Circadian regulation of phosphodiesterase 6 genes in zebrafish differs between cones and rods: Implications for photopic and scotopic vision

Abalo, Xesús M.; Lagman, David; Heras, Gabriel; Pozo, Ana del; Eggert, Joel; Larhammar, Dan

doi:10.1016/j.visres.2019.11.001

Cited by 17 publications

(11 citation statements)

References 34 publications

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“…Clusters were removed if they contained an abnormally low number of average genes per cell, did not express rbpms2b, or expressed genes present in contaminant cell types. Examples of such genes include rlbp1a and apoeb for Muller glia (Bernardos et al, 2007), vsx1 for bipolar cells (Vitorino et al, 2009), gad1 and gad2 for amacrine cells (Sandell et al, 1994), pde6 for photoreceptors (Abalo et al, 2020), and cldn19 for endothelial cells (Kolosov et al, 2013). A total of 15,909 cells corresponding to these cell classes were removed.…”

Section: Identification Of Rgcs and Filtering Contaminant Classesmentioning

confidence: 99%

Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

Kölsch

Hahn

Sappington³

et al. 2021

Neuron

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Retinal ganglion cells (RGCs) form an array of feature detectors, which convey visual information to central brain regions. Characterizing RGC diversity is required to understand the logic of the underlying functional segregation. Using single-cell transcriptomics, we systematically classified RGCs in adult and larval zebrafish, thereby identifying marker genes for at least 33 stable and transient cell types. We used this dataset to engineer transgenic driver lines, enabling experimental access to specific RGC types. Strikingly, expression of one or few transcription factors often predicts dendrite morphologies and axonal projections to specific tectal layers and extratectal targets. In vivo calcium imaging revealed that molecularly defined RGCs exhibit highly specific functional tuning. Finally, chemogenetic ablation of eomesa + RGCs, which comprise melanopsin-expressing types with projections to a small subset of central targets, selectively impaired phototaxis. Together, our study establishes a framework for systematically studying the functional architecture of the visual system.

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Section: Identification Of Rgcs and Filtering Contaminant Classesmentioning

confidence: 99%

Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

Kölsch

Hahn

Sappington³

et al. 2021

Neuron

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“…Melatonin regulates various rhythms of zebrafish, including circadian rhythms . Our results showed that Cd and Cu exposure led to a significant decrease in melatonin content in zebrafish larvae (Figure O), which may be one of the causes of disruptive circadian rhythms after metal exposure. − Visual function and circadian rhythm can also affect each other, and any change in them can affect the locomotor and feeding activity of zebrafish. ,− However, the effects of metals on visual function, circadian rhythm, and behavior have not been thoroughly studied. , …”

Section: Resultsmentioning

confidence: 91%

Elucidating the Differences in Metal Toxicity by Quantitative Adverse Outcome Pathways

Yang

Zeng

Gao

et al. 2022

Environ. Sci. Technol.

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Numerous studies have reported that the toxicity differences among metals are widespread; however, little is known about the mechanism of differences in metal toxicity to aquatic organisms due to the lack of quantitative understanding of their adverse outcome pathway. Here, we investigated the effects of Cd and Cu on bioaccumulation, gene expression, physiological responses, and apical effects in zebrafish larvae. RNA sequencing was conducted to provide supplementary mechanistic information for the effects of Cd and Cu exposure. On this basis, we proposed a quantitative adverse outcome pathway (qAOP) suitable for metal risk assessment of aquatic organisms. Our work provides a mechanistic explanation for the differences in metal toxicity where the strong bioaccumulation of Cu enables the newly accumulated Cu to reach the threshold that causes different adverse effects faster than Cd in zebrafish larvae, resulting in a higher toxicity of Cu than that of Cd. Furthermore, we proposed a parameter C IT/BCF (the ratio of internal threshold concentration and bioaccumulation factor) that helps to understand the toxicity differences by combining the information of bioaccumulation and internal threshold of adverse effects. This work demonstrated that qAOP is an effective quantitative tool for understanding the toxicity mechanism and highlight the importance of toxicokinetics and toxicodynamics at different biological levels in determining the metal toxicity.

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Section: Identification Of Rgcs and Filtering Contaminant Classes: Rgmentioning

confidence: 99%

Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

Kölsch

Hahn

Sappington³

et al. 2020

Preprint

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SummaryRetinal ganglion cells (RGCs) form an array of feature detectors, which convey visual information to central brain regions. Characterizing RGC diversity is required to understand the logic of the underlying functional segregation. Using single-cell transcriptomics, we systematically classified RGCs in adult and larval zebrafish, thereby identifying marker genes for at least 33 stable and transient cell types. We used this dataset to engineer transgenic driver lines, enabling experimental access to specific RGC types. Strikingly, expression of one or few transcription factors often predicts dendrite morphologies and axonal projections to specific tectal layers and extratectal targets. In vivo calcium imaging revealed that molecularly defined RGCs exhibit highly specific functional tuning. Finally, chemogenetic ablation of eomesa+ RGCs, which comprise melanopsin-expressing types with projections to a small subset of central targets, selectively impaired phototaxis. Together, our study establishes a framework for systematically studying the functional architecture of the visual system.

show abstract

Circadian regulation of phosphodiesterase 6 genes in zebrafish differs between cones and rods: Implications for photopic and scotopic vision

Cited by 17 publications

References 34 publications

Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

Elucidating the Differences in Metal Toxicity by Quantitative Adverse Outcome Pathways

Molecular classification of zebrafish retinal ganglion cells links genes to cell types to behavior

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