but also has intimate ties with 48 metabolism [1]. While the clock orchestrates numerous metabolic pathways, the nutrient 49 availability and metabolic status can in turn feed back to impinge on the functioning of the 50 circadian clock [2], [3], [4], [5]. A large body of evidence accumulated over the past two 51 decades remarkably improved our understanding of the vital role of the clock in metabolism, 52 feeding behavior, and sleep wake cycle [6]. However, little is known about the precise 53 underlying pathways by which the circadian clock aids the organism to attain temporal 54 ...

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“…IPCs has been reported to be promoting wakefulness as the downstream pathway of sleep regulation by octopamine [16]. with IPCs and consequently generate a metabolism rhythm via the connection [28]. DN1 neuron has also been reported to be activated by cold through peripheral neuron and affect sleep onset.…”

Section: Discussionmentioning

confidence: 99%

Insulin Producing Cells Monitor the Cold and Compensate the Cold-Induced Sleep in Drosophila

Zhang

Cui

Zhu

2018

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Sleep is regulated by environmental factors including temperature, but the neural circuits that receive the sensory signal and mediate the regulation remain unclear. We examined how cold could influence Drosophila sleep patterns and its neural mechanism. The results showed that Drosophila has more sleep duration, less sleep latency and deeper sleep depth under cold condition. We identified the Insulin-producing Cells (IPCs) can be activated by cold, and receive the cold signal from the 11216 cold-sensing neuron without a direct synaptic connection. Elevation of IPCs' sensitivity to cold impairs the sleeppromoting effect of cold while blocking of IPCs enhance the effect mostly on sleep circadian, suggesting that the cold activated IPCs have a compensative role in sleep regulation. Our finding revealed a potential neural circuit that help maintain sleep circadian in detrimental environment and may give new insight to the complicated sleep regulation mechanism.

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Circadian and feeding cues integrate to drive rhythms of physiology in Drosophila insulin-producing cells

Cited by 120 publications

References 60 publications

Circadian regulation of light-evoked attraction/avoidance in day- vs. night-biting mosquitoes

Circadian regulation of light-evoked attraction/avoidance in day- vs. night-biting mosquitoes

Short Neuropeptide F regulates the starvation mediated enhanced locomotor Activity in Drosophila

Insulin Producing Cells Monitor the Cold and Compensate the Cold-Induced Sleep in Drosophila

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