Circadian protein CLOCK modulates regulatory B cell functions of nurses engaging day-night shift rotation

Wang, Qiaohong; Li, Linbo; Li, Chao; Cao, Huili; Zhou, Wei; Yang, Gui; Yang, Hui

doi:10.1016/j.cellsig.2022.110362

Cited by 11 publications

(14 citation statements)

References 26 publications

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“…We found that the amounts of CMIP were less in FA DCs compared to those in NC DCs. CMIP is the inducer of c-Maf [25], the transcription factor of Il10. The assessment of the expression of Maf and Il10 in DCs was in line with the amounts of CMIP, as shown by the present data.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway

Zeng,

Xu,

Liu

et al. 2024

Preprint

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The pathogenesis of food allergy (FA) is still not fully understood. Telomerases are involved in the regulation of immune responses. The aim of this study is to understand the contribution of telomerase reverse transcriptase (TERT) to the pathogenesis of FA. A murine FA model was established with ovalbumin as the specific antigen. This murine model was used to test the role of TERT in the regulation of dendritic cell (DC) immune tolerogenic functions. We observed that the Tert promoter was at demethylation status and the Tert expression was elevated in DCs of FA mice. The FA response was positively correlated with the Tert expression in DCs. Induction of Il10 expression in DCs was hindered by TERT. TERT hindered the immune tolerogenic functions of DCs. The immune tolerogenic functions of DC were restored by CpG by boosting the Tert promoter methylation. Administration of CpG promoted the therapeutic effects of allergen specific immunotherapy in FA mice. In conclusion, low levels of Il10 expression and high levels of Tert expression were observed in intestinal DCs of FA mice. CpG exposure restored the expression of Il10 and increased the therapeutic benefits of allergen-specific immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway

Zeng,

Xu,

Liu

et al. 2024

Preprint

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“…Such a phenomenon is consistent with previous reports, in which Wang et al found that CMIP played a critical role in the expression of IL10 in B cells. The circadian locomotor output cycles kaput (CLOCK) interacted with CMIP to cause degradation, which reduced IL10 expression in B cells [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

An association between elevated telomerase reverse transcriptase expression and the immune tolerance disruption of dendritic cells

Xu,

Mo,

Liao

et al. 2024

Cell Commun Signal

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Background To elucidate the mechanism of dysfunction of tolerogenic dendritic cells (DCs) is of significance. Telomerase involves the regulation of the cell fate and activities. The objective of this study is to investigate the role of telomerase reverse transcriptase (TERT) in regulating the tolerogenic feature of DCs. Methods The telomerase was assessed in DCs, which were collected from patients with allergic rhinitis (AR), healthy control (HC) subjects, and mice. RNAs were extracted from DCs, and analyzed by RNA sequencing (RNAseq), real-time quantitative RT-PCR, and Western blotting. Results The results showed that expression of TERT was higher in peripheral DCs of AR patients. The expression of IL10 in DCs was negatively correlated with the levels of TERT expression. Importantly, the levels of TERT mRNA in DCs were associated with the AR response in patients with AR. Endoplasmic reticulum (ER) stress promoted the expression of Tert in DCs. Sensitization with the ovalbumin-aluminum hydroxide protocol increased the expression of Tert in DCs by exacerbating ER stress. TERT interacting with c-Maf (the transcription factor of IL-10) inducing protein (CMIP) in DCs resulted in CMIP ubiquitination and degradation, and thus, suppressed the production of IL-10. Inhibition of Tert in DCs mitigated experimental AR. Conclusions Elevated amounts of TERT were detected in DCs of patients with AR. The tolerogenic feature of DCs was impacted by TERT. Inhibited TERT attenuated experimental AR.

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“…In line with exaggerated allergic DTH responses that are driven by T cells, Clock mutant mice also show enhanced IgE reactions to allergens ( 317 ). There is evidence that day-night-shift rotations attenuate the release of the anti-inflammatory cytokine IL-10 by B cells ( 419 ). This lack of immunologic regulation could be deleterious as the IL-10 releasing B cells of shift workers were unable to inhibit the proliferation of T cells ( 419 ).…”

Section: Potential Effects Of Shift Work On Skin and Immune Cellsmentioning

confidence: 99%

“…There is evidence that day-night-shift rotations attenuate the release of the anti-inflammatory cytokine IL-10 by B cells ( 419 ). This lack of immunologic regulation could be deleterious as the IL-10 releasing B cells of shift workers were unable to inhibit the proliferation of T cells ( 419 ). It could also be shown that CLOCK expression in peripheral B cells of shift workers was higher, which leads to a reduced expression of transforming growth factor beta (a cytokine mainly released by Tregs) ( 420 ).…”

Section: Potential Effects Of Shift Work On Skin and Immune Cellsmentioning

confidence: 99%

Potential effects of shift work on skin autoimmune diseases

2023

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Shift work is associated with systemic chronic inflammation, impaired host and tumor defense and dysregulated immune responses to harmless antigens such as allergens or auto-antigens. Thus, shift workers are at higher risk to develop a systemic autoimmune disease and circadian disruption with sleep impairment seem to be the key underlying mechanisms. Presumably, disturbances of the sleep-wake cycle also drive skin-specific autoimmune diseases, but epidemiological and experimental evidence so far is scarce. This review summarizes the effects of shift work, circadian misalignment, poor sleep, and the effect of potential hormonal mediators such as stress mediators or melatonin on skin barrier functions and on innate and adaptive skin immunity. Human studies as well as animal models were considered. We will also address advantages and potential pitfalls in animal models of shift work, and possible confounders that could drive skin autoimmune diseases in shift workers such as adverse lifestyle habits and psychosocial influences. Finally, we will outline feasible countermeasures that may reduce the risk of systemic and skin autoimmunity in shift workers, as well as treatment options and highlight outstanding questions that should be addressed in future studies.

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Circadian protein CLOCK modulates regulatory B cell functions of nurses engaging day-night shift rotation

Cited by 11 publications

References 26 publications

Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway

Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway

An association between elevated telomerase reverse transcriptase expression and the immune tolerance disruption of dendritic cells

Potential effects of shift work on skin autoimmune diseases

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