Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach

Zaldibar, Beñat; Cancio, Ibon; Marigómez, Ionan

doi:10.1007/s00441-004-0960-0

Cited by 42 publications

(25 citation statements)

References 45 publications

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“…This temporal pattern may be analogous to the discovery in yeast that metabolic and cell division events are compartmentalized in time (Tu et al, 2005). Rhythmic patterns of cell proliferation have been reported in M. galloprovincialis, and revealed that epithelial cell turnover in the digestive gland (a tissue that is constantly undergoing renewal) were synchronized with the tidal cycle, increasing during low tide and decreasing during high tide (Zaldibar et al, 2004). It has been suggested that the functional rationale behind the temporal compartmentalization of physiological processes is to ensure that processes that might be deemed sensitive to oxidative stress are temporally separated from periods when reactive oxygen species may be present.…”

Section: Orchestration Of Life At Intertidal Extremessupporting

confidence: 72%

The environmentally tuned transcriptomes ofMytilusmussels

Lockwood

Connor

Gracey

2015

Journal of Experimental Biology

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Transcriptomics is a powerful tool for elucidating the molecular mechanisms that underlie the ability of organisms to survive and thrive in dynamic and changing environments. Here, we review the major contributions in this field, and we focus on studies of mussels in the genus Mytilus, which are well-established models for the study of ecological physiology in fluctuating environments. Our review is organized into four main sections. First, we illustrate how the abiotic forces of the intertidal environment drive the rhythmic coupling of gene expression to diel and tidal cycles in Mytilus californianus. Second, we discuss the challenges and pitfalls of conducting transcriptomic studies in field-acclimatized animals. Third, we examine the link between transcriptomic responses to environmental stress and biogeographic distributions in blue mussels, Mytilus trossulus and Mytilus galloprovincialis. Fourth, we present a comparison of transcriptomic datasets and identify 175 genes that share common responses to heat stress across Mytilus species. Taken together, these studies demonstrate that transcriptomics can provide an informative snapshot of the physiological state of an organism within an environmental context. In a comparative framework, transcriptomics can reveal how natural selection has shaped patterns of transcriptional regulation that may ultimately influence biogeography.

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Section: Orchestration Of Life At Intertidal Extremessupporting

confidence: 72%

The environmentally tuned transcriptomes ofMytilusmussels

Lockwood

Connor

Gracey

2015

Journal of Experimental Biology

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“…A number of intertidal organisms, particularly crustaceans, display entrained behavioral rhythms that follow persistent circadian (28) and tidal patterns (29,30) even when held under constant conditions. Similarly, mussels are reported to show entrained tidal patterns of cell division (31). These findings have led to questions regarding the existence of a tidal clock and the nature of its regulation, with some data suggesting the presence of a circatidal clock with a period of 12.4 h (28), and other data supporting an alternative hypothesis that postulates that two circalunidian clocks with 24.8-h periods running in antiphase to one another could generate peaks in activity every 12.4 h (32).…”

Section: Discussionmentioning

confidence: 95%

Circadian cycles are the dominant transcriptional rhythm in the intertidal musselMytilus californianus

Connor

Gracey

2011

Proc. Natl. Acad. Sci. U.S.A.

120

135

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Residents in the marine intertidal, the zone where terrestrial and marine habitats converge, inhabit an environment that is subject to both the 24-h day and night daily rhythm of the terrestrial earth and also the 12.4-h ebb and flow of the tidal cycle. Here, we investigate the relative contribution of the daily and tidal cycle on the physiology of intertidal mussels, Mytilus californianus, by monitoring rhythms of gene expression in both simulated and natural tidal environments. We report that >40% of the transcriptome exhibits rhythmic gene expression, and that depending on the specific tidal conditions, between 80% and 90% of the rhythmic transcripts follow a circadian expression pattern with a period of 24 to 26 h. Consistent with the dominant effect of the circadian cycle we show that the expression of clock genes oscillates with a 24-h period. Our data indicate that the circadian 24-h cycle is the dominant driver of rhythmic gene expression in this intertidal inhabitant despite the profound environmental and physiological changes associated with aerial exposure during tidal emergence.

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“…The 5-bromodeoxyuridine (BrdU)-labeling method is useful for studying cell cycle kinetics and DNA replication and thus is a widely used marker for cell proliferation (Zaldibar et al 2004;Salic and Mitchison 2008). A stock solution of 10 mM BrdU (MP Biomedicals) was prepared in L-15 M medium and stored at -20°C.…”

Section: Brdu Testmentioning

confidence: 99%

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

2010

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Molluscan in vitro technology allows the study of the differentiation of isolated cells undergoing experimental manipulations. We have used the immunofluorescence technique and laser scanning microscopy to investigate the organization of muscle proteins (actin, myosin, paramyosin, and twitchin) and the localization of neurotransmitters (serotonin and FMRFamide) in cultured mussel larval cells. Differentiation into muscle and neuron-like cells occurs during the cultivation of mussel cells from premyogenic and prenervous larval stages. Muscle proteins are colocalized in contractile cells through all stages of cultivation. The cultivation of mussel cells on various substrates and the application of integrin receptor blockers suggest that an integrin-dependent mechanism is involved in cell adhesion and differentiation. Dissociated mussel cells aggregate and become self-organized in culture. After 20 days of cultivation, they form colonies in which serotonin- and FMRFamide-immunoreactive cells are located centrally, whereas muscle cells form a contractile network at the periphery. The pattern of thick and thin filaments in cultivated mussel cells changes according to the scenario of muscle arrangement in vivo: initially, a striated pattern of muscle filaments forms but is then replaced by a smooth muscle pattern with a diffuse distribution of muscle proteins, typical of muscles of adult molluscs. Myogenesis in molluscs thus seems to be a highly dynamic and potentially variable process. Such a "flexible" developmental program can be regarded as a prerequisite for the evolution of the wide variety of striated and smooth muscles in larval and adult molluscs.

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Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach

Cited by 42 publications

References 45 publications

The environmentally tuned transcriptomes ofMytilusmussels

The environmentally tuned transcriptomes ofMytilusmussels

Circadian cycles are the dominant transcriptional rhythm in the intertidal musselMytilus californianus

Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)

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