circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Yang, Xiaojiao; Sun, Quan; Song, Yongming; Li, Wenli

doi:10.1155/2021/3997045

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…This raises the possibility of a mechanism by which circHUWE1 functions to enhance the activity of HUWE1 protein. This is consistent with the conflicting literature suggesting different functional roles for circHUWE1 [29][30][31][32] . HUWE1 is an E3 ubiquitin ligase that regulates different proteins in a context specific manner.…”

Section: Core Essential Circrnassupporting

confidence: 92%

Systematic loss of function screens identify pathway specific functional circular RNAs

Liu

Neve

Perlaza-Jiménez

et al. 2022

Preprint

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Circular RNAs (circRNAs) are covalently closed single stranded RNAs that are produced by RNA back-splicing. A small number of circRNAs have been implicated as functional, however, we still lack systematic understanding of cellular processes and signalling pathways that are regulated by circRNAs. A major gap, in understanding circRNAs functions is the ability to define pathways that are regulated by circRNAs. Here, we generated a pooled shRNA library targeting the back-splice junction of 3,354 human circRNAs that are expressed at low to high levels in humans. We used this library for loss of function proliferation screens in a panel of 18 cancer cell lines from four tissue types that harbour mutations leading to constitutive activity of defined pathways. Using this dataset, we identify context specific and non-specific circRNAs. We validated these observations with a secondary screen and uncovered a role forcircRERE, a cell essential circRNA that regulates ferroptosis. Furthermore, we characterised the functional roles of pathway-specific circRNA,circSMAD2, a novel regulator of the WNT pathway andcircMTO1, a regulator of MAPK signalling in aPTENdependent manner. Our work sheds light on molecular pathways regulated by circRNAs and provides a catalogue of circRNAs with a measurable function in human cells.

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Section: Core Essential Circrnassupporting

confidence: 92%

Systematic loss of function screens identify pathway specific functional circular RNAs

Liu

Neve

Perlaza-Jiménez

et al. 2022

Preprint

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“…Since the aforementioned study used different cells from the ones used in the present study, it is hypothesized that the different results may be caused by cell differences. In addition to ALDOA, other targets of miR-34a-5p, including TNFAIP8, flotillin-2, and hexokinase-1 have been revealed ( 18 , 40 , 41 ). Among them, Li et al reported that hexokinase-1 is also the key enzyme of glycolysis and participates in CRC progression ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

Wang

Yan

et al. 2023

Oncol Rep

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Colorectal cancer (CRC) is an aggressive tumor, whose development is considered to be modulated by certain long non-coding RNAs (lncRNAs). Therefore, the aim of the present study was to investigate the regulatory mechanism of lncRNA NONHSAG028908.3 on CRC. Data from The Cancer Genome Atlas (TCGA) database revealed that NONHSAG028908.3 was increased in CRC tissues compared with normal tissues (P<0.001). The results of reverse transcription-quantitative PCR indicated that NONHSAG028908.3 was upregulated in four types of CRC cells compared with that in NCM460, a normal colorectal cell line. MTT, BrdU, and flow cytometric assays were applied to evaluate CRC cell growth. The migratory and invasive abilities of CRC cells were detected using wound healing and Transwell assays. Silencing of NONHSAG028908.3 inhibited proliferation, migration, and invasion of CRC cells. A dual-luciferase reporter assay demonstrated that NONHSAG028908.3 served as a sponge to combine with microRNA (miR)-34a-5p. MiR-34a-5p suppressed the aggressiveness of CRC cells. The effects induced by NONHSAG028908.3 knockdown were partly reversed by inhibition of miR-34a-5p. Furthermore, miR-34a-5p, a target of NONHSAG028908.3, modulated aldolase, fructose-bisphosphate A (ALDOA) expression in a negative feedback manner. Suppression of NONHSAG028908.3 notably decreased ALDOA expression, which was rescued via silencing of miR-34a-5p. Moreover, suppression of ALDOA revealed the inhibitory action on CRC cell growth and migration. In summary, the data of the present study indicate that NONHSAG028908.3 may positively regulate ALDOA via sponging miR-34a-5p, thereby promoting malignant activities in CRC.

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“…Notable, many circRNAs could serve as a sponge of miR-34a-5p, including circOgdh (also named mmu_circ_0000231) 176 , circMED12L 180 , circ_FURIN 181 , circ_CSNK1E 182 , circ0036602 183 , circ-LRP1B 184 , circHUWE1 185 , circITGA7 186 , circNFIX 187 , 188 , circRNA-CIDN 189 , circ_0009910 190 , circ_0039569 191 , hsa_circ_0018069 192 , suggesting that these circRNAs may promote lipid synthesis but suppress lipid accumulation by regulating miR-34a-5p/ACSL, ATGL, and ADAM10 axis.…”

Section: The Potential Role and Mechanism Of The Circrna/mirna Axis I...mentioning

confidence: 99%

The potential role and mechanism of circRNA/miRNA axis in cholesterol synthesis

Chen¹,

Xu²,

Wu³

et al. 2023

Int. J. Biol. Sci.

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Cholesterol levels are an initiating risk factor for atherosclerosis. Many genes play a central role in cholesterol synthesis, including HMGCR, SQLE, HMGCS1, FDFT1, LSS, MVK, PMK, MVD, FDPS, CYP51, TM7SF2, LBR, MSMO1, NSDHL, HSD17B7, DHCR24, EBP, SC5D, DHCR7, IDI1/2. Especially, HMGCR, SQLE, FDFT1, LSS, FDPS, CYP51, and EBP are promising therapeutic targets for drug development due to many drugs have been approved and entered into clinical research by targeting these genes. However, new targets and drugs still need to be discovered. Interestingly, many small nucleic acid drugs and vaccines were approved for the market, including Inclisiran, Patisiran, Inotersen, Givosiran, Lumasiran, Nusinersen, Volanesorsen, Eteplirsen, Golodirsen, Viltolarsen, Casimersen, Elasomeran, Tozinameran. However, these agents are all linear RNA agents. Circular RNAs (circRNAs) may have longer half-lives, higher stability, lower immunogenicity, lower production costs, and higher delivery efficiency than these agents due to their covalently closed structures. CircRNA agents are developed by several companies, including Orna Therapeutics, Laronde, and CirCode, Therorna. Many studies have shown that circRNAs regulate cholesterol synthesis by regulating HMGCR, SQLE, HMGCS1, ACS, YWHAG, PTEN, DHCR24, SREBP-2, and PMK expression. MiRNAs are essential for circRNA-mediated cholesterol biosynthesis. Notable, the phase II trial for inhibiting miR-122 with nucleic acid drugs has been completed. Suppressing HMGCR, SQLE, and miR-122 with circRNA_ABCA1, circ-PRKCH, circEZH2, circRNA-SCAP, and circFOXO3 are the promising therapeutic target for drug development, specifically the circFOXO3. This review focuses on the role and mechanism of the circRNA/miRNA axis in cholesterol synthesis in the hope of providing knowledge to identify new targets.

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circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Cited by 7 publications

References 42 publications

Systematic loss of function screens identify pathway specific functional circular RNAs

Systematic loss of function screens identify pathway specific functional circular RNAs

NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

The potential role and mechanism of circRNA/miRNA axis in cholesterol synthesis

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