Circannual Identification and Quantification of Constitutive Heat Shock Proteins (HSP 70) in Goats

Meza-Herrera, César A.; Martı́nez, Laura C.; Aréchiga, C. F.; Bañuelos, Rómulo; Rincón, Ríos; Urrutia, J.; Salinas, H.; Mellado, Miguel

doi:10.1080/09712119.2006.9706560

Cited by 17 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar to our study, HSPs induction was reported in various cell/ tissue types such as leukocytes/lymphocytes [29][30][31], bovine endometrial tissue, bovine conceptuses [32,33], bovine granulosa cells [34] bovine MECs [22], buffalo lymphocytes [35] due to heat stress. It has been reported that heat stress causes an increase in HSPs in virtually all the vertebrates, including mice [36,37] domestic goats [38], humans [39,40], juvenile Hamadryads baboons [41], common carp [42], domestic chickens [43][44][45][46] and domestic turkey [47]. Our results showed an increased accumulation of inducible HSP70 in heat stressed groups at both the protein and mRNA levels, thus supporting the idea that HSP70 can act as a reliable thermal stress biomarker [42,48].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells

Khan

Dou

Wang

et al. 2020

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background: Heat stress is known to affect follicular dynamics, oocyte maturation, and fertilization by impairing steroidogenic ability and viability of bovine granulosa cell (bGCs). The present study explored the physiological and molecular response of bGCs to different heat stress intensities in-vitro. We exposed the primary bGCs to heat stress (HS) at 39°C, 40°C and 41°C along with control samples (38°C) for 2 h. To evaluate the impact of heat stress on bGCs, several in vitro cellular parameters including cell apoptosis, intracellular reactive oxygen species (ROS) accumulation and HSP70 kinetics were assessed by flow cytometry, florescence microscopy and western blot, respectively. Furthermore, the ELISA was performed to confirm the 17β-estradiol (E 2 ) and progesterone (P 4 ) levels. In addition, the RNA sequencing (RNA-Seq) method was used to get the molecular based response of bGCs to different heat treatments. Results: Our findings revealed that the HS significantly decreased the cell viability, E 2 and P 4 levels in bGCs, whereas, increased the cellular apoptosis and ROS. Moreover, the RNA-Seq experiments showed that all the treatments (39°C, 40°C and 41°C) significantly regulated many differentially expressed genes (DEGs) i.e. BCL2L1, STAR, CYP11A1, CASP3, SOD2, HSPA13, and MAPK8IP1 and pathways associated with heat stress, apoptosis, steroidogenesis, and oxidative stress. Conclusively, our data demonstrated that the impact of 40°C treatment was comparatively detrimental for cell viability, apoptosis and ROS accumulation. Notably, a similar trend of gene expression was reported by RT-qPCR for RNA-seq data. Conclusions:Our study presented a worthy strategy for the first time to characterize the cellular and transcriptomic adaptation of bGCs to heat stress (39, 40 and 41°C) in-vitro. The results infer that these genes and pathways reported in present study could be useful candidates/indicators for heat stress research in dairy cattle. Moreover, the established model of bGCs to heat stress in the current study provides an appropriate platform to understand the mechanism of how heat-stressed bGCs can affect the quality of oocytes and developing embryo.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells

Khan

Dou

Wang

et al. 2020

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…Likewise, HSP induction was also reported in various cell/tissue types, such as leukocytes/lymphocytes [67][68][69], bovine endometrial tissue, bovine conceptuses [70,71], bovine MECs [72], and buffalo lymphocytes [73] due to HS. Moreover, it has been reported that HS causes an increase in HSPs in virtually all vertebrates, including mice [74,75], domestic goats [76], humans [77,78], juvenile hamadryas baboons [79], common carp [80], domestic chickens [81][82][83], and domestic turkey [84], thus supporting the idea that HSP70 can act as a reliable thermal stress biomarker. Upregulation of apoptotic genes (BCL2, BAX, CASP3) under HS could lead to a disruption of the potential of mitochondrion transmembrane, resulting in the release of cytochrome c and cellular apoptosis [85].…”

Section: Impact Of Heat Stress On Granulosa Cell Functionmentioning

confidence: 79%

Cellular and Molecular Adaptation of Bovine Granulosa Cells and Oocytes under Heat Stress

