Circadian Rhythm and Circulating Cell-Free DNA Release on Healthy Subjects

Poulet, Geoffroy; Hulot, Jean‐Sébastien; Blanchard, Anne; Bergerot, Damien; Xiao, Wenjin; Ginot, Frédéric; Boutonnet-Rodat, Audrey; Beinse, Guillaume; Geromel, Vanna; Pellegrina, Laurence; Azizi, Michel; Laurent‐Puig, Pierre; Benhaïm, Léonor; Taly, Valérie

doi:10.21203/rs.3.rs-1270021/v1

Cited by 3 publications

(2 citation statements)

References 32 publications

(36 reference statements)

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“…A unique peak was observed in each of the male and female groups, although the inhomogeneity between the samples in the male group raised concerns about the significance of these differences, which need to be confirmed in larger groups. There is not really much information in the literature regarding structural ccfDNA differences between genders, although levels are reported to be higher in men [59]. We can only speculate that observed differences could be attributed to the Y chromosome.…”

Section: Discussionmentioning

confidence: 65%

Label-Free Human Disease Characterization through Circulating Cell-Free DNA Analysis Using Raman Spectroscopy

Papadakis

Cheimonidi

Παναγοπούλου

et al. 2023

IJMS

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Circulating cell-free DNA (ccfDNA) is a liquid biopsy biomaterial attracting significant attention for the implementation of precision medicine diagnostics. Deeper knowledge related to its structure and biology would enable the development of such applications. In this study, we employed Raman spectroscopy to unravel the biomolecular profile of human ccfDNA in health and disease. We established reference Raman spectra of ccfDNA samples from healthy males and females with different conditions, including cancer and diabetes, extracting information about their chemical composition. Comparative observations showed a distinct spectral pattern in ccfDNA from breast cancer patients taking neoadjuvant therapy. Raman analysis of ccfDNA from healthy, prediabetic, and diabetic males uncovered some differences in their biomolecular fingerprints. We also studied ccfDNA released from human benign and cancer cell lines and compared it to their respective gDNA, confirming it mirrors its cellular origin. Overall, we explored for the first time Raman spectroscopy in the study of ccfDNA and provided spectra of samples from different sources. Our findings introduce Raman spectroscopy as a new approach to implementing liquid biopsy diagnostics worthy of further elaboration.

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Section: Discussionmentioning

confidence: 65%

Label-Free Human Disease Characterization through Circulating Cell-Free DNA Analysis Using Raman Spectroscopy

Papadakis

Cheimonidi

Παναγοπούλου

et al. 2023

IJMS

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“…Additionally, the analysis of genetic mutations, single nucleotide polymorphisms (SNPs), and other genomic alterations in ccfDNA has significant implications in personalized medicine. For instance, the identification of specific mutations in ccfDNA from a cancer patient can guide the selection of targeted therapies, tailored to the genetic makeup of the individual's tumor [21].…”

Section: Methylation Patterns and Genetic Signaturesmentioning

confidence: 99%

Exploring the Role of Circulating Cell-Free DNA in Disease Diagnosis and Therapy

Odah

2024

Preprint

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Recent advancements in molecular diagnostics have highlighted the potential of circulating cell-free DNA (ccfDNA) as a biomarker in various diseases. This study aims to explore the role of ccfDNA in the early diagnosis, prognosis, and therapeutic monitoring of diseases, with a focus on oncology. We conducted a comprehensive review and analysis of current literature, combined with experimental approaches involving advanced genomic sequencing and bioinformatics techniques. Our research systematically characterizes the molecular composition of ccfDNA, including its origins, size distribution, and methylation patterns, across different disease states. Furthermore, we investigate the dynamics of ccfDNA release and clearance in the bloodstream, elucidating mechanisms that could influence its diagnostic accuracy. In the context of cancer, we analyze the correlation between tumor-derived ccfDNA and disease progression, response to therapy, and overall survival. Our findings indicate that ccfDNA holds promise as a non-invasive biomarker for early disease detection, real-time monitoring of treatment efficacy, and potentially, predicting relapse. This study contributes to the growing field of liquid biopsies and opens new avenues for personalized medicine, emphasizing the need for standardized protocols in ccfDNA analysis to maximize its clinical utility. The implications of our findings extend beyond oncology, suggesting potential applications of ccfDNA in other pathological conditions.

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The rising tide of cell-free DNA profiling: from snapshot to temporal genome analysis

Bronkhorst

Ungerer

Oberhofer

et al. 2022

Journal of Laboratory Medicine

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Genomes of diverse origins are continuously shed into human body fluids in the form of fragmented cell-free DNA (cfDNA). These molecules maintain the genetic and epigenetic codes of their originating source, and often carry additional layers of unique information in newly discovered physico-chemical features. Characterization of cfDNA thus presents the opportunity to non-invasively reconstruct major parts of the host- and metagenome in silico. Data from a single specimen can be leveraged to detect a broad range of disease-specific signatures and has already enabled the development of many pioneering diagnostic tests. Moreover, data from serial sampling may allow unparalleled mapping of the scantily explored landscape of temporal genomic changes as it relates to various changes in different physiological and pathological states of individuals. In this review, we explore how this vast dimension of biological information accessible through cfDNA analysis is being tapped towards the development of increasingly powerful molecular assays and how it is shaping emerging technologies. We also discuss how this departure from traditional paradigms of snapshot genetic testing may pave the way for an onrush of new and exciting discoveries in human biology.

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Circadian Rhythm and Circulating Cell-Free DNA Release on Healthy Subjects

Cited by 3 publications

References 32 publications

Label-Free Human Disease Characterization through Circulating Cell-Free DNA Analysis Using Raman Spectroscopy

Label-Free Human Disease Characterization through Circulating Cell-Free DNA Analysis Using Raman Spectroscopy

Exploring the Role of Circulating Cell-Free DNA in Disease Diagnosis and Therapy

The rising tide of cell-free DNA profiling: from snapshot to temporal genome analysis

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