CircNf1-mediated CXCL12 expression in the spinal cord contributes to morphine analgesic tolerance

Xiu-juan, Bai; Huang, Yongtian; Zhang, Kun; Huang, Wan; Mu, Yanyu; Li, Yujuan; Ouyang, Handong

doi:10.1016/j.bbi.2022.09.018

Cited by 15 publications

(8 citation statements)

References 53 publications

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“…The rSNP annotation data were collected from the rSNPBase 3.1 database. 29,30 There are seven types of regulatory elements, including 7,562,592 annotation terms for transcription factor binding regions (TFBRs) from the Encyclopaedia of DNA Elements (ENCODE) project, 31 212,837 for chromatin interactive regions (CIRs) from the ENCODE project, 31 2,794 for mature microRNA (miRNA) regions from miRbase, 32 384,284 for predicted miRNA target sites from TargetScan 33 and miRNAda, [34][35][36][37] 211,749 for long non-coding RNA (lncRNA) regions from LNCipedia, 38 38,916 for topologically associated domains (TADs) from ENCODE-processed data, and 312,673 for circRNAs from CircNet. 39 Except for circRNAs, target gene analyses were performed on the other six types of regulatory elements to get corresponding elements-gene (E-G) pairs.…”

Section: Methodsmentioning

confidence: 99%

Integrating genome-wide association study with regulatory SNP annotations identified novel candidate genes for osteoporosis

Jia

et al. 2023

Bone Joint Res

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AimsOsteoporosis (OP) is a metabolic bone disease, characterized by a decrease in bone mineral density (BMD). However, the research of regulatory variants has been limited for BMD. In this study, we aimed to explore novel regulatory genetic variants associated with BMD.MethodsWe conducted an integrative analysis of BMD genome-wide association study (GWAS) and regulatory single nucleotide polymorphism (rSNP) annotation information. Firstly, the discovery GWAS dataset and replication GWAS dataset were integrated with rSNP annotation database to obtain BMD associated SNP regulatory elements and SNP regulatory element-target gene (E-G) pairs, respectively. Then, the common genes were further subjected to HumanNet v2 to explore the biological effects.ResultsThrough discovery and replication integrative analysis for BMD GWAS and rSNP annotation database, we identified 36 common BMD-associated genes for BMD irrespective of regulatory elements, such as FAM3C (pdiscovery GWAS = 1.21 × 10-25, preplication GWAS = 1.80 × 10-12), CCDC170 (pdiscovery GWAS = 1.23 × 10-11, preplication GWAS = 3.22 × 10-9), and SOX6 (pdiscovery GWAS = 4.41 × 10-15, preplication GWAS = 6.57 × 10-14). Then, for the 36 common target genes, multiple gene ontology (GO) terms were detected for BMD such as positive regulation of cartilage development (p = 9.27 × 10-3) and positive regulation of chondrocyte differentiation (p = 9.27 × 10-3).ConclusionWe explored the potential roles of rSNP in the genetic mechanisms of BMD and identified multiple candidate genes. Our study results support the implication of regulatory genetic variants in the development of OP.Cite this article: Bone Joint Res 2023;12(2):147–154.

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Section: Methodsmentioning

confidence: 99%

Integrating genome-wide association study with regulatory SNP annotations identified novel candidate genes for osteoporosis

Jia

et al. 2023

Bone Joint Res

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“…Lastly, recent findings revealed the involvement of circRNA to cytokine pathway in order to mediate morphine analgesic tolerance [185]. In particular, CircNf1 resulted in being highly expressed in the dorsal horns of morphine-treated rats and, acting as a sponge for miRNA665 and miRNA330-3p, enhances the expression of the CXC motif chemokine ligand 12 (CXCL12), a homeostatic chemokine essential not only in the modulation of morphine analgesic effects in chronic pain but also in neurodevelopmental process, maturation of the CNS, neuronal toxicity and transmission, neuroglial interactions and nerve regeneration [186,187].…”

Section: Upcoming Epigenetic Effectors In Nociceptionmentioning

confidence: 99%

“…Studies on epigenetics and pain remain largely preclinical and investigate the theoretical ability of epigenetics to alter the nociceptive pathways both in the periphery and centrally [21]. Mechanisms, actors and targets have been recently reviewed [22] and recently lncRNAs and circRNAs have been included in the list of the upcoming epigenetic modulators in nociception [184,185].…”

