Circadian rhythmicity in the methylation of 5-hydroxy-indoles and norepinephrine in the pineal gland of 10 day old rats

Balemans, M. G. M.; Bary, F. A. M.; Legerstee, W. C.; Noordegraaf, E. M.

doi:10.1007/bf01579076

Cited by 4 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The existence of a circadian rhythm in the synthesis of proteins, nucleic acids, 5-Hydroxytryptamine, melatonin, Hydroxyindole-Omethyhransferase and N-acetyhransferase in the pineal is well docu-mented (Quay, 1963;Balemans et al, 1978;Binkley, 1983). Therefore a relationship between this biological clock (Binkley et al, 1978;Binkley, 1983) and rhythmicity of adrenocortical hormones may be expected.…”

Section: Discussionmentioning

confidence: 93%

Effect of neonatal pinealectomy on circadian periodicity of adrenocortical activity

Kaplanski¹,

Ronen²

1986

J. Neural Transmission

View full text Add to dashboard Cite

Newborn male and female rats aged between 24 and 48 hours were pinealectomized (Px) or sham-operated (Sh). Animals were sacrificed at 45 and 60 days of age, at the trough and peak of the adrenocortical diurnal cycle. Blood was collected for corticosterone determinations and the adrenal glands weighed. Adrenal gland weight was greater at 45 days (p less than 0.01) in Px rats than in Sh rats. This difference in adrenal gland weight disappeared in the group sacrificed 60 days post-operatively. On the other hand, the diurnal plasma rhythm of corticosterone remained intact in male and female rats with or without pineal at 45 and 60 days. Furthermore, the trough and peak plasma corticosterone levels were not different in Px 45 and 60 days post-operatively. These data do not support the assumption of the important role of the pineal in the newborn rat for the maintenance of a normal circadian rhythm of the pituitary-adrenal system.

show abstract

Section: Discussionmentioning

confidence: 93%

Effect of neonatal pinealectomy on circadian periodicity of adrenocortical activity

Kaplanski¹,

Ronen²

1986

J. Neural Transmission

View full text Add to dashboard Cite

show abstract

“…From these results, it also appears that the methylation capacity (i.e., HIOMT activity) was different with regard to the various hydroxyindoles (e.g., more or less high for [3H]HW and ['HIHL and low for [3H]HT and ['HIHA). These differences might result from the existence of several isoenzymes of HIOMT as suggested previously [mammals, Balemans et al, 1978;fish, Birks and Ewing, 19811. Starting spot. The radioactivity was low with regard to that found in birds in similar experimental conditions [14% in the pike, 30 to 60% in the parakeet, Juillard et al, 1983;60 to 80% in the pigeon, Voisin et al, 19831.…”

Section: Uptake and Metabolism Of [3h]htmentioning

confidence: 60%

“…These differences might result from the existence of several isoenzymes of HIOMT as suggested previously [mammals, Balemans et al, 1978;fish, Birks and Ewing, 19811. Starting spot. The radioactivity was low with regard to that found in birds in similar experimental conditions [14% in the pike, 30 to 60% in the parakeet, Juillard et al, 1983; 60 to 80% in the pigeon, Voisin et al, 19831. According to Collin et al [1982], it might correspond to [3H]indoles linked to proteins and which could not therefore migrate on the TLC plates.…”

Section: Uptake and Metabolism Of [3h]htmentioning

confidence: 84%

“…5-Methoxytryptamine has been identified in pineal glands of mammals [Beck et al, 1981;Balemans, 1981;Pevet, 19831 and of the pike [Falcbn, 19841. It is generally accepted that MT is synthesized in the pineal after methylation of HT and/or decarboxylation of MW [Balemans et al, 19831. However, MT can also be synthetized after deacetylation of aMT. The existence of aryl-acylamidases (aryl-acylamide amidohydrolase, EC 3.5.1.13) has been demonstrated in a number of mammalian tissues, including brain [Rogawski et al, 1979;Beck and Jonsson, 1981;Hsu, 19841.…”

Section: Uptake and Metabolism Of [3h]amtmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Uptake and Metabolism of [³H]Indole Compounds in the Pineal Organ of the Pike. I. A Radiochromatographic Study

