CircRNA circFADS2 is Under-expressed in Sepsis and Protects Lung Cell From LPS-induced Apoptosis by Downregulating miR-133a

Abstract

Background: It has been reported that circFADS2 and miR-133a play opposite roles in LPS-induced cell apoptosis, which contributes to the development of sepsis. This study was carried out to explore the interaction between circFADS2 and miR-133a in sepsis. Methods: Expression of circFADS2 and miR-133a in plasma from both sepsis patients (n=62) and healthy controls (n=62) was studied by RT-qPCR. The effects of LPS on the expression of circFADS2 and miR-133a were also analyzed by RT-qPCR. The crosstalk between circFADS2 and miR-133a was analyzed by overexpression, followed by RT-qPCR. The role of circFADS2 and miR-133a in regulating the apoptosis of lung cells induced by LPS was analyzed by cell apoptosis assay.Results: We found that circFADS2 was under-expressed in sepsis and miR-133a was overexpressed in sepsis. An inverse correlation between circFADS2 and miR-133a was observed across sepsis samples. In lung cells, LPS treatment decreased the expression of circFADS2 and increased the expression of miR-133a. In lung cells, circFADS2 overexpression decreased the expression of miR-133a. Moreover, circFADS2 overexpression reduced the enhancing effects of miR-133a overexpression on cell apoptosis induced by LPS. Conclusion: Therefore, circFADS2 is under-expressed in sepsis and may protect lung cell from LPS-induced apoptosis by downregulating miR-133a.