Circadian physiology of metabolism

Panda, Satchidananda

doi:10.1126/science.aah4967

Cited by 812 publications

(706 citation statements)

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“…Genetic animal models of clock disruption as well as nutritional studies have shown that the circadian clockwork is associated with altered lipid homeostasis, fatty liver, and obesity (52). Although various clock mutant mouse models exhibit altered lipid metabolism (53), it is challenging to dissect the contribution of the circadian clock on lipid metabolism from the impact of fasting-feeding cycles and rest-activity. In vitro synchronized primary cells may therefore pave the way for future studies that link circadian clock defects to insulin resistance, obesity, and T2D, and constitute a powerful model for studying peripheral oscillators in other human tissues.…”

Section: Discussionmentioning

confidence: 99%

Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

Loizides‐Mangold

Perrin

Vandereycken

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Circadian clocks play an important role in lipid homeostasis, with impact on various metabolic diseases. Due to the central role of skeletal muscle in whole-body metabolism, we aimed at studying muscle lipid profiles in a temporal manner. Moreover, it has not been shown whether lipid oscillations in peripheral tissues are driven by diurnal cycles of rest-activity and food intake or are able to persist in vitro in a cell-autonomous manner. To address this, we investigated lipid profiles over 24 h in human skeletal muscle in vivo and in primary human myotubes cultured in vitro. Glycerolipids, glycerophospholipids, and sphingolipids exhibited diurnal oscillations, suggesting a widespread circadian impact on muscle lipid metabolism. Notably, peak levels of lipid accumulation were in phase coherence with core clock gene expression in vivo and in vitro. The percentage of oscillating lipid metabolites was comparable between muscle tissue and cultured myotubes, and temporal lipid profiles correlated with transcript profiles of genes implicated in their biosynthesis. Lipids enriched in the outer leaflet of the plasma membrane oscillated in a highly coordinated manner in vivo and in vitro. Lipid metabolite oscillations were strongly attenuated upon siRNA-mediated clock disruption in human primary myotubes. Taken together, our data suggest an essential role for endogenous cell-autonomous human skeletal muscle oscillators in regulating lipid metabolism independent of external synchronizers, such as physical activity or food intake.lipid metabolism | circadian clock | human skeletal muscle | human primary myotubes | lipidomics C ircadian oscillations are daily cycles in behavior and physiology that are driven by the existence of underlying intrinsic biological clocks with near 24-h periods. This anticipatory mechanism has evolved to ensure that all aspects of behavior and physiology, including metabolic pathways, are temporally coordinated with daily cycles of rest-activity and feeding to provide the organism with an adaptive advantage (1). In mammals, circadian oscillations are driven by a master pacemaker, located in the suprachiasmatic nucleus (SCN) of the hypothalamus, which orchestrates subsidiary oscillators in peripheral organs via neuronal, endocrine, and metabolic signaling pathways (2, 3).Large-scale gene expression datasets suggest that, in mammals, the vast majority of circadian-gene expression is highly organ-specific (4-6). Key metabolic functions in peripheral organs are subject to daily oscillations, such as carbohydrate and lipid metabolism by the liver, skeletal muscle, and endocrine pancreas (7).Skeletal muscle is a major contributor of whole-body metabolism and is the main site of glucose uptake in the postprandial state (8). Therefore, perturbations in glucose sensing and metabolism in skeletal muscle are strongly associated with insulin resistance in type 2 diabetes (T2D) (9). Recent data support a fundamental role for the circadian muscle clock in the regulation of glucose uptake, with a significant re...

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Section: Discussionmentioning

confidence: 99%

Lipidomics reveals diurnal lipid oscillations in human skeletal muscle persisting in cellular myotubes cultured in vitro

Loizides‐Mangold

Perrin

Vandereycken

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…65 El ritmo circadiano humano es controlado por el reloj neurológico en el núcleo supraquiasmático y los relojes periféricos ubicados en casi todas las células incluso las del sistema inmune.…”

Section: Ritmo Circadiano Sueño Y Respuesta Inmuneunclassified

Sueño y sistema inmune

Rico-Rosillo

Vega-Robledo

2018

RAM

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Este artículo debe citarse como: Rico-Rosillo MG, Vega-Robledo GB. Sueño y sistema inmune. Rev Alerg Mex. 2018;65(2):160-170 ORCID María Guadalupe Rico-Rosillo, ORCID: 0000-0002-3117-1617; Gloria Bertha Vega-Robledo, ORCID: 0000-0002-5816-1910 AbstractSleep is a process that occupies one third part of the life of the human being, and it is essential in order for the individual to be able to maintain body homeostasis. It emerges as an important regulator of the immune system since, during sleep, the necessary functions to maintain its balance are carried out. On the other hand, decreased sleep has deleterious eff ects that alter the metabolism and produce an increase in the secretion of C-reactive protein, interleukin (IL)-6 and tumor necrosis factor (TNF). These cytokines activate NF-κB; therefore, sleep disturbance can be a risk factor for the development of chronic infl ammatory and metabolic diseases. Pro-infl ammatory cytokines IL-1, IL-6 and TNF increase non-rapid eye movement sleep, whereas anti-infl ammatory cytokines such as IL-4 and IL-10 decrease it. Sleep can modify the immune system function by inducing changes in the hypothalamus-pituitary-adrenal axis and the sympathetic nervous system. In turn, the circadian rhythm of hormones such as cortisol and adrenaline, which have a nocturnal decrease, favors diff erent activities of the immune system. The purpose of the present review is to address diff erent aspects of sleep and their relationship with the immune system.

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“…These rhythms play an important role in disease processes and treatment effects, which has been largely overlooked in medicine [1][2][3]. They are generated at single cell level by molecular clocks consisting of interwoven feedback loops involving transcription/translation of 15 known specific "clock" genes including Bmal1, Clock, Per2 and Rev-erbα [4].…”

Section: Introductionmentioning

confidence: 99%

“…They are generated at single cell level by molecular clocks consisting of interwoven feedback loops involving transcription/translation of 15 known specific "clock" genes including Bmal1, Clock, Per2 and Rev-erbα [4]. The molecular clocks are coordinated at whole organism level by the suprachiasmatic nuclei (SCN), a hypothalamic pacemaker, which also helps circadian rhythms adjust to light-dark and other environmental 24-h cycles through the rhythmic control of rest-activity, body temperature, feeding, as well as cortisol and melatonin secretions [1][2][3][4]. Thus both, glucocorticoids and body temperature rhythms, reset molecular clocks and cellular circadian rhythms in vitro and in vivo [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%