Falcón

Balemans

Benthem

et al. 1985

Journal of Pineal Research

View full text Add to dashboard Cite

Thin layer chromatography analysis of [3H]serotonin and [3H]melatonin metabolites synthetized in vitro by the pineal organ of the pike was performed. After a 10-min pulse, [3H]serotonin was mainly converted into [3H]-5-hydroxyindoleacetic acid (37%), [3H]-5-hydroxytryptophan and [3H]-5-methoxytryptophan (12 to 14%), and [3H]-5-hydroxytryptophol and [3H]-5-methoxytryptophol (3.5 and 9%) at the onset of darkness. When the pulse was followed by postincubations (in a cold medium) of increasing duration (15, 30, and 60 min), it appeared that the amount of [3H]-5-hydroxyindoleacetic acid decreased, that of [3H]-5-hydroxytryptophol decreased faster than that of [3H]-5-methoxytryptophol, and the amounts of [3H]-5-hydroxy- and [3H]-5-methoxytryptophan increased. [3H]-N-acetylserotonin, [3H]melatonin, and [3H]-5-methoxytryptamine were found in very low amounts. At the beginning of the photophase or at the onset of darkness, the uptake and metabolism of [3H]melatonin (after a 10-min pulse followed by a 10-min incubation in cold medium) resulted mainly in the formation of [3H]-5-methoxytryptophol (23 to 43%) and of [3H]-5-methoxytryptamine (6 to 12%). These results show that the pike pineal organ can synthesize all indoles that are known in the pineal gland of higher vertebrates. Usual, but also unusual, pathways of the indole metabolism were found that will need further clarification. Among these are the possible carboxylation of serotonin and deacetylation of melatonin (leading to the synthesis of 5-methoxytryptophol). Altogether, the results obtained suggest that the indole metabolism might be more complex than what has already been described in vertebrates.

show abstract

Day and night rhythms in the methylation of N-acetylserotonin/5-hydroxytryptophol in the pineal gland of male rats of different ages

Balemans

Legerstee

Benthem

1979

J. Neural Transmission

View full text Add to dashboard Cite

Next to a night rhythm of the methylation of N-acetylserotonin/5-hydroxytryptophol the presence of a daytime rhythm could also be established. Rhythmicity was studied in May during the night and in June during daytime in 21, 42 and 70 days old male Wistar rats. In 21 days old rats, moderate HIOMT activity was observed from 12 p.m.--4 a.m. In rats aged 42 and 70 days HIOMT activity was increased showing a peak at 4 a.m. In September this night maximum is observed at 12 p.m. in rats, aged 42 days. This points to the presence of a seasonal change in HIOMT rhythmicity during the night. During daytime a moderate HIOMT activity is present, which reaches a maximum at 2 p.m. in the 21 and 42 day old animals, while in the adult 70 days old rats activity starts to increase at 2 p.m. probably reaching a maximum at 6 p.m.

show abstract

Circadian rhythmicity in the methylation of 5-hydroxy-indoles and norepinephrine in the pineal gland of 10 day old rats

Cited by 4 publications

References 28 publications

Effect of neonatal pinealectomy on circadian periodicity of adrenocortical activity

Effect of neonatal pinealectomy on circadian periodicity of adrenocortical activity

In Vitro Uptake and Metabolism of [³H]Indole Compounds in the Pineal Organ of the Pike. I. A Radiochromatographic Study

Day and night rhythms in the methylation of N-acetylserotonin/5-hydroxytryptophol in the pineal gland of male rats of different ages

Contact Info

Product

Resources

About

Circadian rhythmicity in the methylation of 5-hydroxy-indoles and norepinephrine in the pineal gland of 10 day old rats

Cited by 4 publications

References 28 publications

Effect of neonatal pinealectomy on circadian periodicity of adrenocortical activity

Effect of neonatal pinealectomy on circadian periodicity of adrenocortical activity

In Vitro Uptake and Metabolism of [3H]Indole Compounds in the Pineal Organ of the Pike. I. A Radiochromatographic Study

Day and night rhythms in the methylation of N-acetylserotonin/5-hydroxytryptophol in the pineal gland of male rats of different ages

Contact Info

Product

Resources

About

In Vitro Uptake and Metabolism of [³H]Indole Compounds in the Pineal Organ of the Pike. I. A Radiochromatographic Study