Khan

Umer

et al. 2020

Animals

View full text Add to dashboard Cite

Heat stress has long been recognized as a challenging issue that severely influences the reproductive functions of dairy cattle, disrupting oocyte development during fetal growth. These detrimental effects of heat stress are the result of either the hyperthermia associated with heat stress or the physiological adjustments made by the heat-stressed animal to regulate body temperature. In addition, elevated temperatures have been implicated in increasing the production of reactive oxygen species. Thus, understanding the impact of heat stress on reproductive functions, from a cellular to molecular level, might help in selecting heat-resilient dairy cattle and developing heat stress mitigation strategies. In the present paper, we have attempted to describe the changes in the reproductive system and function of dairy cattle in response to heat stress by reviewing the latest literature in this area. The review provides useful knowledge on the cellular and genetic basis of oocyte and granulosa cells in heat-stressed dairy cattle, which could be helpful for future research in this area.

show abstract

“…Some studies show that cells in better heat-adapted bovine breeds produce lower HSP70 concentrations than in less adapted breeds (10). Similarly, in goats adapted to the desert climate, HSP70 levels were higher than in less adapted goats (14).…”

Section: Resultsmentioning

confidence: 99%

“…HSP70 synthesis increases when the cell is exposed to high temperatures, preventing damage to proteins from occurring (3). This response comprises part of the thermal acclimation mechanism of cells to high temperatures and for this reason it may be used as an indicator for the adaptation capacity of animals to heat stress (14).…”

mentioning

confidence: 99%

Effects of the season on physiological and endocrine traits and on HSP70 in Saanen goats under Mediterranean climate conditions

Yılmaz

Altin

Kiral

et al. 2018

Medycyna Weterynaryjna

View full text Add to dashboard Cite

This study was conducted to determine the impact of heat stress on some physiological and endocrine traits in Saanen goats raised under Mediterranean climate conditions. The effects of thermal stress on heart rate (HR), respiration rate (RR) and rectal temperature (RT) on plasma total trii-odothyronine (T3), thyroxine (T4), Cortisol (C), and HSP70 concentrations were evaluated on twenty two Saanen goats of different ages in the second week of April 2013, July 2013, October 2013 and January 2014. Climatic data such as temperature (°C) and relative humidity (%) were recorded from Spring 2013 to Winter 2014. Live body and BCS values were also recorded during this period. The physiological parameters above were measured twice on each experiment day (morning and afternoon) in all seasons. Blood samples were collected in each afternoon of the experiment day to analyze T3, T4 C, and HSP70. All data were analyzed. According to the values of rectal temperature (RT), it was estimated that the goats were under extreme heat stress only in the summer season. The heart rate (HR) values in the winter season for morning and noon periods were found statistically significant (p < 0.05). The average respiratory rate (RR) in the spring season was found significantly lower. On the other hand, the RR for the noon period in the summer was higher than in the fall and winter seasons (p < 0.05). There was a significant difference (p < 0.05) between summer and fall seasons for C values. The highest value (96.62 ng/ml) was obtained in spring, whereas the lowest (60.58 ng/ml) in the fall. T3 levels in the fall and winter were found to be statistically significant (p < 0.05). They were the highest in the winter and spring, and the lowest in fall. T4 and T4/T3 levels in the winter were found to be statistically higher than in other seasons (p < 0.05). The lowest value for T4 was found in the fall and for T4/T3 in summer. Mean HSP70 value in spring was found to be statistically low (p < 0.05). The changes in THI values in different seasons, particularly between mornings and afternoons, indicated that thermal stress was evident, and that the animals became resistant to it eventually. The fluctuations of the C, T3, T4 and HSP70 values were indicators of the animals’ reaction to thermal stress. The THI values in spring, which were between 16 and 18 THI, may be considered within the ideal comfort zone for goats. It was observed that Saanen goats were able to adapt to seasonal weather changes in the environmental conditions of the region....

show abstract

Circannual Identification and Quantification of Constitutive Heat Shock Proteins (HSP 70) in Goats

Cited by 17 publications

References 13 publications

Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells

Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells

Cellular and Molecular Adaptation of Bovine Granulosa Cells and Oocytes under Heat Stress

Effects of the season on physiological and endocrine traits and on HSP70 in Saanen goats under Mediterranean climate conditions

Contact Info

Product

Resources

About