circRNA circARNT2 Suppressed the Sensitivity of Hepatocellular Carcinoma Cells to Cisplatin by Targeting the miR-155-5p/PDK1 Axis

Li, Yueyong; Zhang, Yingjun; Zhang, Shuai; Huang, Deyou; Li, Baosheng; Liang, Gencheng; Wu, Yingning; Jiang, Qiulan; Li, Longhua; Cheng, Lin; Wei, Zhonghen; Meng, Lingzhang

doi:10.1016/j.omtn.2020.08.037

Cited by 41 publications

(30 citation statements)

References 26 publications

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“…Li et al . reported that cisplatin susceptibility was restrained by circARNT2 in hepatocellular carcinoma cells via targeting the miR-155-5p/PDK1 axis [25] . These studies supported that the dysregulated circRNAs exerted the regulatory effects on drug resistance in cancers.…”

Section: Discussionmentioning

confidence: 99%

CircRERE confers the resistance of multiple myeloma to bortezomib depending on the regulation of CD47 by exerting the sponge effect on miR-152-3p

Fang

Yang

et al. 2021

Journal of Bone Oncology

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Background Inevitable resistance to chemotherapeutic drugs has become a major obstacle for the clinical treatment of multiple myeloma (MM). Circular RNAs (circRNAs) can regulate the chemoresistance in different tumors. Our study was to explore the regulation of circRNA arginine-glutamic acid dipeptide repeats (circRERE) in bortezomib (BTZ) resistance of MM. Methods CircRERE, microRNA-152-3p (miR-152-3p) and cluster of differentiation 47 (CD47) levels were assayed through the quantitative real-time polymerase chain reaction (qRT-PCR). Cell sensitivity to BTZ was analyzed using Cell Counting Kit-8 (CCK-8) assay. Cell proliferation and apoptosis were determined via colony formation assay and flow cytometry, respectively. The detection of all proteins was conducted by western blot. The target binding was analyzed via the dual-luciferase reporter assay and RIP assay. Results We found the upregulation of circRERE in BTZ-resistant MM samples and cells. BTZ resistance was inhibited after circRERE expression was downregulated in MM cells. CircRERE was identified to act as a miR-152-3p sponge. The effect of circRERE on the BTZ resistance was associated with the sponge function for miR-152-3p. CD47 was a target for miR-152-3p and circRERE could sponge miR-152-3p to generate the expression regulation of CD47. MiR-152-3p facilitated the susceptibility of MM cells to BTZ by targeting CD47. Conclusion These results suggested that circRERE could suppress the BTZ resistance in MM cells by mediating the miR-152-3p/CD47 axis.

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Section: Discussionmentioning

confidence: 99%

CircRERE confers the resistance of multiple myeloma to bortezomib depending on the regulation of CD47 by exerting the sponge effect on miR-152-3p

Fang

Yang

et al. 2021

Journal of Bone Oncology

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“…circFN1 (hsa_circ_0058124) was found to be able to promote the expression of E2F1 by acting as a miR-1205 sponge; thus, inhibition of circFN1 could enhance the sensitivity of HCC cells to sorafenib 103 . circARNT2 (hsa_circ_0104670) regulated cisplatin resistance through the miR-155-5p/PDK1 axis, while downregulation of circARNT2 inhibited cell proliferation and enhanced the sensitivity of HCC cells to cisplatin 104 . Knockdown of circ_0003998 inhibited drug-resistant cell viability, migration, invasion and EMT, and also enhanced the toxicity of doxorubicin to HCC cells.…”

Section: The Roles Of Circrna In Hccmentioning

confidence: 99%

The functional roles, cross-talk and clinical implications of m6A modification and circRNA in hepatocellular carcinoma

Qin¹,

Mao²,

Xue³

et al. 2021

Int. J. Biol. Sci.

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Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. HCC has high rates of death and recurrence, as well as very low survival rates. N6-methyladenosine (m6A) is the most abundant modification in eukaryotic RNAs, and circRNAs are a class of circular noncoding RNAs that are generated by back-splicing and they modulate multiple functions in a variety of cellular processes. Although the carcinogenesis of HCC is complex, emerging evidence has indicated that m6A modification and circRNA play vital roles in HCC development and progression. However, the underlying mechanisms governing HCC, their cross-talk, and clinical implications have not been fully elucidated. Therefore, in this paper, we elucidated the biological functions and molecular mechanisms of m6A modification in the carcinogenesis of HCC by illustrating three different regulatory factors ("writer", "eraser", and "reader") of the m6A modification process. Additionally, we dissected the functional roles of circRNAs in various malignant behaviors of HCC, thereby contributing to HCC initiation, progression and relapse. Furthermore, we demonstrated the cross-talk and interplay between m6A modification and circRNA by revealing the effects of the collaboration of circRNA and m6A modification on HCC progression. Finally, we proposed the clinical potential and implications of m6A modifiers and circRNAs as diagnostic biomarkers and therapeutic targets for HCC diagnosis, treatment and prognosis evaluation.

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“…Since then, miRNA sponge function of circRNAs has been comprehensively investigated in many biological processes(10). Li et al revealed that circARNT2 functions as an oncogene by sponging miR-155-5p, leading to PDK1 upregulation, and nally sensitizes HCC cells to cisplatin (7). We primarily found six miRNAs in common via overlapping mAPP-CLIP seq data with circRNA binding miRNAdata in StarBase database with tight screening criteria(21).…”

Section: Discussionmentioning

confidence: 92%

CircAPP Competes with APP mRNA to Promote Malignant Progression of Bladder Cancer

Qi¹,

Cao²,

Wu³

et al. 2021

Preprint

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Background: Bladder cancer (BCa) is the most common cancer in the urinary system with high recurrence rate and poor prognosis. Circular RNA (circRNA) is a novel subclass of noncoding-RNAwhich participate in progression of BCa. Here, we identified a novel circRNA—circAPP and aimed to investigate the role of circAPP in progression of BCa.Method: Public data of RNA sequencing was used to identify significant circRNA related to BCa. The role of circRNAs in progression in BCa was assessed in cytotoxicity assay, transwell assay andflow cytometry. Biotin-coupled RNA pull-down and fluorescence in situ hybridization (FISH) were performed to evaluate the interaction between circRNAs and miRNAs. Results: The expression of circAPP was higher in BCa tissues and cells than in normal samples. In vitro experiments showed that knockdown of circAPP inhibited cell proliferation and impeded the metastasis of BCa cells. Mechanistically, we demonstrated that circAPP acts as a sponge for miR-186-5p and promotes host gene APP’s expression. Clinically, circAPP predicts worse overall survival of BCa patients, indicating its prognostic value.Conclusion: Our study identified that circAPP modulates metastasis of BCa through miR-186-5p/APP aixs and may serve as a promising prognostic biomarker for BCa, which provides novel insights into treatment of BCa.

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circRNA circARNT2 Suppressed the Sensitivity of Hepatocellular Carcinoma Cells to Cisplatin by Targeting the miR-155-5p/PDK1 Axis

Cited by 41 publications

References 26 publications

CircRERE confers the resistance of multiple myeloma to bortezomib depending on the regulation of CD47 by exerting the sponge effect on miR-152-3p

CircRERE confers the resistance of multiple myeloma to bortezomib depending on the regulation of CD47 by exerting the sponge effect on miR-152-3p

The functional roles, cross-talk and clinical implications of m6A modification and circRNA in hepatocellular carcinoma

CircAPP Competes with APP mRNA to Promote Malignant Progression of Bladder Cancer

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