circEXOC5 promotes acute lung injury through the PTBP1/Skp2/Runx2 axis to activate autophagy

Gao, Pei; Wu, Beirong; Ding, Ying; Yin, Bingru; Gu, Haoxiang

doi:10.26508/lsa.202201468

Cited by 8 publications

(4 citation statements)

References 56 publications

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“…It is required for photoreceptor ciliogenesis and retinal development [ 40 ]. A recent work demonstrated that circEXOC5 (hsa_circ_0004399) is one of the mostly significantly up regulated circRNAs identified by microarray in lung macrophages from acute lung injured mice compared to control mice finally demonstrating a pivotal role of this circRNAs in promoting acute lung injury in mice [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Platelet-derived circRNAs signature in patients with gastroenteropancreatic neuroendocrine tumors

et al. 2023

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Background Neuroendocrine tumors (NETs) early diagnosis is a clinical challenge that require a deep understanding of molecular and genetic features of this heterogeneous group of neoplasms. However, few biomarkers exist to aid diagnosis and to predict prognosis and treatment response. In the oncological field, tumor-educated platelets (TEPs) have been implicated as central players in the systemic and local responses to tumor growth, thereby altering tumor specific RNA profile. Although TEPs have been found to be enriched in RNAs, few studies have investigated the potential of a type of RNA, circular RNAs (circRNA), as platelet-derived biomarkers for cancer. In this proof-of-concept study, we aim to demonstrate whether the circRNAs signature of tumor educated platelets can be used as a liquid biopsy biomarker for the detection of gastroenteropancreatic (GEP)-NETs and the prediction of the early response to treatment. Methods We performed a 24-months, prospective proof-of-concept study in men and women with histologically proven well-differentiated G1-G2 GEP-NET, aged 18–80 years, naïve to treatment. We performed a RNAseq analysis of circRNAs obtained from TEPs samples of 10 GEP-NETs patients at baseline and after 3 months from therapy (somatostatin analogs or surgery) and from 5 patients affected by non-malignant endocrinological diseases enrolled as a control group. Results Statistical analysis based on p < 0.05 resulted in the identification of 252 circRNAs differentially expressed between GEP-NET and controls of which 109 were up-regulated and 143 were down-regulated in NET patients. Further analysis based on an FDR value ≤ 0.05 resulted in the selection of 5 circRNAs all highly significant downregulated. The same analysis on GEP-NETs at baseline and after therapy in 5 patients revealed an average of 4983 remarkably differentially expressed circRNAs between follow-up and baseline samples of which 2648 up-regulated and 2334 down-regulated, respectively. Applying p ≤ 0.05 and FDR ≤ 0.05 filters, only 3/5 comparisons gave statistically significant results. Conclusions Our findings identified for the first time a circRNAs signature from TEPs as potential diagnostic and predictive biomarkers for GEP-NETs.

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Section: Discussionmentioning

confidence: 99%

Platelet-derived circRNAs signature in patients with gastroenteropancreatic neuroendocrine tumors

et al. 2023

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“…We cut the PVDF membrane while retaining sufficient width and length to allow different positions of the PVDF membrane to bond with different primary antibodies. Then blotted with primary antibodies for LC3 (1:1000, ab192890, Abcam) 54 , P62 (1:1000, ab109012, Abcam) 55 , SIRT3 (1:500, #5490, CST) 56 , SOD2 (1:1000, #13141, CST) 57 , acetylated SOD2 (1:1000, ab137037, Abcam) 58 and GAPDH (1:2000, 60004-1-Ig, Proteintech) 59 overnight at 4 °C. Then, incubate with the secondary antibody for 1.5 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Curcumin regulates autophagy through SIRT3-SOD2-ROS signaling pathway to improve quadriceps femoris muscle atrophy in KOA rat model

