Circadian Time Effects on NB-UVB–Induced Erythema in Human Skin In Vivo

Nikkola, Veera; Grönroos, Mari; Huotari-Orava, Riitta; Kautiainen, Hannu; Ylianttila, Lasse; Karppinen, Toni; Partonen, Timo; Snellman, Erna

doi:10.1016/j.jid.2017.08.016

Cited by 27 publications

(21 citation statements)

References 15 publications

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“…Chronic exposure to UVB irradiation injures skin, causing premature wrinkles, dryness, thinning, and increased pigmentation [6,17,18], as well as acute inflammation such as edema and erythema [19][20][21][22]. Most of these phenomena occur due to damage to the dermis, where fibroblast cells are located.…”

Section: Introductionmentioning

confidence: 99%

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Lim

Jeong

Park

et al. 2020

IJMS

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UVB irradiation can induce generation of reactive oxygen species (ROS) that cause skin aging or pigmentation. Lactobacillus acidophilus is a well-known probiotic strain that regulates skin health through antimicrobial peptides and organic products produced by metabolism and through immune responses. In this study, we investigated the antioxidative, antiwrinkle, and antimelanogenesis effects of tyndallized Lactobacillus acidophilus KCCM12625P (AL). To analyze the effects of AL on UV irradiation-induced skin wrinkle formation in vitro, human keratinocytes and human dermal fibroblasts were exposed to UVB. Subsequent treatment with AL induced antiwrinkle effects by regulating wrinkle-related genes such as matrix metalloproteinases (MMPs), SIRT-1, and type 1 procollagen (COL1AL). In addition, Western blotting assays confirmed that regulation of MMPs by AL in keratinocytes was due to regulation of the AP-1 signaling pathway. Furthermore, we confirmed the ability of AL to regulate melanogenesis in B16F10 murine melanoma cells treated with α-melanocyte-stimulating hormone (α-MSH). In particular, AL reduced the mRNA expression of melanogenesis-related genes such as tyrosinase, TYRP-1, and TYRP-2. Finally, we used Western blotting assays to confirm that the antimelanogenesis role of AL was due to its regulation of the cyclic adenosine monophosphate (cAMP) signaling pathway. Collectively, these results indicate that AL has an antiwrinkle activity in damaged skin and can inhibit melanogenesis. Thus, AL should be considered an important substance for potential use in anti-aging drugs or cosmetics.

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Section: Introductionmentioning

confidence: 99%

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Lim

Jeong

Park

et al. 2020

IJMS

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“…For example, p53 activity is controlled by BMAL1 in pancreatic cancer 30 , and p53 negatively regulates Per2 expression 22 . Another group demonstrated that UV triggers time-dependent erythemal responses in human skin cells through CRY2 and p53 31 , 32 . These observations imply that the molecular clock responds to genotoxic stress by resetting its phases and simultaneously regulating stress response pathways.…”

Section: Discussionmentioning

confidence: 99%

Cooperative interaction among BMAL1, HSF1, and p53 protects mammalian cells from UV stress

et al. 2018

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The circadian clock allows physiological systems to adapt to their changing environment by synchronizing their timings in response to external stimuli. Previously, we reported clock-controlled adaptive responses to heat-shock and oxidative stress and showed how the circadian clock interacts with BMAL1 and HSF1. Here, we present a similar clock-controlled adaptation to UV damage. In response to UV irradiation, HSF1 and tumor suppressor p53 regulate the expression of the clock gene Per2 in a time-dependent manner. UV irradiation first activates the HSF1 pathway, which subsequently activates the p53 pathway. Importantly, BMAL1 regulates both HSF1 and p53 through the BMAL1–HSF1 interaction to synchronize the cellular clock. Based on these findings and transcriptome analysis, we propose that the circadian clock protects cells against the UV stress through sequential and hierarchical interactions between the circadian clock, the heat shock response, and a tumor suppressive mechanism.

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“…Circadian clock proteins and genes have been shown to regulate cellular responses after UVB exposure: in mice, sensitivity to UVB‐induced DNA damage in the epidermal skin is a process that is dependent on ARNTL as a function of the time of day . Earlier, we showed the effect on circadian timing in UVB‐induced erythema response in human epidermal/dermal skin . However, the effect of UVB radiation on clock genes is not fully understood.…”

Section: Introductionmentioning

confidence: 98%

Ultraviolet B radiation modifies circadian time in epidermal skin and in subcutaneous adipose tissue

Nikkola

Miettinen

Karisola

et al. 2018

Photoderm Photoimm Photomed

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Ultraviolet radiation (UVR) reaching the skin is a carcinogen, inducing immunosuppression and DNA mutations, 1 and the wavelengths of ultraviolet light B (UVB) represent a biologically very active part of it. 2 The chromophores transduct the electromagnetic energy of UVB into neural, chemical, and hormonal signals to produce rapid (neural) or slow (humoral or immune) responses at the local and systemic levels. 3,4 Skin irradiated by UVB can activate both the central and local hypothalamic-pituitary-adrenal (HPA) axis to turn on homeostatic responses and deactivate local and systemic damage. 4,5 Locally UVB irradiation of the skin leads to activation of pro-opiomelanocortin (POMC) and corticotropin-releasing hormone (CRH) in epidermal and dermal skin, produces cutaneous POMC peptides and activates the corresponding receptors that are important regulators on the pigmentary and the inflammatory reactions. 6-8 In addition, keratinocytes stimulated by UVB can, as a rapid effect, secrete, for example, Summary Background: Recent findings suggest that circadian time regulates cellular functions in the skin and may affect protection against ultraviolet radiation (UVR). It is not known, however, whether UVR through skin directly affects the expression of circadian genes. We investigated the effect of ultraviolet B (UVB) exposure on cryptochrome circadian clock 1 (CRY1), cryptochrome circadian clock 2 (CRY2), and circadian associated repressor of transcription (CIART) genes.Methods: Healthy volunteers (n = 12) were exposed to narrow-band UVB radiation of four standard erythemal dose (SED). Epidermal/dermal and subcutaneous adipose tissue samples were obtained by punch biopsies from irradiated and non-irradiated skin 10 cm away from the irradiated site 24 hours after UVB exposure. Gene expression of CRY1, CRY2, and CIART was measured using RT-PCR (TaqMan). Results: Ultraviolet B radiation affected mRNA expression in the epidermal/dermal skin and in the subcutaneous adipose tissue. It down-regulated expression of CRY2 gene in the epidermal/dermal skin, whereas it up-regulated expression of CRY1 and CIART genes in the subcutaneous adipose tissue. Conclusion:We showed for the first time that UVB radiation affects expression of circadian genes in the subcutaneous adipose tissue. Further studies are warranted to understand the mechanisms in detail.

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Circadian Time Effects on NB-UVB–Induced Erythema in Human Skin In Vivo

Abstract: Similar itch and nociceptive sensations evoked by punctate cutaneous application of capsa-icin, histamine and cowhage. Pain 2009;144: 66e75.

Cited by 27 publications

References 15 publications

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Cooperative interaction among BMAL1, HSF1, and p53 protects mammalian cells from UV stress

Ultraviolet B radiation modifies circadian time in epidermal skin and in subcutaneous adipose tissue

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