CircRNA-5692 inhibits the progression of hepatocellular carcinoma by sponging miR-328-5p to enhance DAB2IP expression

Liu, Zhenguo; Yu, Yang; Huang, Zebing; Kong, Yi; Hu, Xingwang; Xiao, Wei; Quan, Jun; Xue, Fan

doi:10.1038/s41419-019-2089-9

Cited by 182 publications

(141 citation statements)

References 42 publications

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“…The dual-luciferase assay confirmed the direct binding of TPTEP1 and miR-328-5p in NSCLC cells. Aberrant expression of miR-328-5p has been reported in several cancer types, and was negatively regulated by circRNA (circRNA-5692) and lncRNA (LINC00210) (47,49,50). Our data implied that TPTEP1 downregulation may contribute to the increase of miR-328-5p in NSCLC, indicating TPTEP1 as a novel regulator of miR-328-5p.…”

Section: Discussionsupporting

confidence: 51%

lncRNA TPTEP1 competitively sponges miR‑328‑5p to inhibit the proliferation of non‑small cell lung cancer cells

Cao

Wang²,

Feng

et al. 2020

Oncol Rep

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Accumulating evidence suggests that lncRNAs are involved in almost all normal physiological processes and that aberrant expression of lncRNAs may be involved in the development of diseases, including non-small cell lung cancer (NSCLC). However, the roles of lncRNA-TPTE pseudogene 1 (TPTEP1) in lung cancer and the underlying molecular mechanisms have remained elusive. In the present study, significant downregulation of TPTEP1 in tumors compared with normal tissues from patients with NSCLC was observed. Overexpression of TPTEP1 inhibited cell proliferation and induced apoptosis in NSCLC cells. A bioinformatics analysis based on miRDB predicted microRNA (miR)-328-5p as a potential binding miRNA for TPTEP1. Using a dual-luciferase reporter assay and western blot analysis, it was further validated that TPTEP1 sponged miR-328-5p to upregulate Src kinase signaling inhibitor 1 (SRCIN1) in NSCLC cells. Through regulation of SRCIN1, TPTEP1 was indicated to inactivate the Src and STAT3 pathways in NSCLC cells. Notably, silencing of SRCIN1 reversed the TPTEP1 overexpression-induced inhibition of cell proliferation and increase of the apoptotic rate in NSCLC cells. Pearson correlation analysis revealed a significant positive correlation between TPTEP1 and SRCIN1 mRNA levels in NSCLC tumors. The present results provided insight into the roles of TPTEP1 in NSCLC and the underlying mechanisms.

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Section: Discussionsupporting

confidence: 51%

lncRNA TPTEP1 competitively sponges miR‑328‑5p to inhibit the proliferation of non‑small cell lung cancer cells

Cao

Wang²,

Feng

et al. 2020

Oncol Rep

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“…Moreover, a regulatory mechanism was recognized that circRNA may modulate the promoter methylation degree of its regulatory gene, thereby further to regulate its transcriptional expression [48]. For example, it was reported that circRNA-5692 decreased the methylation levels of the promoter region of the DAB2IP gene, thereby to further upregulate the expression level of the DAB2IP gene [49]. Thus, in this study, we also investigated the methylation status of CDK19 gene promoter region in SHF stem cells with overexpression or knockdown of circRNA-1926 for revealing the possible methylation events.…”

Section: Circrna-1926 Promotes Cdk19 Expression Without Modulating Thmentioning

confidence: 99%

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

Yin

Zhao

Jiang

et al. 2020

Animals

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Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5′ to 3′ free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.

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“…It is becoming increasingly evident that dysregulated circRNAs are involved in almost all types of cancers, such as hepatocellular carcinoma, bladder cancer, breast cancer, and lung cancer, the latter having the highest morbidity and mortality rates in the world [13][14][15][16][17][18] . As the most common pathological classification of lung cancer, lung adenocarcinoma, which is often asymptomatic in the early stages, causes~500,000 deaths each year 18 .…”

Section: Introductionmentioning

confidence: 99%

HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma

Zhang³

et al. 2020

Cell Death Dis

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In this study, we identified a circular form of ASPH RNA (circASPH), expression of which was upregulated in lung adenocarcinoma and the human lung adenocarcinoma cell lines. We also found a positive correlation between circASPH level and the T and N stages of lung adenocarcinoma patients. Patients with higher levels of circASPH had a shorter overall survival. Moreover, we demonstrated that circASPH was directly regulated by HMGA2 and Twist1. The direct positive regulation of circASPH by Twist1 was dependent on the presence of HMGA2. Functional assays indicated that circASPH promoted the proliferation, migration, and invasion of lung adenocarcinoma cell lines in vitro. The promoting effect of tumor growth by circASPH was also observed in vivo. Mechanistically, circASPH was identified to act as a molecular sponge for miR-370 and abrogate miR-370-mediated inhibition of HMGA2. Finally, we demonstrated that the oncogenic function of circASPH was HMGA2-dependent. These findings reveal the oncogenic functions of the HMGA2-circASPH-HMGA2 axis and may be useful in developing circRNA-based therapeutic strategies for lung adenocarcinoma.

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CircRNA-5692 inhibits the progression of hepatocellular carcinoma by sponging miR-328-5p to enhance DAB2IP expression

Cited by 182 publications

References 42 publications

lncRNA TPTEP1 competitively sponges miR‑328‑5p to inhibit the proliferation of non‑small cell lung cancer cells

lncRNA TPTEP1 competitively sponges miR‑328‑5p to inhibit the proliferation of non‑small cell lung cancer cells

CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma

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