Circadian Phase Differences of Lyo- and Desmoenzymes

Autoradiographic study on the distribution of Bis-p-nitrophenylphosphate (BNPP), a specific inhibitor of carboxylesterases, in the rat

“…A critical approach to the differential inhibition method was published by CHOUDHURY and LUNDY in 1971 (24), further specified by CHOUDHURY in 1972 (26) who claimed that the degree of inhibition within histochemical sections and zymograms strongly depends on the substrate chosen. In 1967 YAP had focussed attention to other fundamental difficulties involved in quantitative histochemistry due to the loss of lyoenzymes during incubation time, to the increase of activity of desmoenzymes following fixation and to the changes of the lyo-desmoenzyrne proportions (39). The autoradiographic localization of a labelled specific and irreversible inhibitor should evade these problems.…”