circNUDT21 promotes bladder cancer progression by modulating the miR-16-1-3p/MDM2/p53 axis

Chen, Liang; Li, Wencheng; Li, Zhiqin; Song, Yarong; Zhao, Jun; Chen, Zhaohui; Kazobinka, Gallina; Li, Lulu; Xing, Yifei; Hou, Teng

doi:10.1016/j.omtn.2021.08.032

Cited by 12 publications

(9 citation statements)

References 31 publications

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“…For instance, hsa_circ_0014130 promotes BCa cell proliferation, migration and invasion by serving as a ceRNA for miR-132-3p [33]. CircNUDT21 upregulates MDM2 expression by acting as a miR-16-1-3p sponge to promote BCa proliferation and invasion [17].…”

Section: Discussionmentioning

confidence: 99%

“…For example, circKDM4C is upregulated in BCa and mainly acts as a sponge of miR-200b-3p and miR-200c-3p to promote BCa invasion and metastasis [15]. Moreover, more circRNAs, such as circEHBP1 [16], circNUDT21 [17], and circ_0000658 [18], have been identi ed to be dysregulated in BCa. Therefore, circRNAs are promising biomarkers and valuable therapeutic targets for BCa.…”

Section: Introductionmentioning

confidence: 99%

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CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

et al. 2023

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BackgroundBladder cancer (BCa) is a malignant tumor that occurs in the bladder mucosa with high mortality. Circular RNAs (circRNAs), newly discovered noncoding RNAs, are associated with the occurrence and development of BCa. However, the effects of circRNAs in BCa have not been fully elucidated. Through the GEO (Gene Expression Omnibus) database, an abnormally expressed circular RNA, circHGS (hsa_circ_0004721), was rst identi ed in BCa. In this study, we clari ed the roles and potential mechanism of circHGS in BCa progression. MethodsThe level of circHGS in tissue and cell was assessed by qRT-PCR. The proliferation, migration and invasion of BCa cells were detected by CCK8, colony formation assay, wound-healing assay, and Transwell assays, respectively. The interaction between circHGS/VEGFC and miR-513a-5p was detected by dual luciferase and qRT-PCR. VEGFC protein level was detected by western blotting. Nude mouse xenograft model was used to clarify the role of circHGS in tumor growth in vivo. ResultsCircHGS, originating from the HGS gene, is upregulated in BCa tissues compared to normal tissues.Functionally, silencing of circHGS apparently suppressed BCa cell proliferation, migration and invasion, but circHGS overexpression showed the opposite result. In vivo experiments also suggested that knockdown of circHGS suppressed tumor growth. Mechanistically, circHGS functions as a sponge of miR-513a-5p to elevate VEGFC expression and activate the AKT/mTOR signaling pathway, ultimately promoting BCa progression. ConclusionOur ndings indicated that circHGS promotes BCa progression via the miR-513a-5p/VEGFC/AKT/mTOR pathway and can be a promising therapeutic target of BCa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

et al. 2023

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“…Additionally, MDM2 is a potential downstream target of miR-16-1-3p, and circNUDT21 promotes BCa progression by sponging miR-16-1-3p and activating the miR-16-1-3p/MDM2/p53 axis. 104 Similarly, Zhou et al found that circ_0080229 expression was upregulated in both glioma tissues and cell lines. Circ_0080229 relieved the inhibitory effect of miR-1827 on MDM2 via sponging and promoted tumorigenesis and metastasis.…”

Section: Circrna-mediated Regulation Of Cell Cycle Via P53mentioning

confidence: 94%

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

Liu¹,

Qu²,

Yang³

et al. 2022

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Circular RNAs (circRNAs) participate in the occurrence and development of various diseases through different mechanisms, such as by acting as a microRNA (miRNA) sponge, interacting with RNA-binding proteins, and regulating gene transcription and protein translation. For example, the abnormal expression of specific circRNAs in tumor cells can alter key regulatory factors and the cell cycle network, resulting in cell cycle disorders and the development and metastasis of tumors. Here, we summarize the mechanisms involved in the circRNA-mediated processes that lead to uncontrolled cell cycle and tumor cell proliferation. Extensive studies investigating the abnormal expression of circRNAs in different cancer types have been conducted. The unique characteristics of circRNAs and their ability to regulate the cell cycle through diverse mechanisms is extremely valuable in tumor diagnosis, treatment, and prognosis. Our review may assist in further understanding the circRNA-mediated regulation of the cell cycle in tumors and provide insights for research on circRNA-based therapeutic strategies and biological diagnosis for cancer.

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“…Cellular or tissue protein was extracted with RIPA lysis buffer (Servicebio, Wuhan, China), and western blot was performed as previously described [ 27 ]. Antibodies use for western blot: anti-PABPN1 (66807-1-Ig, Proteintech, USA), anti-GAPDH (60004-1-Ig, Proteintech, USA), anti-ATP6AP2 (10926-1-AP, Proteintech, USA), anti-TMEM97 (26444-1-AP, Proteintech, USA), anti-ZNF777 (NBP1-03344, Novus Biologicals, USA), anti-CDCA2 (17701-1-AP, Proteintech, USA), anti-CCND3 (26755-1-AP, Proteintech, USA), anti-AACS (ab237802, Abcam, UK), anti-β-catenin (51067-2-AP, Proteintech, USA), anti-Histone H3 (17168-1-AP, Proteintech, USA), and anti-α-Tubulin (66031-1-Ig, Proteintech, USA).…”

Section: Methodsmentioning

confidence: 99%

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

et al. 2023

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Background About 10–20% of patients with bladder cancer (BC) progress to muscle-invasive diseases, of which the underlying key molecular events have yet to be addressed. Results Here, we identified poly(A) binding protein nuclear 1 (PABPN1), a general factor of alternative polyadenylation (APA), was downregulated in BC. Overexpression and knockdown of PABPN1 significantly decreased and increased BC aggressiveness, respectively. Mechanistically, we provide evidence that the preference of PABPN1-bound polyadenylation signals (PASs) depends on the relative location between canonical and non-canonical PASs. PABPN1 shapes inputs converging on Wnt signaling, cell cycle, and lipid biosynthesis. Conclusions Together, these findings provide insights into how PABPN1-mediated APA regulation contributes to BC progression, and suggest that pharmacological targeting PABPN1 might have therapeutic potential in patients with BC.

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circNUDT21 promotes bladder cancer progression by modulating the miR-16-1-3p/MDM2/p53 axis

Cited by 12 publications

References 31 publications

CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression

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