Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs

Kanai, Kazuaki; Hino, Mariko; Hori, Yuichi; Nakao, Ruriko; Hoshi, Fumio; Itoh, Naoyuki; Higuchi, Seiichi

doi:10.4142/jvs.2008.9.4.421

Cited by 19 publications

(22 citation statements)

References 16 publications

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“…In addition, CgA in saliva has been found to be increased in early morning samples (7:00 am) in humans [13, 77, 78]. In our study, there was no significant difference in the concentrations of plasma CST, VS and saliva CST between time of day, which is in agreement with a previous study that used an ELISA for human CgA 344–374 amino acid sequence for saliva samples in dogs [3]. On the other hand, a circadian variation in CgA has been found in plasma and saliva in humans [13, 79].…”

Section: Discussionsupporting

confidence: 92%

Catestatin and vasostatin concentrations in healthy dogs

et al. 2017

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BackgroundThe neuroendocrine glycoprotein chromogranin A is a useful biomarker in humans for neuroendocrine tumors and stress. Chromogranin A can be measured in both blood and saliva. The objective of this study was to investigate concentrations of and correlation between the chromogranin A epitopes catestatin and vasostatin in healthy dogs accustomed to the sample collection procedures. Blood and saliva samples were collected from 10 research Beagle dogs twice daily for 5 consecutive days, and from 33 privately-owned blood donor dogs in association with 50 different blood donation occasions. All dogs were familiar with sample collection procedures. During each sampling, stress behavior was scored by the same observer using a visual analog scale (VAS) and serum cortisol concentrations. Catestatin and vasostatin were analyzed using radioimmunoassays for dogs.ResultsThe dogs showed minimal stress behavior during both saliva sampling and blood sampling as monitored by VAS scores and serum cortisol concentrations. Few and insufficient saliva volumes were obtained and therefore only catestatin could be analyzed. Catestatin concentrations differed significantly and did not correlate significantly with vasostatin concentrations (P < 0.0001). Age, gender, breed, and time of sample collection did not significantly affect concentrations of plasma catestatin, vasostatin, and saliva catestatin.ConclusionsThe normal ranges of plasma catestatin (0.53–0.98 nmol/l), vasostatin (0.11–1.30 nmol/l), and saliva catestatin (0.31–1.03 nmol/l) concentrations in healthy dogs accustomed to the sampling procedures were determined. Separate interpretation of the different chromogranin A epitopes from either saliva or plasma is recommended.

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Section: Discussionsupporting

confidence: 92%

Catestatin and vasostatin concentrations in healthy dogs

et al. 2017

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“…A significant advantage with respect to other salivary markers of stress, such as cortisol (Ruis et al, 1997) alphaamylase (Harmon et al, 2008) and IgA (Muneta et al, 2010), is that it is not influenced by circadian variation. This fact has been demonstrated in dogs (Kanai et al, 2008). It has also been shown that in humans salivary CgA is probably not affected by food intake (Toda et al, 2004).…”

Section: Introductionmentioning

confidence: 65%

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Escribano

Soler

Gutiérrez

et al. 2013

Animal

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The objective of this study was to develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for porcine salivary chromogranin A (CgA) measurements, using a species-specific antibody, and evaluate its behaviour in an acute stress model. Polyclonal antibodies were produced in rabbits immunized with a synthetic porcine fragment of CgA 3592379 and used to develop a sandwich TR-IFMA. This TR-IFMA was analytically validated and showed intra-and inter-assay coefficients of variation of 6.23% and 5.82%, respectively, an analytical limit of detection of 4.27 3 10 23 mg/ml and a limit of quantification of 24.5 3 10 23 mg/ml. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution (r 5 0.975) and recovery tests. When a model of experimental acute stress, in which animals were immobilized for 3 min with a nose snare (stressor stimulus), was applied, a significant increase (P , 0.05) in CgA levels in saliva was detected at 15 min post-stressor stimulus. These results indicate that the assay developed in this study could measure CgA in porcine saliva in a reliable way and that the concentrations of CgA in saliva samples of pigs increase after an acute stress situation.

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“…Nos últimos anos, a utilização de biomarcadores como método de investigação de doenças consolidou-se, como indicadores inovadores de grande potencial para a identificação e o diagnóstico em todo mundo (WANG et al, 2016). (SATO et al, 2002;CRONIN et al, 2003;SARUTA et al, 2005;KANAI et al, 2008;STRIDSBERG et al, 2014 (NOBELS et al, 1997).…”

Section: Os Biomarcadores Salivares E Suas Contribuições Para O Diagnunclassified

Associação da cromogranina A e da amilase salivar com a ansiedade e o estresse de profissionais de enfermagem hospitalar

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(58%) and many of them (48,1%) had mild, moderate or severe anxiety. It was found that in the morning shift the activity of AA changed between the collection times. Already the CgA, presented more changes in the afternoon shift. It was found that the variables age group, number of children, length of work in the institution, weekly workload and medication use were significantly associated with the anxiety of nursing professionals (p<0,05). In addition, the variables: age group, physical activity practice, continuous medication use, length of work in the institution, other employment and smoking, showed an association with workers' stress (p<0,05). Anxiety was found to be associated with stress of nursing professionals (p<0,001). When analyzing the association of anxiety and stress with AA and CgA, statistical significance was found between the group "stress and anxiety" with AA at night shift times (p<0,05). Significance was also found for the "stress and anxiety" group with CgA at night shift, as well as for the "anxiety" group at morning shift (p<0,05). Conclusion: Nursing professionals presented high levels of anxiety and stress, which were associated with changes in CgA and AA at certain times and work shifts, demonstrating that anxiety and stress biomarkers may be possible.

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Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs

Cited by 19 publications

References 16 publications

Catestatin and vasostatin concentrations in healthy dogs

Catestatin and vasostatin concentrations in healthy dogs

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Associação da cromogranina A e da amilase salivar com a ansiedade e o estresse de profissionais de enfermagem hospitalar

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