circINSR Promotes Proliferation and Reduces Apoptosis of Embryonic Myoblasts by Sponging miR-34a

Shen, Xuemei; Zhang, Xiaoyan; Ru, Wenxiu; Huang, Yongzhen; Lan, Xianyong; Lei, Chuzhao; Chen, Hong

doi:10.1016/j.omtn.2019.12.032

Cited by 34 publications

(33 citation statements)

References 46 publications

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“…The circINSR is highly homologous to human has_circ_0048966, formed by the head-to-tail splicing of INSR second exon (552 bp), and is mainly expressed in the cytoplasm. In previous research, circINSR regulates cells proliferation and apoptosis through miR-34a-modulated Bcl-2 and CyclinE2 expression (17). In this study, we found that circINSR could also sponge the miR-15/16 family.…”

Section: Discussionsupporting

confidence: 56%

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CircINSR regulates fetal bovine muscle and fat development

Shen

Tang

et al. 2020

Preprint

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Background: The level of muscle development directly affects the production efficiency of livestock, and the content of intramuscular fat (IMF) is an important factor affecting meat quality. Nevertheless, the molecular mechanism of embryonic circular RNA in muscle and IMF development remains largely unknown. Methods: In this study, we isolated myoblasts and intramuscular preadipocytes from fetal bovine skeletal muscle. Oil Red O and BODIPY staining were used to identify lipid droplets of preadipocytes, and anti-MyHC immunofluorescence was used to identify myotubes differentiated from myoblasts. Bioinformatics, dual fluorescence reporter system, and RNA immunoprecipitation were used to determine the interactions between circINSR and miR-15/16 family. Molecular and biochemical assays were used to confirm the role of circINSR in myoblasts and intramuscular preadipocytes. Results: We found that the isolated myoblasts and preadipocytes can differentiate normally. Besides, circINSR severed as a sponge of miR-15/16 family, which targeting CyclinD1 and Bcl-2. CircINSR overexpression significantly promoted myoblasts and preadipocytes proliferation, and inhibited cell apoptosis. In addition, circINSR inhibited preadipocytes adipogenesis by alleviating the inhibition of miR-15/16 on target genes FOXO1 and EPT1. Conclusions: Taken together, our study demonstrated circINSR as a regulator of embryonic muscle and IMF development.

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Section: Discussionsupporting

confidence: 56%

“…In existing reports, circFUT10 and circFGFR4 regulate the muscle development related genes through sponge miR-133a and miR-107 (15,16). CircINSR adsorb miR-34a to promote the proliferation of bovine myoblasts (17). CircTshz2-1 and circArhgap5-2 have been shown to be indispensable regulators of fat formation (18).…”

Section: Introductionmentioning

confidence: 99%

CircINSR regulates fetal bovine muscle and fat development

Shen

Tang

et al. 2020

Preprint

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“…In the later stage of infection, the virus promotes cell apoptosis to facilitate release and spread. MiR-34a, miR-222, miR-98, and miR-21 play an important role in cell apoptosis (Zhou et al, 2017 ; Tong et al, 2019 ; Zheng et al, 2019 ; Shen et al, 2020 ). Therefore, many researchers focus on the role of miRNAs in virus-induced cell apoptosis.…”

Section: The Role Of Mirnas In Cvb3-induced Vmcmentioning

confidence: 99%

The Role of Non-coding RNAs in Viral Myocarditis

Zhang

Xiong

Zeng

et al. 2020

Front. Cell. Infect. Microbiol.

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Viral myocarditis (VMC) is a disease characterized as myocardial parenchyma or interstitium inflammation caused by virus infection, especially Coxsackievirus B3 (CVB3) infection, which has no accurate non-invasive examination for diagnosis and specific drugs for treatment. The mechanism of CVB3-induced VMC may be related to direct myocardial damage of virus infection and extensive damage of abnormal immune response after infection. Non-coding RNA (ncRNA) refers to RNA that is not translated into protein and plays a vital role in many biological processes. There is expanding evidence to reveal that ncRNAs regulate the occurrence and development of VMC, which may provide new treatment or diagnosis targets. In this review, we mainly demonstrate an overview of the potential role of ncRNAs in the pathogenesis, diagnosis and treatment of CVB3-induced VMC.

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“…CircFUT10 and circFGFR4 have been reported to regulate the muscle development-related genes through the sponging of miR-133a and miR-107 (Li et al, 2018a , b ). CircINSR has been shown to adsorb miR-34a to promote the proliferation of bovine myoblasts (Shen et al, 2020 ). CircTshz2-1 and circArhgap5-2 have been shown to be indispensable regulators of fat formation (Arcinas et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…Based on our previous studies, we have focused on a circRNA derived from the insulin receptor gene (INSR) in this study. CircINSR is named after the INSR gene and is formed by the cyclization of the second exon of INSR (Shen et al, 2020 ). A previous study indicated that circINSR could affect the proliferation and apoptosis of bovine embryonic myoblasts by adsorbing miR-34a; however, whether circINSR interacts with other miRNAs and whether circINSR plays a role in preadipocyte development remains unclear.…”

Section: Introductionmentioning

confidence: 99%

CircINSR Regulates Fetal Bovine Muscle and Fat Development

Shen

Tang

et al. 2021

Front. Cell Dev. Biol.

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The level of muscle development in livestock directly affects the production efficiency of livestock, and the contents of intramuscular fat (IMF) is an important factor that affects meat quality. However, the molecular mechanisms through which circular RNA (circRNA) affects muscle and IMF development remains largely unknown. In this study, we isolated myoblasts and intramuscular preadipocytes from fetal bovine skeletal muscle. Oil Red O and BODIPY staining were used to identify lipid droplets in preadipocytes, and anti-myosin heavy chain (MyHC) immunofluorescence was used to identify myotubes differentiated from myoblasts. Bioinformatics, a dual-fluorescence reporter system, RNA pull-down, and RNA-binding protein immunoprecipitation were used to determine the interactions between circINSR and the micro RNA (miR)-15/16 family. Molecular and biochemical assays were used to confirm the roles played by circINSR in myoblasts and intramuscular preadipocytes. We found that isolated myoblasts and preadipocytes were able to differentiate normally. CircINSR was found to serve as a sponge for the miR-15/16 family, which targets CCND1 and Bcl-2. CircINSR overexpression significantly promoted myoblast and preadipocyte proliferation and inhibited cell apoptosis. In addition, circINSR inhibited preadipocyte adipogenesis by alleviating the inhibition of miR-15/16 against the target genes FOXO1 and EPT1. Taken together, our study demonstrated that circINSR serves as a regulator of embryonic muscle and IMF development.

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circINSR Promotes Proliferation and Reduces Apoptosis of Embryonic Myoblasts by Sponging miR-34a

Cited by 34 publications

References 46 publications

CircINSR regulates fetal bovine muscle and fat development

CircINSR regulates fetal bovine muscle and fat development

The Role of Non-coding RNAs in Viral Myocarditis

CircINSR Regulates Fetal Bovine Muscle and Fat Development

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