CIRCADIAN CLOCK-ASSOCIATED1 Controls Resistance to Aphids by Altering Indole Glucosinolate Production

Lei, Jiaxin; Jayaprakasha, G.K.; Singh, Jashbir; Uckoo, Rammohan; Borrego, Eli J.; Finlayson, Scott A.; Kolomiets, Michael V.; Braam, Janet; Zhu‐Salzman, Keyan

doi:10.1104/pp.19.00676

Cited by 42 publications

(42 citation statements)

References 94 publications

(160 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, CCA1 enhanced plant resistance to aphids, consistent with a previous finding. 36 To begin to understand whether green peach aphids could cope with CCA1-mediated plant defense, we documented the progeny number of the CCA population when reared on CCA1-ox. Notably, the population size was approximately twice that of the COL population on CCA1-ox, although lower than the number of the COL population maintained on Col-0 ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To be consistent with our previous study, insect feeding experiments were performed under constant light. 36 Age-synchronized nymphs and plants in constant light were used for RNA extraction, bioassays and GST activity measures.…”

Section: Plant Growth and Insect Rearingmentioning

confidence: 99%

“…The VIGS vector targeting AtCYP79B2 and AtCYP79B3 was constructed according to Lei et al 36 For HIGS, aphid gene-specific PCR primers were designed to amplify 200-500 bp fragments using PerlPrimer software. Fragments were subjected to BLAST analysis against green peach aphid genome and our aphid transcriptome to avoid off-target silencing.…”

Section: Transient Expression Of Vigs and Higs Constructs In Arabidopsismentioning

confidence: 99%

“…43 2.7 Glucosinolate analysis Arabidopsis glucosinolates were extracted, measured and identified as described previously. 36 Glucosinolate extracts were run on a Waters® 1525 HPLC series (Milford, MA, USA) equipped with a 2996 photodiode array detector and Waters® 717 plus autosampler and separated on a an Xbridge C 18 column (RP 150 × 4.6 mm, 3.54 μm particle size) from Waters. Glucosinolate peaks were monitored at 229 nm.…”

Section: Rt-qpcr and Semi-quantitative Pcr Analysismentioning

confidence: 99%

“…Arabidopsis overexpressing CIRCADIAN CLOCK ASSOCIATED1 (CCA1-ox) displays aberrant circadian rhythm and exhibits enhanced resistance to green peach aphids via heightened production of indole glucosinolates when tested under constant light. 36 Here, we have attempted to understand an insect adaptive mechanism using the aphid CCA population maintained on CCA1-ox for over 40 generations. Through a combination of RNA-Seq, VIGS, HIGS and bioassay experiments, we identified two CYPs in green peach aphid that are critical to this process.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

Lei

Chen

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Overexpressing CIRCADIAN CLOCK ASSOCIATED1 in Arabidopsis thaliana (CCA1-ox) increases indole glucosinolate production and resistance to green peach aphid (Myzus persicae). Little is known of how aphids respond to this group of plant defense compounds or of the underlying molecular mechanism. Results: Aphids reared on CCA1-ox for over 40 generations (namely the CCA population) became less susceptible to CCA1-ox than aphids maintained on the wild-type Col-0 (namely the COL population). This elevated tolerance was transgenerational as it remained for at least eight generations after the CCA population was transferred to Col-0. Intriguingly, transcriptome analysis indicated that all differential cytochrome P450 monooxygenase genes (MpCYPs), primarily MpCYP4s, MpCYP380s and MpCYP6s, were more highly expressed in the CCA population. Application of a P450 inhibitor to the CCA population resulted in decreased aphid reproduction on CCA1-ox, which was not observed if aphids were reared on Col-0. When indole glucosinolate biosynthesis in CCA1-ox was blocked using virus-induced gene silencing, the effect of the P450 inhibitor on the CCA population was attenuated, affirming the essential role played by MpCYPs in counteracting the defense mechanism in CCA1-ox that is low or absent in Col-0. Furthermore, we used host-induced gene silencing to identify MpCYP380C6 and MpCYP380C9 that specifically facilitated the CCA population to cope with CCA1-mediated plant defense. Expression profiles revealed their possible contribution to the transgenerational tolerance observed in aphids. Conclusion: MpCYP380C6 and MpCYP380C9 in aphids play a crucial role in mitigating indole glucosinolate-mediated plant defense, and this effect is transgenerational.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Plant Growth and Insect Rearingmentioning

confidence: 99%

Section: Transient Expression Of Vigs and Higs Constructs In Arabidopsismentioning

confidence: 99%

Section: Rt-qpcr and Semi-quantitative Pcr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

Lei

Chen

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

show abstract

Performance of two species of whiteflies is unaffected by glucosinolate profile in Brassica plants

Qian

Pan

et al. 2021

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND While plant glucosinolates are known to impart resistance to many insects, their role in the interactions between plants and many phloem‐feeding insects such as whiteflies are poorly understood. The whitefly Bemisia tabaci complex comprises many cryptic species that differ in the ability to utilize Brassica plants. However, whether Brassica plants‐specific traits such as glucosinolates determine differences of whiteflies in colonizing Brassica plants remains in question. RESULTS We first observed performance of two whitefly species MEAM1 and Asia II 3, which differ obviously in their ability to colonize Brassica plants, on four cultivars of three Brassica species that vary in glucosinolate profile. We found that the life history characteristics of each of the two whitefly species seems to be only marginally affected by cultivar. We next used wild‐type Arabidopsis plants and mutants defective in glucosinolate biosynthesis or hydrolysis to explore the effects of glucosinolates on the whitefly. We found that fecundity and development of immature stages of neither of the two whitefly species differ significantly between wild‐type and mutants. CONCLUSION The data suggest that glucosinolates may have little effect on the oviposition by adults and the survival and development of immature stages of MEAM1 and Asia II 3 whiteflies. The marked differences in colonizing Brassica crops between the two whitefly species are likely due to plant traits other than glucosinolates. © 2021 Society of Chemical Industry.

show abstract

Recognition of CCA1 alternative protein isoforms during temperature acclimation

Zhang

Liu

et al. 2021

Plant Cell Rep

View full text Add to dashboard Cite

CIRCADIAN CLOCK-ASSOCIATED1 Controls Resistance to Aphids by Altering Indole Glucosinolate Production

Cited by 42 publications

References 94 publications

Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

Performance of two species of whiteflies is unaffected by glucosinolate profile in Brassica plants

Recognition of CCA1 alternative protein isoforms during temperature acclimation

Contact Info

Product

Resources

About