Section: Upcoming Epigenetic Effectors In Nociceptionmentioning

confidence: 99%

Molecular and Epigenetic Aspects of Opioid Receptors in Drug Addiction and Pain Management in Sport

Mazzeo

Meccariello

Guatteo

2023

IJMS

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Opioids are substances derived from opium (natural opioids). In its raw state, opium is a gummy latex extracted from Papaver somniferum. The use of opioids and their negative health consequences among people who use drugs have been studied. Today, opioids are still the most commonly used and effective analgesic treatments for severe pain, but their use and abuse causes detrimental side effects for health, including addiction, thus impacting the user’s quality of life and causing overdose. The mesocorticolimbic dopaminergic circuitry represents the brain circuit mediating both natural rewards and the rewarding aspects of nearly all drugs of abuse, including opioids. Hence, understanding how opioids affect the function of dopaminergic circuitry may be useful for better knowledge of the process and to develop effective therapeutic strategies in addiction. The aim of this review was to summarize the main features of opioids and opioid receptors and focus on the molecular and upcoming epigenetic mechanisms leading to opioid addiction. Since synthetic opioids can be effective for pain management, their ability to induce addiction in athletes, with the risk of incurring doping, is also discussed.

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“…The interaction between mRNA and miRNA was analyzed using the miRNA-mRNA professional prediction websites TargetScan (http://www.targetscan.org/) and miRanda. [6][7][8] The 2 sets of lncRNA-miRNA and miR-NA-mRNA data were intersected again to obtain miRNAs associated with both the target lncRNAs and mRNAs. Based on these key RNAs, an lncRNA-miRNA-mRNA ceRNA network of PTC was established.…”

Section: Predict Microrna and Build Cerna Networkmentioning

confidence: 99%

Significance of LINC02082 and LOC105369812 in differentiating papillary thyroid cancer from benign nodules

Xu,

Liu,

et al. 2023

Medicine

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Objective: To explore the significance of LINC02082 and LOC105369812 in the differential diagnosis of papillary thyroid carcinoma (PTC) and benign nodules. Methods: Cancer tissues and benign nodules from 8 patients were sequenced and constructed using high-throughput sequencing. Differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) with significant differences were screened. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed on the mRNAs co-expressed by DEmRNAs and DElncRNAs. LncRNAs with significant differences, good consistency, and enrichment in the PI3K-AKt signaling pathway were selected as candidate lncRNAs, and the target lncRNAs were screened by correlation analysis. Target lncRNAs and co-expressed mRNAs enriched in the PI3K-AKt signaling pathway and microRNAs (miRNAs) interacting with each other were used to construct a competing endogenous RNA (ceRNA) network. Finally, the PTC-related gene set (GSE33630) was downloaded from the GEO database and the expression of the genes obtained by sequencing was compared. Differential expression was verified using quantitative real-time PCR (qRT-PCR). Finally, the receiver operating characteristic (ROC) curve was used to evaluate the value of the target lncRNAs in diagnosis, when used alone or in combination. Results: A total of 1113 differential RNAs (DE RNAs) were identified, of which 338 were DElncRNAs and 775 were DEmRNAs. Three lncRNAs enriched in the PI3K-AKt signaling pathway, LINC02082, LOC105369812, and LOC105375170, were used as candidate lncRNAs. After correlation analysis with known biomarkers, LINC02082 and LOC105369812 were selected as the target lncRNAs. The qRT-PCR results showed that the target lncRNAs were significantly different among the 3 tissues. The ROC curve showed that LOC105369812 could be used to differentiate PTC from benign thyroid nodules, whereas LINC02082 and its combination had lower predictive value. Conclusions: LOC105369812 is valuable for differentiating benign from malignant thyroid nodules, whereas LINC02082 has lower diagnostic value.

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CircNf1-mediated CXCL12 expression in the spinal cord contributes to morphine analgesic tolerance

Cited by 15 publications

References 53 publications

Integrating genome-wide association study with regulatory SNP annotations identified novel candidate genes for osteoporosis

Integrating genome-wide association study with regulatory SNP annotations identified novel candidate genes for osteoporosis

Molecular and Epigenetic Aspects of Opioid Receptors in Drug Addiction and Pain Management in Sport

Significance of LINC02082 and LOC105369812 in differentiating papillary thyroid cancer from benign nodules

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