Ye,

Long,

Yang

et al. 2024

Sci Rep

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Knee osteoarthritis (KOA) usually leads to quadriceps femoris atrophy, which in turn can further aggravate the progression of KOA. Curcumin (CUR) has anti-inflammatory and antioxidant effects and has been shown to be a protective agent for skeletal muscle. CUR has been shown to have a protective effect on skeletal muscle. However, there are no studies related to whether CUR improves KOA-induced quadriceps femoris muscle atrophy. We established a model of KOA in rats. Rats in the experimental group were fed CUR for 5 weeks. Changes in autophagy levels, reactive oxygen species (ROS) levels, and changes in the expression of the Sirutin3 (SIRT3)-superoxide dismutase 2 (SOD2) pathway were detected in the quadriceps femoris muscle of rats. KOA led to quadriceps femoris muscle atrophy, in which autophagy was induced and ROS levels were increased. CUR increased SIRT3 expression, decreased SOD2 acetylation and ROS levels, inhibited the over-activation of autophagy, thereby alleviating quadriceps femoris muscle atrophy and improving KOA. CUR has a protective effect against quadriceps femoris muscle atrophy, and KOA is alleviated after improvement of quadriceps femoris muscle atrophy, with the possible mechanism being the reduction of ROS-induced autophagy via the SIRT3-SOD2 pathway.

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“…So far, there have been a few studies focusing on the detection of either ALP or RUNX2 with the help of graphene-based biosensors. Mahato et al [34] developed an impedimetric biosensor using gold-nano-dendroids and graphene oxide (GO) detecting ALP in human serum samples, Liu et al [35] describe a fluorescent assay based on GO and λ exonuclease for ALP activity, while to the best of our knowledge, RUNX2 detection is still performed using classical techniques like quantitative PCR (qPCR) [36], Western blot [37], and immunohistochemistry [38], while no progress has been made regarding the development of a novel electrochemical biosensor to detect this biomarker. Ma'arif et al [22] compared two techniques used for RUNX2 detection in bone tissue, i.e., Western blot and immunohistochemistry, investigating 70 articles, and concluded that the Western blot method is superior in terms of sensitivity, selectivity, cost, and detection time.…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach Using Reduced Graphene Oxide for the Detection of ALP and RUNX2 Osteogenic Biomarkers

Chiticaru,

Ioniță

2024

CIMB

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In this work, we propose a new technique involving the modification of commercial screen-printed carbon electrodes with electrochemically reduced graphene oxide to serve as the starting point of a future electrochemical biosensor for the detection of two osteogenic biomarkers: alkaline phosphatase (ALP) and Runt-related transcription factor 2 (RUNX2). The electrodes were characterized after each modification by cyclic voltammetry and electrochemical impedance spectroscopy, showing the appropriate electrochemical characteristics for each modification type. The results obtained from scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and contact angle measurements are well correlated with each other, demonstrating the successful modification of the electrodes with graphene oxide and its subsequent reduction. The bioreceptors were immobilized on the electrodes by physical adsorption, which was confirmed by electrochemical methods, structural characterization, and contact angle measurements. Finally, the functionalized electrodes were incubated with the specific target analytes and the detection relied on monitoring the electrochemical changes occurring after the hybridization process. Our results indicated that the pilot platform has the ability to detect the two biomarkers up to 1 nM, with increased sensitivity observed for RUNX2, suggesting that after further optimizations, it has a high potential to be employed as a future biosensor.

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circEXOC5 promotes acute lung injury through the PTBP1/Skp2/Runx2 axis to activate autophagy

Cited by 8 publications

References 56 publications

Platelet-derived circRNAs signature in patients with gastroenteropancreatic neuroendocrine tumors

Platelet-derived circRNAs signature in patients with gastroenteropancreatic neuroendocrine tumors

Curcumin regulates autophagy through SIRT3-SOD2-ROS signaling pathway to improve quadriceps femoris muscle atrophy in KOA rat model

A Novel Approach Using Reduced Graphene Oxide for the Detection of ALP and RUNX2 Osteogenic Biomarkers

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